Abstract：
:The formation of mesoderm is an important developmental process of vertebrate embryos, which can be broken down into several steps; mesoderm induction, patterning, morphogenesis and differentiation. Although mesoderm formation in Xenopus has been intensively studied, much remains to be learned about the molecular events responsible for each of these steps. Furthermore, the interplay between mesoderm induction, patterning and morphogenesis remains obscure. Here, we describe an enhanced functional screen in Xenopus designed for large-scale identification of genes controlling mesoderm formation. In order to improve the efficiency of the screen, we used a Xenopus tropicalis unique set of cDNAs, highly enriched in full-length clones. The screening strategy incorporates two mesodermal markers, Xbra and Xmyf-5, to assay for cell fate specification and patterning, respectively. In addition we looked for phenotypes that would suggest effects in morphogenesis, such as gastrulation defects and shortened anterior-posterior axis. Out of 1728 full-length clones we isolated 82 for their ability to alter the phenotype of tadpoles and/or the expression of Xbra and Xmyf-5. Many of the clones gave rise to similar misexpression phenotypes (synphenotypes) and many of the genes within each synphenotype group appeared to be involved in similar pathways. We determined the expression pattern of the 82 genes and found that most of the genes were regionalized and expressed in mesoderm. We expect that many of the genes identified in this screen will be important in mesoderm formation.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Chen JA,Voigt J,Gilchrist M,Papalopulu N,Amaya Edoi
10.1016/j.mod.2004.11.008subject
Has Abstractpub_date
2005-03-01 00:00:00pages
307-31issue
3eissn
0925-4773issn
1872-6356pii
S0925-4773(04)00274-6journal_volume
122pub_type
杂志文章abstract：:The synchronous development of a sibling group of Rhynchosciara larvae enables us to follow the relationship between the local transcription and extrareplication of C3 and C8 DNA puffs. Although DNA amplification at these two loci takes place during the last cycle of DNA duplication in salivary gland chromosomes, a di...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(91)90072-e
更新日期：1991-12-01 00:00:00
abstract：:Early in the development of the imaginal wing disc of Drosophila, the LIM-HD gene tailup (islet), together with the HD genes of the iroquois complex, specify the notum territory of the disc. Later, tailup has been shown to act as a prepattern gene that antagonizes formation of sensory bristles on the notum of this fly...
journal_title：Mechanisms of development
pub_type： 杂志文章
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更新日期：2010-09-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
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更新日期：1998-07-01 00:00:00
abstract：:Normal sexual differentiation in the genital organs is essential for the animal species that use sexual reproduction. Although it is known that doublesex (dsx) is required for the sexual development of the genitalia in various insect species, the direct target genes responsible for the sexual differentiation of the ge...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2017.01.001
更新日期：2017-02-01 00:00:00
abstract：:Formation of tubular structures from an epithelial tissue is a process common to many morphogenetic events during organogenesis. We report here new data concerning the expression pattern of the vHNF1/HNF1beta gene during this process in the mouse. vHNF1 (variant Hepatocyte Nuclear Factor 1) is a member of the HNF1 hom...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(99)00221-x
更新日期：1999-12-01 00:00:00
abstract：:We studied the expression of MUL, a gene encoding a novel member of the RING-B-Box-Coiled Coil family of zinc finger proteins that underlies the human inherited disorder, Mulibrey nanism. In early human and mouse embryogenesis MUL is expressed in dorsal root and trigeminal ganglia, liver and in epithelia of multiple t...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/s0925-4773(01)00491-9
更新日期：2001-10-01 00:00:00
abstract：:In the Drosophila embryonic CNS several subtypes of glial cells develop, which arrange themselves at characteristic positions and presumably fulfil specific functions. The mechanisms leading to the specification and differentiation of glial subtypes are largely unknown. By DiI labelling in glia-specific Gal4 lines we ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2007.12.004
更新日期：2008-05-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2005.01.001
更新日期：2005-06-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(92)90052-l
更新日期：1992-09-01 00:00:00
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更新日期：1996-02-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2004.04.005
更新日期：2004-05-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
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更新日期：2003-07-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2010.03.003
更新日期：2010-05-01 00:00:00
abstract：:Male and female embryonic stem (ES) cell lines were derived from the area pellucidae of Stage X (EG&K) chicken embryos. These ES cell lines were grown in culture for extended periods of time and the majority of the cells retained a diploid karyotype. When reintroduced into Stage VI-X (EG&K) recipient embryos, the cES ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2005.10.002
更新日期：2006-01-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(92)90018-f
更新日期：1992-03-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/0925-4773(94)90054-x
更新日期：1994-01-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2014.01.003
更新日期：2014-05-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2012.09.003
更新日期：2013-02-01 00:00:00
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journal_title：Mechanisms of development
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journal_title：Mechanisms of development
pub_type： 杂志文章
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journal_title：Mechanisms of development
pub_type： 杂志文章
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更新日期：2001-07-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
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更新日期：2001-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2000-12-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
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更新日期：2016-05-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章,评审
doi：10.1016/j.mod.2020.103647
更新日期：2020-12-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
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更新日期：2001-02-01 00:00:00
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journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2006.10.001
更新日期：2007-02-01 00:00:00
abstract：:The normal embryogenesis of marine animals is typically confined to a species-specific range of temperatures. Within that temperature range development results in a consistent, or canalized, phenotype, whereas above and below the range abnormal phenotypes are produced. This study reveals a high temperature threshold, ...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2019.04.002
更新日期：2019-06-01 00:00:00
abstract：:This study utilizes morphological and mechanistic endpoints to characterize the onset of bilateral atresia of the vas deferens in a recently derived cystic fibrosis (CF) rat model. Embryonic reproductive structures, including Wolffian (mesonephric) duct, Mullerian (paramesonephric) duct, mesonephric tubules, and gonad...
journal_title：Mechanisms of development
pub_type： 杂志文章
doi：10.1016/j.mod.2018.10.002
更新日期：2019-02-01 00:00:00
abstract：:The Drosophila pointed gene encodes two ETS transcriptional activators, pointedP1 and pointedP2, sharing a common C-terminal ETS domain. In the embryonic central nervous system pointedP2 is required for midline glial cell differentiation, whereas, in the eye, pointedP2 is essential for photoreceptor cell differentiati...
journal_title：Mechanisms of development
pub_type： 杂志文章
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更新日期：1996-09-01 00:00:00
