Abstract:
PURPOSE:To examine whether indocyanine green (ICG) dye induces apoptosis in human retinal pigment epithelial (RPE) cells. DESIGN:Laboratory investigation. METHODS:Pure cultures of human RPE cells were isolated. Retinal pigment epithelial cells were incubated with different concentrations of ICG dye (1 mg/ml, 5 mg/ml, or 20 mg/ml) for 30 minutes. The rate of RPE cell apoptosis was assessed with Annexin V-FITC staining and propidium iodide (PI) by flow cytometry. RESULTS:Retinal pigment epithelial cells maintained their monolayer morphology after incubation with ICG dye. However, ICG induced a statistically significant amount of apoptosis in RPE cells at all the concentrations (1 mg/ml, 5 mg/ml, and 20 mg/ml) after 30 minutes of incubation (P <.05). The solvent solution alone (without the ICG dye) did not induce any significant apoptosis in RPE cells, when compared with culture medium. CONCLUSIONS:The incubation of RPE cells with ICG dye increased the number of apoptotic RPE cells in vitro. Our findings indicate that the decision over the intravitreal application of ICG dye needs to be made with caution.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
Rezai KA,Farrokh-Siar L,Ernest JT,van Seventer GAdoi
10.1016/j.ajo.2003.11.016subject
Has Abstractpub_date
2004-05-01 00:00:00pages
931-3issue
5eissn
0002-9394issn
1879-1891pii
S0002939403014235journal_volume
137pub_type
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