Abstract:
:Time-resolved fluoroimmunoassay was developed for the detection of synovial-type phospholipase A2 (s-PLA2) in human serum. This solid-phase, sandwich assay uses a polyclonal rabbit antibody raised against synovial-type group II PLA2 produced in Escherichia coli. No cross-reactions were detected between s-PLA2 and PLA2 from human or porcine pancreas, human ascitic fluid, or bee or cobra venom. In healthy individuals, the average concentration of s-PLA2 is 3.7 micrograms/L, with a 95% reference interval from 1.3 to 10.8 micrograms/L. We investigated pancreatic PLA2, which is a group I PLA2, and synovial-type group II PLA2 in sera of patients with hematological malignancies and septic fever. The concentration of s-PLA2 was increased in patient sera and correlated significantly with the catalytic activity of PLA2 and the concentration of C-reactive protein. No correlation with the concentration of pancreatic PLA2 was found. The results suggest that the increased catalytic activity of PLA2 in sera of patients with septic fever results from synovial-type group II PLA2.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Nevalainen TJ,Kortesuo PT,Rintala E,Märki Fsubject
Has Abstractpub_date
1992-09-01 00:00:00pages
1824-9issue
9eissn
0009-9147issn
1530-8561journal_volume
38pub_type
杂志文章abstract::A highly sensitive two-site enzyme immunoassay (Capcellia) was developed to determine the concentration of CD4 and CD8 molecules expressed on the surface of human T lymphocytes. This assay, performed in one step (20 min), involves the specific immunocapture of T lymphocytes and reaction of the CD4 or CD8 molecules wit...
journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1980-06-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
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更新日期:1985-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1981-07-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1991-10-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1991-04-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1976-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
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更新日期:2021-01-08 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1994-07-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
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doi:
更新日期:1998-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
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doi:10.1093/clinchem/hvaa134
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1986-07-01 00:00:00
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更新日期:1991-11-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1982-06-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1985-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1986-08-01 00:00:00
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