Abstract:
BACKGROUND:In acute or chronic inflammatory bowel disease (IBD) interferon gamma (IFNgamma) is still considered to be an important pro-inflammatory mediator. In the present study we investigated the impact of IFNgamma on interleukin-8 (IL-8) production as a read-out for cell activation in intestinal epithelial cell (IEC) lines and primary human colonic epithelial cells (CEC). METHODS:Primary cultures of human CEC were established from the mucosa of patients without inflammatory disease. CEC, HT-29 or Caco-2 cells were incubated with either IFNgamma, tumor necrosis factor (TNF)alpha or IL-10. IL-8 and IL-1Ra secretion was determined by ELISA. Competicon PCR was used for quantification of IL-8mRNA. Apoptosis was quantified by propidium iodine incorporation and fluorescence activated cell sorting (FACS) analysis. RESULTS:In contrast to HT-29 cells in primary human CEC 100 U/ml IFNgamma inhibited IL-8 secretion significantly to 70+/-15% of unstimulated primary CEC (p<0.005) more effectively than IL-10 (87+/-21% versus unstimulated cells, n.s.). In HT-29 cells, IL-8 secretion was induced to 405+/-101% of unstimulated cells. In Caco-2 cells, IFNgamma had no significant effect on IL-8 secretion. The effect in HT-29 and CEC was concentration dependent. In primary CEC, 200 U/ml IFNgamma further reduced IL-8 secretion to 48+/-18% of unstimulated CEC (p<0.05). Whereas IL-8 mRNA was strongly upregulated in HT-29 cells, no upregulation or even a downregulation was found in CEC. Pre-incubation with 100 U/ml IFNgamma did not increase the susceptibility to apoptosis mediated by anti-Fas antibody (CH-11) in primary CEC, whereas HT-29 cells showed increased rates of apoptosis after priming with IFNgamma. CONCLUSION:In contrast to HT-29, IFNgamma downregulated IL-8 secretion and did not induce IL-8 mRNA expression in primary human CEC. This effect was not due to induction of apoptosis.
journal_name
Int J Colorectal Disjournal_title
International journal of colorectal diseaseauthors
Schlottmann K,Wachs FP,Grossmann J,Vogl D,Maendel M,Falk W,Schölmerich J,Andus T,Rogler Gdoi
10.1007/s00384-003-0570-4subject
Has Abstractpub_date
2004-09-01 00:00:00pages
421-9issue
5eissn
0179-1958issn
1432-1262journal_volume
19pub_type
杂志文章abstract:PURPOSE:Adenoma detection in colorectal cancer survivors is poorly characterised with insufficient evidence to inform frequency of surveillance schedule. The aim of this study was to examine adenoma incidence and recurrence in patients who have undergone colorectal cancer resection with curative intent. Survival outcom...
journal_title:International journal of colorectal disease
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abstract:INTRODUCTION:When 'whole colonic imaging' is indicated, clinicians must decide between optical colonoscopy, barium enema and CT colonography (CTC). CTC is a relatively new technique which has become increasingly accessible in the UK over the past 5 years. As radiologists have gained experience and scanning parameters h...
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abstract:PURPOSE:Compared with the open approach, laparoscopic total mesorectal excision (TME) achieves faster patient recovery, reduces morbidity rates, and shortens hospital stay. However, in laparoscopic low anterior resection (L-LAR), conversion to open surgery is required in almost 20% of cases. Transanal TME (Ta-TME) comb...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,多中心研究,随机对照试验
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journal_title:International journal of colorectal disease
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pub_type: 杂志文章
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journal_title:International journal of colorectal disease
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更新日期:1991-08-01 00:00:00
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更新日期:1990-08-01 00:00:00
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更新日期:2019-03-01 00:00:00
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journal_title:International journal of colorectal disease
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更新日期:2019-09-01 00:00:00
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更新日期:2019-01-01 00:00:00
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更新日期:2012-10-01 00:00:00
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更新日期:2016-06-01 00:00:00
abstract::It has been suggested that perineal descent causes puborectalis neuropathy. To elucidate this, perineal descent was measured on standard proctograms and prolongation of mean motor unit potential duration was used as the index of denervation of the external sphincter and puborectalis in 9 male and 18 female patients wi...
journal_title:International journal of colorectal disease
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更新日期:1986-04-01 00:00:00
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更新日期:2013-01-01 00:00:00
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journal_title:International journal of colorectal disease
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journal_title:International journal of colorectal disease
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更新日期:2009-01-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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更新日期:2010-03-01 00:00:00