Abstract:
:We have examined the migratory capacity of Schwann cells from the distal stump of a 1-week transected sciatic nerve of adult rat for a distance of 10 mm. The distal stump was introduced into the open end of a silicone chamber packed with artificial fibrin sponge (Gelaspon) soaked in phosphate-buffered saline (control chambers), cytosine arabinoside (Ara-C) (0.05 mM), or insulin (40 U/ml). Migrating Schwann cells were distinguished from fibroblasts by the presence of non-specific cholinesterase (nChE) activity and glial fibrillary acidic protein (GFAP). The cells of distal stumps including Schwann cells accepted Gelaspon as a suitable adhesive substratum. In the chambers filled with Gelaspon soaked in phosphate-buffered saline alone Schwann cells were outnumbered by fibroblasts. The addition of Ara-C resulted in greater numbers of Schwann cells, which migrate longer distances into the chambers. The application of insulin enhanced Schwann cell migration as well. These morphologic observations were further supported by biochemical measurements of nChE activity. The results suggest an influence on Schwann cell migration by fibroblasts of connective tissue sheaths and a stimulation of Schwann cell migration by insulin.
journal_name
Gliajournal_title
Gliaauthors
Dubový P,Svízenská Idoi
10.1002/glia.440060406subject
Has Abstractpub_date
1992-01-01 00:00:00pages
281-8issue
4eissn
0894-1491issn
1098-1136journal_volume
6pub_type
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