Scanning electron microscopy of softened enamel.

Abstract:

:After exposing enamel specimens to 0.3% citric acid at pH 3.2 for various times, the acid was titrated to pH 7 before rinsing the specimens in water. After freeze-drying the specimens were examined by scanning electron microscopy. This procedure eliminates artefacts due to drying and mineral precipitation. The results showed that the outer region of softened enamel is much more delicate than previously thought, even after short (5- to 20-min) etching times. Mineral was lost from both prism boundaries and the prism bodies, resulting in a surface presenting thin, separate crystal bundles. In further studies, replicas of subsurface pores, created by resin impregnation, showed the softening depth to be several times greater than is suggested by techniques based on removing the softened enamel by physical forces. The results point to a need for improved methods of measuring softening depth. More importantly, it appears that the outer region of the softened layer remaining after an erosive challenge might be too fragile to resist frictional forces in vivo.

journal_name

Caries Res

journal_title

Caries research

authors

Eisenburger M,Shellis RP,Addy M

doi

10.1159/000073923

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

67-74

issue

1

eissn

0008-6568

issn

1421-976X

pii

73923

journal_volume

38

pub_type

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