当前位置： SCI文献检索 > AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY期刊下所有文献 > Magnesium sulfate induces translocation of protein kinase C isoenzymes alpha and delta in myometrial cells from pregnant women.

Magnesium sulfate induces translocation of protein kinase C isoenzymes alpha and delta in myometrial cells from pregnant women.

Abstract：

OBJECTIVES:The purpose of this study was to determine the effect of magnesium sulfate on protein kinase C (PKC) translocation in myometrial cells from pregnant women. STUDY DESIGN:Myometrium was obtained at the time of cesarean delivery from women at term before labor. Cultured myometrial cells were treated with magnesium sulfate (3, 5, and 10 mmol/L), oxytocin (0.1 micromol/L), or 12-O-tetradecanoylphorbol-13-acetate (TPA, 0.1 micromol/L). The translocation of PKC isozymes alpha (calcium dependent) and delta (calcium independent) from cytosol to membrane fractions was assessed with use of Western blot analysis. RESULTS:In unexposed control cells, the majority of PKC alpha and delta was located in the cytosol fraction. Exposure to magnesium sulfate for 60 minutes induced translocation of both PKC alpha and delta at concentrations as low as 5 and 3 mmol/L, respectively. The magnitude of magnesium sulfate induced translocation for PKC delta is similar to that seen after oxytocin or TPA exposure but less for PKC alpha. Exposure to oxytocin for 30 minutes and 60 minutes induced translocation of PKC alpha and delta, respectively. Exposure to TPA for 5 and 30 minutes induced translocation of PKC alpha and PKC delta, respectively. In calcium-free media, only TPA induced translocation of these two isoenzymes. CONCLUSION:Magnesium sulfate stimulates PKC translocation in cultured myometrial cells from pregnant women. Magnesium sulfate and oxytocin require extracellular calcium to induce translocation of both PKC alpha and delta.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Bermeo ME,Fomin VP,Ventolini G,Gibbs SG,McKenna DS,Hurd WW

doi

10.1016/j.ajog.2003.09.009

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

522-7

issue

2

eissn

0002-9378

issn

1097-6868

pii

S0002937803011189

journal_volume

190

pub_type

杂志文章

相关文献

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY文献大全
  • Long-term survival after chemotherapy for advanced epithelial ovarian carcinoma.

    abstract：:Four hundred twenty-nine patients were entered into four prospective randomized clinical studies between January 1, 1973, and July 1, 1979. The records of 395 of these were analyzed to determine the proportion of patients surviving 48 months after therapy initiation. Ninety-six patients (24%) were living at 48 months,...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章

    doi：10.1016/0002-9378(84)90543-x

    authors： Wharton JT,Edwards CL,Rutledge FN

    更新日期：1984-04-01 00:00:00

  • Vasoactive intestinal polypeptide and other preprovasoactive intestinal polypeptide-derived peptides in the female and male genital tract: localization, biosynthesis, and functional and clinical significance.

    abstract：:Vasoactive intestinal polypeptide, a neuropeptide with wide distribution in the central and peripheral nervous system, has a broad spectrum of biologic actions. The demonstration of vasoactive intestinal polypeptide containing nerve fibers within the female and male genital tract 17 years ago indicated a putative role...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/0002-9378(95)90506-5

    authors： Ottesen B,Fahrenkrug J

    更新日期：1995-05-01 00:00:00

  • The predictive significance of biologic risk factors for deficits in children of a high-risk population.

    abstract：:Two hundred forty-two high-risk children and 47 low-risk children were prospectively studied to assess the relationship between biologic risk factors that occurred during the perinatal period and the motor and cognitive deficits that were identified during the first year of life. Multiple risk factors were present in ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(83)90867-0

    authors： Low JA,Galbraith RS,Muir DW,Killen HL,Pater EA,Karchmar EJ

    更新日期：1983-04-15 00:00:00

  • Risk factors for maternal and fetal outcome in pregnancy complicated by Ebstein anomaly.

    abstract：OBJECTIVE:The goal of the study was to examine risks in pregnancy in patients with Ebstein anomaly. STUDY DESIGN:Data were examined retrospectively for 13 patients (27 pregnancies, 21 live births) with Ebstein anomaly during pregnancy who were treated at our institution from 1985 to 2011. The associated anomalies in t...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2013.07.005

    authors： Katsuragi S,Kamiya C,Yamanaka K,Neki R,Miyoshi T,Iwanaga N,Horiuchi C,Tanaka H,Yoshimatsu J,Niwa K,Ikeda T

    更新日期：2013-11-01 00:00:00

  • Objective structured assessment of technical skills for repair of fourth-degree perineal lacerations.

    abstract：OBJECTIVE:The objective of this study was to assess the reliability and validity of an objective structured assessment of technical skills (OSATS) for repair of fourth-degree obstetric lacerations on a surgical model. STUDY DESIGN:Three blinded judges reviewed recordings of 20 junior and 20 senior obstetrics-gynecolog...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2008.07.054

    authors： Siddiqui NY,Stepp KJ,Lasch SJ,Mangel JM,Wu JM

    更新日期：2008-12-01 00:00:00

  • Associations of gestational cardiovascular health with pregnancy outcomes: the Hyperglycemia and Adverse Pregnancy Outcome study.

    abstract：BACKGROUND:The American Heart Association's formal characterization of cardiovascular health combines several metrics in a health-oriented, rather than disease-oriented, framework. Although cardiovascular health assessment during pregnancy has been recommended, its significance for pregnancy outcomes is unknown. OBJEC...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2020.07.053

    authors： Perak AM,Lancki N,Kuang A,Labarthe DR,Allen NB,Shah SH,Lowe LP,Grobman WA,Scholtens DM,Lloyd-Jones DM,Lowe WL Jr,Hyperglycemia and Adverse Pregnancy Outcome (HAPO) Study Cooperative Research Group.

    更新日期：2020-08-05 00:00:00

  • Lymphocyte activity in the presence of peritoneal fluid from fertile women and infertile women with and without endometriosis.

    abstract：:Peritoneal fluid from women with endometriosis, unexplained infertility, and fertile controls were compared to one another and to normal human serum for effects on lymphocyte proliferation in vitro. Peritoneal fluid samples were also assayed for both interleukin-1 and interleukin-2. All peritoneal fluid samples signif...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(89)90736-9

    authors： Hill JA,Anderson DJ

    更新日期：1989-10-01 00:00:00

  • Paraaortic lymph node sampling by means of an extraperitoneal approach with a supraumbilical transverse "sunrise" incision.

    abstract：OBJECTIVES:Extraperitoneal approaches to removal of lymph nodes for staging in cervical cancer patients are numerous, and each has disadvantages. We developed a supraumbilical transverse incision to initiate irradiation within days of cervical cancer staging. STUDY DESIGN:Twenty patients with advanced stage IIB or III...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(93)90080-3

    authors： Gallup DG,King LA,Messing MJ,Talledo OE

    更新日期：1993-08-01 00:00:00

  • Vitamin D deficiency in gestational diabetes mellitus and the role of the placenta.

    abstract：OBJECTIVE:The aim of this study was to evaluate the relationships between maternal vitamin D levels and gestational diabetes mellitus (GDM) and differences in the placental production of vitamin D receptor (VDR), CYP24A, and CYP27B1. STUDY DESIGN:Forty normal pregnant women and 20 women with GDM were included in this ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2013.08.015

    authors： Cho GJ,Hong SC,Oh MJ,Kim HJ

    更新日期：2013-12-01 00:00:00

  • Acidemia in neonates with a 5-minute Apgar score of 7 or greater - What are the outcomes?

    abstract：BACKGROUND:The Apgar score is universally used for fetal assessment at the time of birth, whereas, the collection of fetal cord blood gases is performed commonly in high-risk situations or in the setting of Apgar scores of <7, which is a less standardized approach. It has been well-established that neonatal acidemia at...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2016.05.035

    authors： Sabol BA,Caughey AB

    更新日期：2016-10-01 00:00:00

  • Evaluation of an impedance measurement technique for uterine contraction recording.

    abstract：:An electronic system for the detection of uterine contractions by impedance measurement was evaluated in 30 subjects during the first stage of labor. The advantage of the system was patient comfort and ease of application of the recording electrodes. Simultaneous recordings of uterine contraction activity with convent...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(77)90586-5

    authors： Moss PL,Dmytrus KC,Roux JF

    更新日期：1977-10-15 00:00:00

  • Occupational exposures among nurses and risk of spontaneous abortion.

    abstract：OBJECTIVE:We investigated self-reported occupational exposure to antineoplastic drugs, anesthetic gases, antiviral drugs, sterilizing agents (disinfectants), and X-rays and the risk of spontaneous abortion in US nurses. STUDY DESIGN:Pregnancy outcome and occupational exposures were collected retrospectively from 8461 ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2011.12.030

    authors： Lawson CC,Rocheleau CM,Whelan EA,Lividoti Hibert EN,Grajewski B,Spiegelman D,Rich-Edwards JW

    更新日期：2012-04-01 00:00:00

  • A survey of recommendations by gynecologists in Canada regarding oral contraceptive use in the perioperative period.

    abstract：OBJECTIVE:This survey was conducted to determine the practice patterns of gynecologists in Canada regarding the discontinuation of oral contraceptives before gynecologic surgical procedures. STUDY DESIGN:In May and June of 1997, surveys were sent to all obstetricians and gynecologists on the mailing list of the Societ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1067/mob.2002.127372

    authors： Oakes J,Hahn PM,Lillicrap D,Reid RL

    更新日期：2002-12-01 00:00:00

  • Outcomes of transvaginal uterosacral ligament suspension: systematic review and metaanalysis.

    abstract：:This systematic review of uterosacral ligament suspension provides a metaanalysis of anatomic outcomes and a summary of subjective outcomes. A successful anatomic outcome was considered present when women had "optimal" or "satisfactory" (pelvic organ prolapse quantification system stage 0 or 1) outcomes. In the anteri...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.ajog.2009.07.052

    authors： Margulies RU,Rogers MA,Morgan DM

    更新日期：2010-02-01 00:00:00

  • The acute effect of hysterectomy on ovarian function.

    abstract：:The acute effect of abdominal and vaginal hysterectomy on the ovarian production of estradiol-17-beta (E2) and progesterone (P) was studied in a group of patients undergoing hysterectomy for various gynecologic indications. Plasma levels of steroids as well as gonadotropins (FSH and LH) were measured by RIA. There was...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(75)90638-9

    authors： Stone SC,Dickey RP,Mickal A

    更新日期：1975-01-15 00:00:00

  • Factors associated with preterm birth in Cardiff, Wales. II. Indicated and spontaneous preterm birth.

    abstract：OBJECTIVE:Our purpose was to examine and contrast associations of risk factors with spontaneous preterm birth and indicated preterm birth. STUDY DESIGN:Separate multiple logistic regression analyses were performed of indicated and spontaneous preterm births in a large database of births in Cardiff, Wales. RESULTS:Spo...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(95)90288-0

    authors： Meis PJ,Michielutte R,Peters TJ,Wells HB,Sands RE,Coles EC,Johns KA

    更新日期：1995-08-01 00:00:00

  • Fetal exsanguination and other vascular injuries from midtrimester genetic amniocentesis.

    abstract：:A case of fetal exsanguination after midtrimester genetic amniocentesis is reported. Examination of the ration of fetal-to-meternal red cells in the amniotic fluid obtained by amniocentesis for genetic study from 242 consecutive patients, including the reported case, suggests that fetal hemorrhage is relatively common...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(77)90812-2

    authors： Young PE,Matson MR,Jones OW

    更新日期：1977-09-01 00:00:00

  • Long-term effects of hormone replacement therapy on the uterus and on uterine circulation.

    abstract：OBJECTIVE:Our purpose was to study the effects of postmenopausal hormone replacement therapy on the uterus and uterine circulation. STUDY DESIGN:The study population consisted of 432 women, 58 to 59 years of age. Color Doppler ultrasonography with a transvaginal probe was used to measure the size of the uterus and the...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(93)90506-e

    authors： Pirhonen JP,Vuento MH,Mäkinen JI,Salmi TA

    更新日期：1993-02-01 00:00:00

  • Experience with the pulmonary artery catheter in obstetrics and gynecology.

    abstract：:Although traditionally the exclusive domain of other medical specialties, pulmonary artery catheterization may be of tremendous benefit to a variety of obstetric and gynecologic patients. Our experience with such invasive hemodynamic monitoring in 72 patients in an obstetrics and gynecology service is presented. In 86...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(85)80145-9

    authors： Clark SL,Horenstein JM,Phelan JP,Montag TW,Paul RH

    更新日期：1985-06-15 00:00:00

  • Vaginal cuff closure after minimally invasive hysterectomy: our experience and systematic review of the literature.

    abstract：OBJECTIVE:To determine the incidence of vaginal cuff dehiscence after minimally invasive hysterectomy, we reported our series of total laparoscopic hysterectomies with transvaginal colporraphy. STUDY DESIGN:We then conducted a systematic search of PubMed to retrieve published series of laparoscopic and robotic hystere...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ajog.2011.03.024

    authors： Uccella S,Ghezzi F,Mariani A,Cromi A,Bogani G,Serati M,Bolis P

    更新日期：2011-08-01 00:00:00

  • Benign gynecologic conditions among participants in the Breast Cancer Prevention Trial.

    abstract：OBJECTIVE:This study was undertaken to report on the benign gynecologic conditions occurring among women with an intact uterus at enrollment in the Breast Cancer Prevention Trial of the National Surgical Adjuvant Breast and Bowel Project. STUDY DESIGN:The incidence rates of several benign gynecologic conditions were d...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.ajog.2004.12.083

    authors： Chalas E,Costantino JP,Wickerham DL,Wolmark N,Lewis GC,Bergman C,Runowicz CD

    更新日期：2005-04-01 00:00:00

  • The effect of the prostaglandin F compounds on the contractility of the pregnant human uterus.

    abstract：:The aim of this paper was to study the influence of prostaglandins compounds on the pregnant human uterus in situ. Recordings of uterine motility were made on 14 pregnant women by measuring the amniotic pressure. PGE1 alpha and PGF2 alpha were administered intravenously as single injections. It was found that PGF2 ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(70)90042-6

    authors： Bygdeman M,Kwon SU,Mukherjee T,Roth-Brandel U,Wiqvist N

    更新日期：1970-02-15 00:00:00

  • Associations between 2 polymorphisms in the methylenetetrahydrofolate reductase gene and placental abruption.

    abstract：OBJECTIVE:Heritable thrombophilias have been implicated as a potential cause of abruption by vascular disruption at the uteroplacental interface. Polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) gene have been linked to vascular complications outside of pregnancy, which includes stroke. Given the underl...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.ajog.2007.06.046

    authors： Ananth CV,Peltier MR,De Marco C,Elsasser DA,Getahun D,Rozen R,Smulian JC,New Jersey-Placental Abruption Study Investigators.

    更新日期：2007-10-01 00:00:00

  • Relation between cyclic adenosine monophosphate and prostaglandin output by dispersed cells from human amnion and decidua.

    abstract：:We have examined the ability of activators of adenylate cyclase and cyclic adenosine monophosphate to affect the output of prostaglandins E and F by dispersed cells of amnion and decidua collected from women following spontaneous labor. Cyclic adenosine monophosphate production by amnion and decidua cells was stimulat...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(85)90592-7

    authors： Warrick C,Skinner K,Mitchell BF,Challis JR

    更新日期：1985-09-01 00:00:00

  • Defining the role of fluorescence in situ hybridization on uncultured amniocytes for prenatal diagnosis of aneuploidies.

    abstract：OBJECTIVE:This study examines the role of fluorescence in situ hybridization on uncultured amniocytes for prenatal diagnosis in a population at high risk for aneuploidies. STUDY DESIGN:All patients undergoing amniocentesis for fetal structural abnormality on ultrasonographic examination (performed from 13 to 39 weeks)...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(97)70600-8

    authors： D'Alton ME,Malone FD,Chelmow D,Ward BE,Bianchi DW

    更新日期：1997-04-01 00:00:00

  • First-trimester maternal serum alpha-fetoprotein screening for chromosome defects.

    abstract：:Low maternal serum alpha-fetoprotein values during the second trimester of pregnancy are associated with an increased risk of Down syndrome in the fetus. In this study a sensitive, monoclonal-based radioimmunoassay for alpha-fetoprotein was used to determine whether such an association also applies to the first trimes...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(88)90449-8

    authors： Milunsky A,Wands J,Brambati B,Bonacchi I,Currie K

    更新日期：1988-11-01 00:00:00

  • Use of video eyeglasses to decrease anxiety among children undergoing genital examinations.

    abstract：OBJECTIVE:Our purpose was to compare three techniques in their ability to decrease anxiety induced by the pelvic examination among children of different races. STUDY DESIGN:Eighty-nine subjects between 3 and 8 years old of white, African-American, and Hispanic race or ethnicity were randomly assigned to one of three d...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0002-9378(98)70341-2

    authors： Berenson AB,Wiemann CM,Rickert VI

    更新日期：1998-06-01 00:00:00

  • Prepubertal labial adhesions: evaluation of a referral population.

    abstract：OBJECTIVE:The purpose of this study was to assess patient demographics, clinical presentation, response to previous treatment, and current evaluation of prepubertal girls referred for gynecologic care of labial agglutination. STUDY DESIGN:A retrospective chart review of all prepubertal female patients with labial aggl...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1067/mob.2002.126201

    authors： Bacon JL

    更新日期：2002-08-01 00:00:00

  • Fetal evaluation by percutaneous blood sampling.

    abstract：:Percutaneous umbilical blood sampling (cordocentesis) provides direct access to the fetal circulation in the second and third trimesters of pregnancy. Seventeen patients underwent this procedure between December 1985 and December 1986 for evaluation of a variety of clinical situations, including nonlethal fetal abnorm...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(88)90795-8

    authors： Hogge WA,Thiagarajah S,Brenbridge AN,Harbert GM

    更新日期：1988-01-01 00:00:00

  • Clinical utility of saline solution infusion sonohysterography in a primary care obstetric-gynecologic practice.

    abstract：OBJECTIVE:The purpose of this study was to evaluate the role of saline solution infusion sonohysterography in clinical practice in patients with abnormal uterine bleeding. STUDY DESIGN:A prospective case-controlled study was conducted comparing two-dimensional transvaginal imaging with saline solution infusion sonohys...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(97)70351-x

    authors： Laughead MK,Stones LM

    更新日期：1997-06-01 00:00:00