Conformation-activity relationship of a novel peptide antibiotic: structural characterization of dermaseptin DS 01 in media that mimic the membrane environment.

abstract：:Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, ...

journal_title：Biopolymers

authors： Raman MC,Johnson KA,Clarke DJ,Naismith JH,Campopiano DJ

更新日期：2010-09-01 00:00:00

abstract：:The open reading frame 4 (ORF 4) gene product of barley yellow dwarf virus (BYDV) may act as a movement protein (MP) by assisting the transport of viral genomic RNA across the nuclear envelope (NE) of host plant cells. To investigate interactions between BYDV MP and the NE, wild-type and mutant open reading frame (ORF...

journal_title：Biopolymers

authors： Liu K,Xia Z,Zhang Y,Wen Y,Wang D,Brandenburg K,Harris F,Phoenix DA

更新日期：2005-10-05 00:00:00

abstract：:Hyaluronic acid (HA) is an important biomacromolecule, which fulfils a number of vital physiological functions (especially in the joint synovial fluid) and also has consumer and pharmaceutical applications. HA solution properties have already been quite thoroughly characterized in response to steady shear flows but ar...

journal_title：Biopolymers

authors： Haward SJ

更新日期：2014-03-01 00:00:00

abstract：:The mode coupling diffusion theory is applied to the derivation of local dynamics in proteins in solution. The rotational dynamics of the bonds along the protein sequence are calculated and compared to the experimentally measured nmr (15)N spin-lattice relaxation time T(1), at 36.5, 60.8, and 81.1 MHz of the vnd/NK-2 ...

journal_title：Biopolymers

authors： La Penna G,Fausti S,Perico A,Ferretti JA

更新日期：2000-08-01 00:00:00

abstract：:The methods used to study the relative reaction rates of 45 different aliphatic and aromatic carboxylic acids when coupled to resin-bound amino acid amides is described. Competition experiments involving the coupling of incoming carboxylic acids to resin-bound amino acid amides were performed. The relative composition...

journal_title：Biopolymers

authors： Acharya AN,Ostresh JM,Houghten RA

更新日期：2002-10-05 00:00:00

abstract：:The formation of the KRas4B-PDEδ complex activates different signaling pathways required for the development and maintenance of cancer. Previous experimental and theoretical studies have allowed researchers to design an inhibitor of the KRas4B-PDEδ complex, "Deltarasin." This inhibitor binds to the prenyl-binding pock...

journal_title：Biopolymers

authors： Bello M,Correa-Basurto J,Vargas-Mejía MÁ

更新日期：2019-11-01 00:00:00

abstract：:This work advances bottom-up design of bioinspired materials built from peptide-amphiphiles, which are a class of bioconjugates in which a biofunctional peptide is covalently attached to a hydrophobic moiety that drives self-assembly in aqueous solution. Specifically, this work highlights the importance of peptide con...

journal_title：Biopolymers

authors： Marullo R,Kastantin M,Drews LB,Tirrell M

更新日期：2013-09-01 00:00:00

abstract：:In this study, we identified and chemically synthesized three cationic and amphipathic peptides (Glycinin-17, BCAS-16, and BCBS-11) from soybean proteins. These peptides had high isoelectric points, high positive net charges, and included multiple hydrophobic amino acids. Subsequently, we identified multiple functions...

journal_title：Biopolymers

authors： Taniguchi M,Saito K,Nomoto T,Namae T,Ochiai A,Saitoh E,Tanaka T

更新日期：2017-07-01 00:00:00

abstract：:Nucleolar targeting peptides (NrTPs) are a family of cell penetrating peptides (CPPs) derived from crotamine, a rattlesnake venom toxin. They were named NrTPs for their remarkable nucleolus-homing properties and have been studied for their potential as drug delivery vehicles. Live cell microscopy experiments were cond...

journal_title：Biopolymers

authors： Rodrigues M,Andreu D,Santos NC

更新日期：2015-03-01 00:00:00

abstract：:Peptides, peptidomimetics, and peptide derivatives that self-assemble into fibrillar gels have received increasing interest as synthetic extracellular matrices for applications in 3D cell culture and regenerative medicine. Recently, several of these fibrillizing molecules have been functionalized with bioactive compon...

journal_title：Biopolymers

authors： Jung JP,Gasiorowski JZ,Collier JH

更新日期：2010-01-01 00:00:00

abstract：:In this article, the pulsed double electron-electron resonance in electron spin-echo (PELDOR) technique is applied to study the self-aggregation of spin-labeled zervamicin IIA, a hexadecapeptide antibiotic of fungal origin, which is known to form ion channels in a phospholipid double layer. Measurements of the ion cha...

journal_title：Biopolymers

authors： Milov AD,Tsvetkov YD,Gorbunova EY,Mustaeva LG,Ovchinnikova TV,Raap J

更新日期：2002-09-01 00:00:00

abstract：:The solution conformations of a hybrid sequence peptide related to the bee venom peptide apamin have been determined using two-dimensional 1H-nmr. Apamin is an 18 amino acid peptide containing a C-terminal helix that is stabilized by two disulfide bonds. The deletion of one residue (K4) of the N-terminal "scaffold" re...

journal_title：Biopolymers

authors： Volkman BF,Wemmer DE

更新日期：1997-04-05 00:00:00

abstract：:Glycopeptide analogues of CSF114(Glc), modified at N-terminus with new ferrocenyl carboxylic acid and a new ferrocenyl-thiphosphino amino acid, were used to implement a new electrochemical biosensor for autoantibody detection in multiple sclerosis. The ferrocenyl moiety of these "electrochemical probes" did not affect...

journal_title：Biopolymers

authors： Real-Fernández F,Colson A,Bayardon J,Nuti F,Peroni E,Meunier-Prest R,Lolli F,Chelli M,Darcel C,Jugé S,Papini AM

更新日期：2008-01-01 00:00:00

abstract：:Calcium ions (Ca(2+) ) play key roles in various fundamental biological processes such as cell signaling and brain function. Molecular dynamics (MD) simulations have been used to study such interactions, however, the accuracy of the Ca(2+) models provided by the standard MD force fields has not been rigorously tested....

journal_title：Biopolymers

authors： Yoo J,Wilson J,Aksimentiev A

更新日期：2016-10-01 00:00:00

abstract：:13C and (31)P NMR spectra of a transmembrane peptide, [1-(13)C]Ala(14)-labeled A(6-34), of bacteriorhodopsin incorporated into dimyristoylphosphatidylcholine (DMPC) bilayer were recorded to clarify its dynamics and orientation in the lipid bilayer. This peptide is shown to take an alpha-helical form both in liquid cry...

journal_title：Biopolymers

authors： Kimura S,Naito A,Tuzi S,Saitô H

更新日期：2002-02-01 00:00:00

abstract：:The study of biologically active peptides is critical to the understanding of physiological pathways, especially those involved in the development of disease. Historically, the measurement of biologically active endogenous peptides has been undertaken by radioimmunoassay, a highly sensitive and robust technique that p...

journal_title：Biopolymers

authors： Xi L,Jin Y,Parker EA,Josh P,Jones A,Wijffels G,Colgrave ML

更新日期：2012-01-01 00:00:00

abstract：:DNA sequences d-TGAGGAAAGAAGGT (a 14-mer) and d-CTCCTTTCTTCC (a 12-mer) are complementary in parallel orientation forming either Donohue (reverse Watson-Crick) base pairing at neutral pH or Hoogsteen base pairing at slightly acidic pH. The structure of the complex formed by dissolving the two strands in equimolar rati...

journal_title：Biopolymers

authors： Bhaumik SR,Chary KV,Govil G,Liu K,Miles HT

更新日期：1997-06-01 00:00:00

abstract：:The existence of an "RNA world" as an early step in the history of life increases the interest for the characterization of these biomolecules. The hairpin ribozyme studied here is a self-cleaving/ligating motif found in the minus strand of the satellite RNA associated with Tobacco ringspot virus. Surface-enhanced Rama...

journal_title：Biopolymers

authors： Percot A,Lecomte S,Vergne J,Maurel MC

更新日期：2009-05-01 00:00:00

abstract：:The conformation and intermolecular association of [D-Hyi2, L-Hyi4] meso-valinomycin [cyclo[-D-Val-D-Hyi-L-Val-L-Hyi-(D-Val-L-Hyi-L-Val-D-+ ++Hyi)2-], C60H102N6O18] in a crystal form obtained from ethanol solution has been determined by x-ray crystallography. Two depsipeptides and one ethanol molecule per asymmetric u...

journal_title：Biopolymers

authors： Pletnev VZ,Ivanov VT,Langs DA,Burkhart BM,Duax WL

更新日期：1997-11-01 00:00:00

abstract：:Fourier transform IR (FTIR) spectroscopy has been successfully applied in recent years to examine the functional and structural properties of the membrane protein rhodopsin, a prototype G protein coupled receptor. Unlike UV-visible spectroscopy, FTIR spectroscopy is structurally sensitive. It may give us both global i...

journal_title：Biopolymers

authors： Vogel R,Siebert F

更新日期：2003-01-01 00:00:00

abstract：:Recently, we isolated and purified a neutral polysaccharide (PGN) from edible fungus Pleurotus geestanus. Its structure was characterized by a range of physical-chemical methods, including high performance anion exchange chromatography, uronic acid, and protein analyses, size exclusion chromatography with ultraviolet,...

journal_title：Biopolymers

authors： Zhang M

更新日期：2010-02-01 00:00:00

abstract：:Nuclear magnetic resonance and optical experiments are combined to determine the rms amplitude of local angular motion of purines in DNA in solution. A 12 base-pair duplex DNA with the sequence d(CGCGAATTCGCG)2 is deuterated at the H8 positions of adenine and guanine by exchange with solvent at 55 degrees C. The deute...

journal_title：Biopolymers

authors： Nuutero S,Fujimoto BS,Flynn PF,Reid BR,Ribeiro NS,Schurr JM

更新日期：1994-04-01 00:00:00

abstract：:Previous studies have demonstrated that His 12 plays a major role in the pH-dependent stability of the helix formed by the isolated C-peptide (residues 1-13 of ribonuclease A). Here, amino acid replacement experiments show that His 12+ stabilizes the C-peptide helix chiefly by interacting with Phe 8. The Phe 8 ... His...

journal_title：Biopolymers

authors： Shoemaker KR,Fairman R,Schultz DA,Robertson AD,York EJ,Stewart JM,Baldwin RL

更新日期：1990-01-01 00:00:00

abstract：:In this paper, we studied the quenching mechanism of intrinsic fluorescence of type I collagen by a new type photosensitizer and fluorescence quencher, hypocrellin B (HB). It was indicated that type I collagen can emit Tyr-intrinsic fluorescence with the excitation wavelength of Tyr (lambda(ex) = 269 nm). Its fluoresc...

journal_title：Biopolymers

authors： Li YH,Yue JC,Cai GP

更新日期：1997-08-01 00:00:00

abstract：:We prepared the water soluble model peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG, to throw light on the local structure of spidroin 1 (MaSpl) protein in spider dragline silk of Nephila clavipes before and after spinning. Solution (13) C NMR showed that the conformation of the peptide in aqueous solution was essentially ...

journal_title：Biopolymers

authors： Yazawa K,Yamaguchi E,Knight D,Asakura T

更新日期：2012-06-01 00:00:00

abstract：:The magnitude and nature of lesion-induced energetic perturbations empirically correlate with mutagenicity/cytotoxicity profiles and can be predictive of lesion outcomes during polymerase-mediated replication in vitro. In this study, we assess the sequence and counterbase-dependent energetic impact of the Thymine glyc...

journal_title：Biopolymers

authors： Minetti CA,Remeta DP,Iden CR,Johnson F,Grollman AP,Breslauer KJ

更新日期：2015-09-01 00:00:00

abstract：:The elucidation of structure-function relationships in insoluble elastin is often approached using elastin-like polypeptides. In this manner, the characterization of the different regions in this extensive biopolymer may be facilitated in a "piece-wise" manner. Our solid-state NMR experiments indicate that (LGGVG)n ha...

journal_title：Biopolymers

authors： Kumashiro KK,Kurano TL,Niemczura WP,Martino M,Tamburro AM

更新日期：2003-10-01 00:00:00

abstract：:A winter flounder antifreeze polypeptide (HPLC-6) has been studied in vacuo and in aqueous solution using molecular dynamics computer simulation techniques. The helical conformation of this polypeptide was found to be stable both in vacuum and in solution. The major stabilizing interactions were found to be the main-c...

journal_title：Biopolymers

authors： McDonald SM,Brady JW,Clancy P

更新日期：1993-10-01 00:00:00

abstract：:The various motifs of RNA molecules are closely related to their structural and functional properties. To better understand the nature and distributions of such structural motifs (i.e., paired and unpaired bases in stems, junctions, hairpin loops, bulges, and internal loops) and uncover characteristic features, we ana...

journal_title：Biopolymers

authors： Zorn J,Gan HH,Shiffeldrim N,Schlick T

更新日期：2004-02-15 00:00:00

abstract：:The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. The isolation of immunoglobulin Y from the egg yolks is efficient and economical, causing minimal suffering to animals. Here we present the methodology for the production of IgY antibodies specific to Staphylococc...

journal_title：Biopolymers

authors： Walczak M,Grzywa R,Łupicka-Słowik A,Skoreński M,Bobrek K,Nowak D,Boivin S,Brown EL,Oleksyszyn J,Sieńczyk M

更新日期：2015-09-01 00:00:00