Abstract:
BACKGROUND:Apoptosis in human atherosclerotic coronary plaques possibly causes plaque destabilization by contributing to the weakening and breaking down of the fibrous cap. We tested the hypothesis that apoptosis is quantitatively increased in unstable atherosclerotic plaques. METHODS AND RESULTS:We analyzed the expression of apoptotic genes such as BAX, CASP1, FAS, FAS L, FOS, MDM2, NFkB2, P53, PCNA, TERT, and XRCC1 in coronary plaques collected with directional coronary atherectomy from 15 patients with stable angina and 15 with acute coronary syndromes without ST elevation (ACS). Total RNA was extracted and cDNA was amplified with a specific set of primers and TaqMan probes. Apoptosis was also revealed by DNA laddering. To clarify the source of mRNAs, we performed in situ reverse transcriptase-polymerase chain reaction coupled with immunocytochemistry and found a substantial overlap between the mRNAs of the above genes and vascular smooth muscle cells. Gene expression analysis showed that the proapoptotic genes (ie, BAX, CASP1, FAS, FAS L, FOS, NFkB2, P53, PCNA) were significantly more expressed (P<0.001) in ACS plaques, whereas the antiapoptotic genes (ie, MDM2, TERT, XRCC1) were more transcribed (P<0.001) in stable angina plaques. Total gDNA gel electrophoresis identified a laddering pattern in the ACS plaques as evidence of end-point apoptosis. Western blotting substantially confirmed the above data. CONCLUSIONS:Our findings support the idea that ACS plaques are committed to apoptosis through an established meshwork of gene activation and inactivation, whereas stable angina plaques retain active cell homeostasis and repair mechanisms.
journal_name
Circulationjournal_title
Circulationauthors
Rossi ML,Marziliano N,Merlini PA,Bramucci E,Canosi U,Belli G,Parenti DZ,Mannucci PM,Ardissino Ddoi
10.1161/01.CIR.0000142865.04816.89subject
Has Abstractpub_date
2004-09-28 00:00:00pages
1767-73issue
13eissn
0009-7322issn
1524-4539pii
01.CIR.0000142865.04816.89journal_volume
110pub_type
杂志文章相关文献
CIRCULATION文献大全abstract:BACKGROUND:The extent and mechanism of the antithrombotic properties of fibrin-selective and non-fibrin-selective thrombolytic agents have not yet been established. METHODS AND RESULTS:The antithrombotic, thrombolytic, fibrinogenolytic, and pharmacokinetic properties of the following substances were determined in hams...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.91.5.1330
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND:Previous experimental studies have shown that the effect of serotonin on a coronary stenosis depends on whether that stenosis is compliant or fixed. However, the relation between coronary stenosis morphology and the response to serotonin in patients with angina is not known. METHODS AND RESULTS:Using comput...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.88.4.1518
更新日期:1993-10-01 00:00:00
abstract:BACKGROUND:The Doppler wire-derived (relative) coronary flow velocity reserve (CVR) that is used to evaluate functional significance of a coronary stenosis is a method performed only by interventional cardiologists. An angiographic method would be useful in the diagnostic catheterization laboratory. For this purpose, w...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.CIR.0000074279.44131.DE
更新日期:2003-06-24 00:00:00
abstract:BACKGROUND:Despite successful repair of coarctation of the aorta in childhood, adult survivors often have hypertension at rest or on exercise, and their life expectancy is shorter than normal because of premature coronary and cerebrovascular disease. This may be related to persistent structural and functional arterial ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.89.4.1745
更新日期:1994-04-01 00:00:00
abstract:BACKGROUND:We developed a computer model to predict lysis rates of thrombi for intravenous thrombolytic regimens based on the convective/diffusive penetration of reacting and adsorbing fibrinolytic species from the circulation into the proximal face of a dissolving clot. METHODS AND RESULTS:Solution of a one-compartme...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.94.4.763
更新日期:1996-08-15 00:00:00
abstract:BACKGROUND:Postprandial hyperglycemia may be a risk factor for cardiovascular disease. We compared the effects of two insulin secretagogues, repaglinide and glyburide, known to have different efficacy on postprandial hyperglycemia, on carotid intima-media thickness (CIMT) and markers of systemic vascular inflammation i...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1161/01.CIR.0000134501.57864.66
更新日期:2004-07-13 00:00:00
abstract:BACKGROUND:Rapamycin (sirolimus)-eluting stents are associated with reduced restenosis rates in animal studies and initial human trials. The present study evaluated whether orally administered everolimus (a macrolide of the same family as sirolimus) inhibits in-stent neointimal growth in rabbit iliac arteries. METHODS...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.0000033973.06059.04
更新日期:2002-10-29 00:00:00
abstract:BACKGROUND:Cardiac parasympathetic nerves run alongside the superior vena cava (SVC) and accumulate particularly epicardially adjacent to the orifice of the coronary sinus (CS). In animals, these nerves can be electrically stimulated inside the SVC or CS, which results in negative chronotropic/dromotropic effects and n...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/hc4501.099307
更新日期:2001-11-13 00:00:00
abstract::We reviewed 35 patients undergoing resection of a chronic postinfarction left ventricular aneurysm. There was 5.7% operative mortality. Of the long-term survivors, 80% are functional class I or II. Ten patients had postoperative left ventriculograms studied by means of a computerized analysis of endocardial motion usi...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND:Erythropoietin (Epo) receptors (EpoRs) are expressed in the heart. We have recently demonstrated that the endogenous Epo-EpoR system plays an important protective role in myocardial ischemia in mice and humans. In the present study, we tested our hypothesis that the endogenous Epo-EpoR system in nonhematopoi...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.106.659037
更新日期:2007-04-17 00:00:00
abstract::The left anterior descending coronary artery of four dogs and the right common carotid artery of 15 rabbits were subjected to 40-60% reduction in transluminal diameter for 1 hour by partial ligation with suture thread. Scanning electron microscopic examination of the luminal surface of these vessels revealed endotheli...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.63.3.476
更新日期:1981-03-01 00:00:00
abstract:BACKGROUND:This study was performed to determine whether antibodies against the amino-terminus of the beta-chain of fibrin (anti-beta) could noninvasively distinguish actively enlarging thrombi from thrombi stabilized with anticoagulants. METHODS AND RESULTS:Dogs with unilateral femoral vein thrombi were allocated int...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.96.9.3173
更新日期:1997-11-04 00:00:00
abstract:BACKGROUND:The morphological hallmark of tetralogy of Fallot is controversial, with much disagreement as to whether the subpulmonary infundibulum in this lesion is hypoplastic. In addition, few quantitative data are available regarding the morphometry of the subpulmonary infundibulum, what anatomic characteristics are ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.92.4.886
更新日期:1995-08-15 00:00:00
abstract::To assess the impact of operative reduction of left ventricular outflow obstruction in hypertrophic cardiomyopathy, measurements of great cardiac vein flow, oxygen and lactate content, left ventricular pressures, and cardiac index were measured at rest and during pacing stress in 20 consecutive patients (13, myotomy-m...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.79.4.766
更新日期:1989-04-01 00:00:00
abstract:BACKGROUND:Restenosis after initial, successful percutaneous transluminal coronary angioplasty (PTCA) is due to fibrocellular proliferation. METHODS AND RESULTS:The present study focused on the nature of fibrocellular tissue in humans by use of immunocytochemical techniques. Four hearts (five coronary arteries) were i...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.83.4.1327
更新日期:1991-04-01 00:00:00
abstract:BACKGROUND:While previous clinical studies have shown a possible beneficial effect of the reperfusion performed at a relatively late phase of acute myocardial infarction ("late reperfusion") in preventing left ventricular enlargement, the mechanism has not been clarified. METHODS AND RESULTS:Of 89 patients with an ini...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.88.6.2565
更新日期:1993-12-01 00:00:00
abstract:BACKGROUND:Uncertainty exists regarding the frequency of early occlusion when the left internal mammary artery (LIMA) is anastomosed to the left anterior descending artery (LAD) through a sternotomy with conventional coronary artery bypass grafting (CABG). The issue has gained importance for comparison with less invasi...
journal_title:Circulation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1161/01.cir.100.23.2353
更新日期:1999-12-07 00:00:00
abstract::From January 1968 to June 1975 tricuspid regurgitation was encountered in 238 patients of a total of 1074 patients undergoing operations on the mitral valve. During this time tricuspid annuloplasty (TA) was performed in 137 patients and the tricuspid valve was replaced (TVR) in 101 patients. Comparison of hospital mor...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1976-12-01 00:00:00
abstract:BACKGROUND:Mildly oxidized LDL (moxLDL) is thought to play a role in atherogenesis. MoxLDL induces derivatization of cell proteins and triggers a variety of intracellular signaling. We aimed to investigate whether moxLDL-induced protein derivatization may influence the activity of platelet-derived growth factor recepto...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/hc4001.097179
更新日期:2001-10-09 00:00:00
abstract:BACKGROUND:The diagnosis of long QT syndrome (LQTS) is rather straightforward. We were surprised by realizing that, despite long-standing experience, we were making occasional diagnostic errors by considering as affected subjects who, over time, resulted as not affected. These individuals were all actively practicing s...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.120.048916
更新日期:2020-12-22 00:00:00
abstract:BACKGROUND:The strategy for treating patients with severe asymptomatic carotid artery stenosis and cardiac disease remains unresolved. Staged or combined carotid endarterectomy in these patients offers the potential benefit of decreased neurological morbidity during and after cardiac surgery; however, in high-risk pati...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.106.658625
更新日期:2007-10-30 00:00:00
abstract:BACKGROUND:Heart failure is a leading cause of death worldwide. Cyclic nucleotide phosphodiesterases (PDEs), through degradation of cyclic nucleotides, play critical roles in cardiovascular biology and disease. Our preliminary screening studies have revealed PDE10A upregulation in the diseased heart. However, the roles...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.119.042178
更新日期:2020-01-21 00:00:00
abstract::In this double-blind randomized study, 19 patients with acute transmural myocardial infarction were treated with methylprednisolone administered 4.4 +/- 0.7 hours (+/- SEM) after the onset of chest pain, and were compared with 21 patients who received placebo 4.5 +/- 0.4 hours after the start of clinical symptoms. The...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1161/01.cir.65.6.1106
更新日期:1982-06-01 00:00:00
abstract:BACKGROUND:Guidelines for the management of patients with suspected coronary artery disease (CAD) rely on the age, sex, and angina typicality-based pretest probabilities of angiographically significant CAD derived from invasive coronary angiography (guideline probabilities). Reliability of guideline probabilities has n...
journal_title:Circulation
pub_type: 杂志文章,多中心研究
doi:10.1161/CIRCULATIONAHA.111.039255
更新日期:2011-11-29 00:00:00
abstract:BACKGROUND:Up to 30% of patients with end-stage heart failure experience biventricular failure that requires biventricular mechanical support. For these patients, only bulky extracorporeal or implantable displacement pumps or the total artificial heart have been available to date, which enables only limited quality of ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.110.011502
更新日期:2011-09-13 00:00:00
abstract:BACKGROUND:Oxygen administration is currently used in clinical medicine to improve peripheral oxygen delivery to tissues threatened by ischemia. However, conflicting results have been reported on the effects of oxygen in ischemic areas. This study was aimed at investigating the effects of 40% oxygen inhalation on the s...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.86.3.878
更新日期:1992-09-01 00:00:00
abstract::Expansion (regional dilatation and thinning) of acutely infarcted myocardium in man has been shown to correlate with overall cardiac dilatation and rupture. We studied gross and histopathologic features and the time course of expansion in rats. Infarcts were produced in 84 rats by ligation of the left coronary artery ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.65.7.1446
更新日期:1982-06-01 00:00:00
abstract:BACKGROUND:Since Mexican Americans have adverse patterns of risk factors for myocardial infarction relative to non-Hispanic whites, the incidence of myocardial infarction should be greater among Mexican Americans than among non-Hispanic whites. This expectation conflicts with reports generated from death certificate re...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.95.6.1433
更新日期:1997-03-18 00:00:00
abstract:BACKGROUND:Several studies have observed an inverse association between height and risk for coronary disease, but it is unclear whether other traditional coronary disease risk factors may have confounded this association. We examined the original Framingham Heart Study cohort to determine whether short stature is assoc...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.90.5.2241
更新日期:1994-11-01 00:00:00
abstract:BACKGROUND:The clinical profiles and outcomes of patients treated surgically for acute type B aortic dissection (ABAD) are often reported for those in small series or for those cared for at a single institution over a long time period, during which a continuous evolution in techniques has occurred. Accordingly, we soug...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.105.000620
更新日期:2006-07-04 00:00:00