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The effect of cigarette smoke exposure on pulmonary metastatic disease in a murine model of metastatic breast cancer.

Abstract：

INTRODUCTION:Women who smoke have a higher rate of fatal breast cancer than nonsmoking women. An association between smoking and pulmonary metastases from breast cancer has been suggested by epidemiologic studies. STUDY OBJECTIVES:To examine the relationship between exposure to cigarette smoke and pulmonary metastasis in a murine model of metastatic mammary cancer. STUDY DESIGN:Prospective, randomized study. SETTING:Animal research laboratory. EXPERIMENTAL SUBJECTS:Female sexually mature BALB/cAnN mice. INTERVENTIONS:Mice were randomly divided into experimental and control groups. Experimental animals were exposed to cigarette smoke in specialized exposure chambers, at concentrations chosen to approximate active cigarette smoking. Control animals were exposed to filtered air. One week after the initiation of exposures, mouse mammary tumor cells (tumor cell line 4526) were injected into the tail veins of experimental animals at one of three concentrations (50,000, 100,000, or 150,000 cells per 100 micro L). Three weeks later, the mice were killed, and pulmonary metastases were counted and measured. RESULTS:The mean metastatic burden in the lungs was consistently greater for smoke-exposed animals at each concentration of cells injected (at 50,000 cells per 100 micro L, 9.8 vs 4.8 micro m(3), respectively [p < 0.01]; at 100,000 cells per 100 micro L, 34.5 vs 17.4 micro m(3), respectively [p < 0.10]; and at 150,000 cells per 100 micro L, 54.0 vs 31.5 micro m(3), respectively [p < 0.05]). This was largely attributable to a significant increase in the number of metastatic nodules per animal (at 50,000 cells per 100 micro L, 8.7 vs 4.8, respectively [p < 0.001]; at 100,000 cells per 100 micro L, 24.3 vs 14.0, respectively [p > 0.10]; and at 150,000 cells per 100 micro L, 42.0 vs 20.1, respectively [p < 0.02]) rather than to a change in nodule size. CONCLUSIONS:Cigarette smoke exposure is associated with an increase in the total pulmonary metastatic burden in this murine model of metastatic mammary cell cancer. This study provides experimental support for an adverse effect of smoking on the metastatic process and suggests a possible mechanism for smokers' increased breast cancer mortality.

journal_name

Chest

journal_title

Chest

authors

Murin S,Pinkerton KE,Hubbard NE,Erickson K

doi

10.1378/chest.125.4.1467

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

1467-71

issue

eissn

0012-3692

issn

1931-3543

pii

S0012-3692(15)32111-5

journal_volume

125

pub_type

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The effect of cigarette smoke exposure on pulmonary metastatic disease in a murine model of metastatic breast cancer.