Abstract：
:Glycoprotein VI (GPVI) is an essential platelet receptor for collagens that is exclusively expressed in the megakaryocytic lineage. Transcription of the human gene GP6 is driven largely by GATA-binding protein 1 (GATA-1), specificity protein 1 (Sp1), and Friend leukemia integration 1 (Fli-1). In this report, we show that GPVI expression during megakaryocytic differentiation is dependent on cytosine-phosphate-guanosine (CpG) demethylation that can be initiated by thrombopoietin (TPO). Sodium bisulfite genomic sequencing established that a CpG-rich island within the GP6 promoter region is fully methylated at 10 CpG sites in GPVI-nonexpressive cell lines, such as UT-7/EPO and C8161, but completely unmethylated in GPVI-expressive cell lines, including UT-7/TPO and CHRF288-11. To further confirm the relationship between CpG demethylation and expression of GPVI in primary cells, we treated human cord blood cells with TPO. The GP6 promoter is highly methylated in cord blood mononuclear cells (progenitors) but not in CD41+-enriched cells obtained after TPO differentiation. Furthermore, when UT-7/EPO-Mpl cells, which stably express human C-myeloproliferative leukemia virus ligand (c-Mpl), were treated with TPO, demethylation of the GP6 promoter was induced. In every case, demethylation of the GP6 promoter correlated with an increase in mRNA level. Thus, megakaryocyte-specific expression of the GP6 gene is regulated, in part, by CpG demethylation, which can be directly initiated by TPO.
journal_name
Bloodjournal_title
Bloodauthors
Kanaji S,Kanaji T,Jacquelin B,Chang M,Nugent DJ,Komatsu N,Moroi M,Izuhara K,Kunicki TJdoi
10.1182/blood-2004-08-3109subject
Has Abstractpub_date
2005-05-15 00:00:00pages
3888-92issue
10eissn
0006-4971issn
1528-0020pii
2004-08-3109journal_volume
105pub_type
杂志文章相关文献
BLOOD文献大全abstract：:Twenty-one human immunodeficiency virus (HIV)-positive patients, including 11 acquired immunodeficiency syndrome (AIDS)-free patients with immune thrombocytopenic purpura (ITP), were studied to determine whether the megakaryocytic/platelet lineage was infected by HIV. Because purification of platelets did not reach a ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:The biochemical signaling mechanisms involved in transducing the effects of interferon-gamma (IFN-gamma) on human leukemia-derived HL-60 cell differentiation are not completely understood. Recent studies established the existence of a sphingomyelin (SM) cycle that operates in response to the action of IFN-gamma on HL-...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Fifty-eight acute promyelocytic leukemia (APL) patients (11 newly diagnosed and 47 relapsed) were studied for arsenic trioxide (As2O3) treatment. Clinical complete remission (CR) was obtained in 8 of 11 (72.7%) newly diagnosed cases. However, As2O3 treatment resulted in hepatic toxicity in 7 cases including 2 deaths, ...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：
更新日期：1999-11-15 00:00:00
abstract：:Fifty-four patients with severe aplastic anemia were treated with horse anti-human thymocyte globulin (ATG) and androgens. Thirty of these patients also received an infusion of HLA-haploidentical marrow cells. Only those patients having evidence of hematologic recovery within 3 mo after ATG therapy were considered res...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1984-02-01 00:00:00
abstract：:The intracellular Ca(2+) concentration of many nonexcitable cells is regulated by calcium store release and store-operated calcium entry (SOCE). In platelets, STIM1 was recently identified as the main calcium sensor expressed in the endoplasmic reticulum. To evaluate the role of the SOC channel moiety, Orai1, in plate...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2008-08-174516
更新日期：2009-01-15 00:00:00
abstract：:The tendency of 5-methylcytosine (5mC) to undergo spontaneous deamination has had a major role in shaping the human genome, and this methylation damage remains the primary source of somatic mutations that accumulate with age. How 5mC deamination contributes to cancer risk in different tissues remains unclear. Genomic ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2018-05-852566
更新日期：2018-10-04 00:00:00
abstract：:Uniformly adopted response criteria are essential for assessment of therapies incorporating conventional chemotherapy and chemoimmunotherapy regimens. Recently, immunomodulatory agents, such as immune checkpoint inhibitors, have demonstrated impressive activity in a broad range of lymphoma histologies. However, these ...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2016-05-718528
更新日期：2016-11-24 00:00:00
abstract：:Activation and subsequent differentiation of naive CD8+ T cells lead to the development of memory subsets with distinct homing and effector capacities. On nonlymphoid homing subsets, expression of "inflammatory" chemokine receptors (such as CXCR3, CCR5, CX3CR1, and CXCR1) is believed to promote migration into sites of...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-04-1366
更新日期：2005-12-01 00:00:00
abstract：:Malignant transformation by oncogenes requires additional genetic/epigenetic changes to overcome enhanced susceptibility to apoptosis. In the present study, we report that Bif-1 (Sh3glb1), a gene encoding a membrane curvature–driving endophilin protein, is a haploinsufficient tumor suppressor that plays a key role in ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-10-459826
更新日期：2013-02-28 00:00:00
abstract：:The platelets of an infant with severe combined immune deficiency and adenosine deaminase deficiency showed markedly diminished responses to ADP-induced aggregation in vitro. This abnormality was corrected by the addition of purified adenosine deaminase in vitro. Exogenous adenosine added to platelet-rich plasma cause...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：:We confirmed that murine hematopoietic stem cells express the c-kit molecule but not lymphohematopoietic lineage markers. These lineage marker-negative c-kit-positive (Lin- c-kit+) cells were further divided according to the uptake of rhodamine-123 (Rh-123). Approximately 1,000 Lin- c-kit+ rhodamine-123dull cells, whi...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:Interleukin-11 (IL-11), a pleiotropic cytokine originally isolated from a primate bone marrow stromal cell line, has been shown to stimulate T-cell-dependent B-cell maturation, megakaryopoiesis, and various stages of myeloid differentiation, but to inhibit adipogenesis. Because stromal cells are essential for the main...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Human alpha2-antiplasmin (alpha2AP), also known as alpha2-plasmin inhibitor, is the major inhibitor of the proteolytic enzyme plasmin that digests fibrin. There are 2 N-terminal forms of alpha2AP that circulate in human plasma: a 464-residue protein with Met as the N-terminus, Met-alpha2AP, and a 452-residue version w...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-12-4240
更新日期：2004-05-15 00:00:00
abstract：:Endothelial cells synthesize a heterodimeric adhesion molecule, the vitronectin receptor (VnR), which is similar to the platelet glycoprotein (GP)IIb/IIIa complex. The subunits of the endothelial VnR (VnR alpha and GPIIIa) have been studied for their ability to express alloantigens associated with platelet GPIIb and I...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:Anaplastic large-cell lymphomas (ALCLs) encompass at least 2 systemic diseases distinguished by the presence or absence of anaplastic lymphoma kinase (ALK) expression. We performed genome-wide microRNA (miRNA) profiling on 33 ALK-positive (ALK[+]) ALCLs, 25 ALK-negative (ALK[-]) ALCLs, 9 angioimmunoblastic T-cell lymp...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-08-447375
更新日期：2013-09-19 00:00:00
abstract：:We examined whether activated protein C (APC) reduces ischemia/reperfusion (I/R)-induced renal injury by inhibiting leukocyte activation. In a rat model, intravenous administration of APC markedly reduced I/R-induced renal dysfunction and histological changes, whereas intravenous administration of dansyl glutamylglycy...
journal_title：Blood
pub_type： 杂志文章,收录出版
doi：
更新日期：2000-06-15 00:00:00
abstract：:Follicular lymphoma (FL) is an indolent malignancy of germinal center B cells. Although the overall survival of FL patients has recently improved with the introduction of novel therapies, there is significant heterogeneity in patient outcome and a need for rationally designed therapeutic strategies that target disease...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2017-08-737361
更新日期：2018-02-08 00:00:00
abstract：:A role for microRNA (miRNA) has been recognized in nearly every biologic system examined thus far. A complete delineation of their role must be preceded by the identification of all miRNAs present in any system. We elucidated the complete small RNA transcriptome of normal and malignant B cells through deep sequencing ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-05-285403
更新日期：2010-12-02 00:00:00
abstract：:Cultured endothelial cells produce an extracellular matrix (ECM) to which platelets adhere and spread, ultimately resulting in platelet aggregation, thromboxane B2 production, and serotonin release. We have investigated the role of fibrinogen binding to the platelet GPIIb/IIIa complex in these reactions by comparing n...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:E-cadherin gene is often termed a "metastasis suppressor" gene because the E-cadherin protein can suppress tumor cell invasion and metastasis. Inactivation of the E-cadherin gene occurs in undifferentiated solid tumors by both genetic and epigenetic mechanisms; however, the role of E-cadherin in hematologic malignanci...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-05-15 00:00:00
abstract：:Glanzmann's thrombasthenia is an inherited bleeding disorder characterized by abnormalities of platelet membrane glycoproteins (GP) IIb and IIIa. Most patients, usually designated as type I, have been reported to have undetectable levels of GP IIb and GP IIIa with the assay used. We have used polyclonal rabbit antibod...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Dendritic cells (DCs) are antigen-presenting cells with the capacity to prime naive T cells for efficient cellular responses against pathogens such as HIV-1. DCs are also susceptible to HIV-1 infection, which may impair their ability to induce immunity. Here, we examined the ability of HIV-1-infected, in vitro-derived...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-07-2314
更新日期：2004-11-01 00:00:00
abstract：:Three Rh-related cDNAs have been isolated from a human bone marrow cDNA library and by polymerase chain reaction (PCR) amplification of human bone marrow and erythroblast mRNAs. They potentially encode a family of Rh protein isoforms that exhibit several unexpected structural properties as compared with the Rh polypep...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-08-15 00:00:00
abstract：:Survivin has been described as an important regulator of late mitosis and as antiapoptotic in various cells. In this issue of Blood, Wen and colleagues describe a megakaryocyte-specific knockout of survivin, with no influence on megakaryocyte survival, and a stimulating effect on polyploidy. ...
journal_title：Blood
pub_type： 评论,杂志文章
doi：10.1182/blood-2009-04-216713
更新日期：2009-07-02 00:00:00
abstract：:Bleeding problems are associated with defects in platelet alpha-granules, yet little is known about how these granules are formed and released. Mutations affecting VPS33B, a novel Sec1/Munc18 protein, have recently been linked to arthrogryposis, renal dysfunction, and cholestasis (ARC) syndrome. We have characterized ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-04-1356
更新日期：2005-12-15 00:00:00
abstract：:The mechanisms by which transforming growth factor beta (TGF-beta) exerts a negative effect on cell-cycle entry in primary human hematopoietic stem/progenitor cells were examined at the molecular and cellular levels. After treatment of primary human CD34+ progenitors with TGF-beta there was a decrease in the levels of...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood.v99.2.499
更新日期：2002-01-15 00:00:00
abstract：:Based on our previous demonstration that quinine could be used clinically to reverse P-glycoprotein-mediated resistance, we designed a multicenter, randomized trial aiming to determine whether quinine would improve the survival of adult patients (15-60 years old) with de novo acute myelogenous leukemia (AML). These pa...
journal_title：Blood
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1182/blood-2002-11-3419
更新日期：2003-08-15 00:00:00
abstract：:Endotoxin is a component of gram-negative bacteria that causes hematologic and immunologic changes through its induction of cytokines. Interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring inhibitor of IL-1 that competes with IL-1 for occupancy of cell-surface receptors but possesses no agonist activity....
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-11-15 00:00:00
abstract：:Small nuclear U1-RNAs (snRNAs), the spliceosome components selectively recognizing donor splice sites (5'ss), were engineered to restore correct mRNA processing in a cellular model of severe coagulation factor VII (FVII) deficiency, caused by the IVS7 9726 + 5g/a change. Three U1-snRNAs, complementary to the mutated 5...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2007-10-117440
更新日期：2008-03-01 00:00:00
abstract：:Strategies that augment a GVL effect without increasing the risk of GVHD are required to improve the outcome after allogeneic stem cell transplantation (SCT). Azacitidine (AZA) up-regulates the expression of tumor Ags on leukemic blasts in vitro and expands the numbers of immunomodulatory T regulatory cells (Tregs) in...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-09-377044
更新日期：2012-04-05 00:00:00
