Abstract:
:In the present study, a real-time PCR protocol was developed for the detection of macrolide resistance determinants and was validated in a nationwide study in Germany covering a total of 236 Streptococcus pyogenes and 241 Streptococcus pneumoniae strains collected from children < or = 16 years of age with community-acquired infections. Macrolide resistance was observed in 19.9% of pneumococcal strains and 14% of S. pyogenes isolates. Of the erythromycin A-resistant S. pyogenes strains, 93.9% showed the efflux type mef(A); 62.5% of the S. pneumoniae strains were mef(A)- and 37.5% erm(B)-positive. The correlation of the results of real-time PCR assay genotyping in the present study compared with those of conventional PCR genotyping and resistance phenotyping was 100%. Macrolide resistance is of growing concern in Germany. This highly sensitive and specific PCR assay to detect macrolide resistance has the potential to provide sufficiently rapid results to improve antibiotic treatment of streptococcal infections.
journal_name
Int J Antimicrob Agentsjournal_title
International journal of antimicrobial agentsauthors
Reinert RR,Franken C,van der Linden M,Lütticken R,Cil M,Al-Lahham Adoi
10.1016/j.ijantimicag.2004.02.020subject
Has Abstractpub_date
2004-07-01 00:00:00pages
43-7issue
1eissn
0924-8579issn
1872-7913pii
S0924857904001189journal_volume
24pub_type
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journal_title:International journal of antimicrobial agents
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journal_title:International journal of antimicrobial agents
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