Abstract:
:The study of plaque biofilms in the oral cavity is difficult as plaque removal inevitably disrupts biofilm integrity precluding kinetic studies involving the penetration of components and metabolism of substrates in situ. A method is described here in which plaque is formed in vivo under normal (or experimental) conditions using a collection device which can be removed from the mouth after a specified time without physical disturbance to the plaque biofilm, permitting site-specific analysis or exposure of the undisturbed plaque to experimental conditions in vitro. Microbiological analysis revealed plaque flora which was similar to that reported from many natural sources. Analytical data can be related to plaque volume rather than weight. Using this device, plaque fluoride concentrations have been shown to vary with plaque depth and in vitro short-term exposure to radiolabelled components may be carried out, permitting important conclusions to be drawn regarding the site-specific composition and dynamics of dental plaque.
journal_name
Caries Resjournal_title
Caries researchauthors
Robinson C,Kirkham J,Percival R,Shore RC,Bonass WA,Brookes SJ,Kusa L,Nakagaki H,Kato K,Nattress Bdoi
10.1159/000262398subject
Has Abstractpub_date
1997-01-01 00:00:00pages
194-200issue
3eissn
0008-6568issn
1421-976Xjournal_volume
31pub_type
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