Antigen-presenting cells are targets of regulatory T cells similar to those that mediate anterior chamber-associated immune deviation.

Abstract:

PURPOSE:To identify the means by which in-vitro-generated regulatory T cells, similar to those in anterior chamber-associated immune deviation (ACAID), suppress antigen-specific T-cell responses. METHODS:T regulators (T regs), generated by stimulating ovalbumin (OVA)-specific Tcr transgenic DO11.10 T cells with OVA-pulsed, TGF-beta2-treated peritoneal exudates cells (PEC), or their supernatants were added to OVA-pulsed PEC that were used to activate DO11.10 T cells in vitro or to suppress OVA-specific delayed hypersensitivity (DH) induction in vivo. RESULTS:OVA-pulsed PECs exposed in vitro to TGF-beta-producing T regs or their supernatants failed to activate DO11.10 T cells in vitro, and suppressed DH in mice immunized with OVA plus adjuvant. CONCLUSION:T cells exposed to TGF-beta2-pretreated, antigen-pulsed PECs secrete soluble factors, including active TGF-beta that regulate OVA-specific responses by forcing antigen-presenting cells to promote deviant T-cell activation in vitro and in vivo.

journal_name

Ocul Immunol Inflamm

authors

Yokoi H,Streilein JW

doi

10.1080/09273940490895317

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

101-14

issue

2

eissn

0927-3948

issn

1744-5078

pii

T7WU5825UF96NRBF

journal_volume

12

pub_type

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