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In vivo incorporation of an alkyne into proteins in Escherichia coli.

Abstract：

:Using a genetic selection we identified mutants of the M. janaschii tyrosyl-tRNA synthetase that selectively charge an amber suppressor tRNA with para-propargyloxyphenylalanine in Escherichia coli. These evolved tRNA-synthetase pairs were used to site-specifically incorporate an alkynyl group into a protein, which was subsequently conjugated with fluorescent dyes by a [3+2]-cycloaddition reaction under mild reaction conditions.

journal_name

Bioorg Med Chem Lett

journal_title

Bioorganic & medicinal chemistry letters

authors

Deiters A,Schultz PG

doi

10.1016/j.bmcl.2004.12.065

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

1521-4

issue

5

eissn

0960-894X

issn

1464-3405

pii

S0960-894X(04)01527-6

journal_volume

15

pub_type

杂志文章

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