Abstract:
PURPOSE:To examine the heat sensitivity of DNA-dependent protein kinase (DNA-PK) activity in a variety of cultured mouse, hamster and human cell lines. MATERIALS AND METHODS:Eight cell lines, which have been routinely used in our laboratory, were examined. Cells were heated at 44.0 +/- 0.05 degrees C and DNA-PK activity was measured by a DNA-pull-down assay followed by gel-electrophoresis. Cellular sensitivity to hyperthermia and/or X-ray was evaluated by a colony formation assay. RESULTS:In mouse FSA1233 and FM3A cells, DNA-PK activity dropped to 15-16% of unheated control after 20 min of heating. In Chinese hamster V79 and CHO-K1 cells, kinase activity did not change appreciably after 20 min treatment but decreased to 60-70 and 22-23% after 40 or 60 min treatment, respectively. However, even after 180 min treatment, DNA-PK activity remained almost intact in human MOLT-4, MKN45 and A7 cells, and decreased only slightly in U937 cells. Hyperthermic radiosensitization was seen even in human cells but, as a trend, it was small compared with rodent cells. CONCLUSIONS:The heat sensitivity of DNA-PK was clearly different among mouse, hamster and human cells. The results suggested a possibility that the role of DNA-PK inactivation in hyperthermic radiosensitization might be variable, depending on cells, and would reinforce the warning that the direct extrapolation of data from rodent cells might lead to overestimation of the effectiveness of hyperthermia on human cancer.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Umeda N,Matsumoto Y,Yin HL,Tomita M,Enomoto A,Morita A,Mizukoshi T,Sakai K,Hosoi Y,Suzuki Ndoi
10.1080/09553000310001596959subject
Has Abstractpub_date
2003-08-01 00:00:00pages
671-80issue
8eissn
0955-3002issn
1362-3095pii
WQVR26P631FY1834journal_volume
79pub_type
杂志文章abstract:PURPOSE:Caffeine (1,3,7-trimethyl xanthine), a dietary component, has been shown to have widely varying effects on DNA damage induced by UV and ionizing radiation, depending upon pre- or post-irradiation administration and its concentration. Caffeine administered post-UV irradiation is known to inhibit enzymatic repair...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110034649
更新日期:2001-05-01 00:00:00
abstract::The form of the hazard function for radiation-induced pneumonitis death in mice was investigated. 'Hazard' refers to the instantaneous failure rate at a specified time, conditional upon non-failure to that time. Thus, the hazard function describes the time profile for the risk of pneumonitis death among still-survivin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552551
更新日期:1992-11-01 00:00:00
abstract:PURPOSE:Up to 90% of hereditary breast cancer cases are linked to germ-line mutations in one of the two copies of the BRCA1 or BRCA2 genes. Brca1 and Brca2 proteins are both involved in the cellular defence against DNA damage, although the precise function of the proteins is still not known. Some studies on a small num...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110097974
更新日期:2002-04-01 00:00:00
abstract::One-cell mouse embryos were irradiated with X-rays or neutrons. Analysis of the first, second, and third postradiation mitoses revealed that the yields of structural aberrations increased linearly after exposure to both radiation qualities. For X-rays the aberration frequency decreased from the first to the third mito...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551771
更新日期:1988-09-01 00:00:00
abstract:PURPOSE:To investigate possible variations in topoisomerase (topo) I and II activities and levels after X-ray treatment in the radiation repair proficient AA8 Chinese hamster cell line for comparison with the radiation sensitive mutant EM9. MATERIALS AND METHODS:AA8 and EM9 cells were irradiated with 5 Gy of X-rays an...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139809
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes. MATERIALS AND METHOD:Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co gamma-rays, 0.9 Gy 3H beta-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050201073
更新日期:2000-12-01 00:00:00
abstract:PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000801998875
更新日期:2008-05-01 00:00:00
abstract:PURPOSE:The LET position of the RBE maximum and its dependence on the cellular repair capacity was determined for carbon ions. Hamster cell lines of differing repair capacity were irradiated with monoenergetic carbon ions. RBE values for cell inactivation at different survival levels were determined and the differences...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139232
更新日期:1999-11-01 00:00:00
abstract::The aim was to review and summarize the results of 40 years of study concerning the response to ionizing radiation of the pair of L5178Y (LY) sublines, LY-R and LY-S, that differ in sensitivity to various DNA-damaging agents, among them X- and gamma-rays. The reviewed data indicate the key importance of DNA damage rep...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000500143534
更新日期:2005-05-01 00:00:00
abstract::I calculate the probability that a single high-energy ionizing particle or photon makes two widely spaced double-strand breaks in the same DNA molecule. Deletions (or inversions) between two breaks formed by the same incident particle are linear in radiation dose and occur even at extremely low dose-rates; deletions b...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144699
更新日期:1996-11-01 00:00:00
abstract:PURPOSE:A key enzyme of polyamine catabolism, spermidine/spermine N(1)-acetyltransferase (SSAT), is responsive to antiproliferative agents. The role of SSAT in cellular responses to X-ray irradiation was examined. MATERIALS AND METHODS:Exponentially growing HeLa S3 cells were irradiated by X-rays, and mRNA levels for ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000410001695886
更新日期:2004-05-01 00:00:00
abstract:PURPOSE:Zearalenone is a potent estrogenic metabolite produced by some Fusarium and Gibberella species. Therefore, the aim of this study was to determine the accumulation of Technetium-[99m]-labeled zearalenone in mice organs as well as to study the protective effect of Lactobacillus plantarum. MATERIALS AND METHODS:T...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1524990
更新日期:2018-12-01 00:00:00
abstract:PURPOSE:To summarize current knowledge regarding mechanisms of radiation-induced skin injury and medical countermeasures available to reduce its severity. Advances in radiation delivery using megavoltage and intensity modulated radiation therapy have permitted delivery of higher doses of radiation to well-defined tumor...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.3109/09553002.2013.765055
更新日期:2013-05-01 00:00:00
abstract::Purpose To investigate the effect of increasing the overall treatment time as well as delivering the compensating doses on the Balb/c breast adenocarcinoma (4T1) tumor. Materials and methods A total of 72 mice were divided into two aliquots (classes A and B) based on the initial size of their induced tumor. Each class...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2016.1117677
更新日期:2016-01-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.698366
更新日期:2012-08-01 00:00:00
abstract:PURPOSE:To compare experimental yields of single strand breaks (SSB) and double strand breaks (DSB) induced in plasmid DNA in aqueous solution by alpha-particles and Al(K) ultrasoft X-rays (USX) with the corresponding yields, generated via computer simulations, for a range of mean diffusion distances of the hydroxyl ra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110069308
更新日期:2001-10-01 00:00:00
abstract:PURPOSE:The purpose of this study was to assess the effect of a lavage solution containing methoxypolyethylene glycol 4-(3,4-dihydroxyphenethylamino)-4-oxobutanoate (MDO) for whole lung lavage (WLL) in dogs after the inhalation of depleted uranium (DU) dust at a dose of 30 mgUkg-1. MATERIALS AND METHODS:WLL was perfor...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1446228
更新日期:2018-05-01 00:00:00
abstract::It follows from the present work that the vascularization of the normal tissue present in the treatment volume limits the temperatures achieved during heat treatment of invasive ductal breast carcinoma. The temperatures can often be increased by giving fractionated heat treatment because heat treatment may reduce the ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114551821
更新日期:1991-07-01 00:00:00
abstract::Two novel nitroimidazole-carboranes were examined for their uptake and retention in two experimental murine solid tumours and in some normal tissues, using in vivo 11B magnetic resonance spectroscopy. The compounds were injected i.p. at 0.8mmol/kg into mice bearing either the SCCVII/Ha squamous cell carcinoma or KHT s...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144798
更新日期:1996-11-01 00:00:00
abstract::Bacterial infection of simple wounds was studied directly and quantitatively in adult mice given 6.5 Gy 60Co. Three days later, when neutropenia was evident, the skin and the medial gluteus muscle of anaesthetized mice were incised. A suspension of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae or Stre...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551671
更新日期:1990-08-01 00:00:00
abstract:PURPOSE:We examined the effects of manganese (III) meso-tetrakis (diethyl-2-5-imidazole) porphyrin, a metalloporphyrin antioxidant (MPA), on neural tissue radiation toxicity in vivo and on tumour cell radiosensitivity in vitro. MATERIALS AND METHODS:MPA was administered directly into the right lateral ventricle of you...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553000903419965
更新日期:2010-02-01 00:00:00
abstract:PURPOSE:To analyze the direct and transgenerational effects of exposure to low-dose 1 GHz (mobile phone/wireless telecommunication range) and 10 GHz (radar/satellite communication range) radiofrequency electromagnetic fields (RF-EMF) on the motility of ciliates Spirostomum ambiguum. MATERIALS AND METHODS:S. ambiguum w...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.868612
更新日期:2014-03-01 00:00:00
abstract::Radiation survival curves have been obtained for crypts in the small and large intestine of male BDF1 mice using the microcolony assay. Four regions of the small intestine and three regions of the large intestine were studied. One of the major objectives was to determine the optimal conditions for the microcolony assa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097144265
更新日期:1997-02-01 00:00:00
abstract:PURPOSE:To examine whether a simulated mobile telephone transmission at 915 MHz has an effect on cognitive function in man. MATERIALS AND METHODS:Thirty-six subjects in two groups were each given two training sessions and then three test sessions in a randomized three-way cross-over design. About 1 W mean power at 915...
journal_title:International journal of radiation biology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/095530099140375
更新日期:1999-04-01 00:00:00
abstract:PURPOSE:The long-term effect of low and moderate doses of ionizing radiation on the lens is still a matter of debate and needs to be evaluated in more detail. MATERIAL AND METHODS:We conducted a detailed histological analysis of eyes from B6C3F1 mice cohorts after acute gamma irradiation (60Co source; 0.063 Gy/min) at...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1876951
更新日期:2021-01-19 00:00:00
abstract:PURPOSE:The actinides are chemical poisons and radiological hazards. One challenge to better appraise their toxicity and develop countermeasures in case of exposure of living organisms is to better assess pathways of contamination. Because of the high chemical affinity of those actinide elements for phosphate groups an...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.886795
更新日期:2014-11-01 00:00:00
abstract:PURPOSE:Epidemiological studies have demonstrated a possible correlation between exposure to extremely low-frequency magnetic fields (ELF-MF) and cancer. However, this correlation has yet to be definitively confirmed by epidemiological studies. The principal objective of this study was to assess the effects of 60 Hz ma...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.652724
更新日期:2012-04-01 00:00:00
abstract:PURPOSE:Since previous research found an increase in the rate of aneuploidies in human lymphocytes exposed to radiofrequencies, it seems important to perform further studies. The objective of this study was then to investigate whether the exposure to RF (radiofrequency) radiation similar to that emitted by mobile phone...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.542543
更新日期:2011-04-01 00:00:00
abstract:PURPOSE:Previous systematic structure-activity studies of the desferrithiocin (DFT) platform have allowed the design and synthesis of analogues and derivatives of DFT that retain the exceptional iron-clearing activity of the parent, while eliminating its adverse effects. We hypothesized that a similar approach could be...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000902781089
更新日期:2009-04-01 00:00:00
abstract::CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of 3H-thymidine prelab...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551361
更新日期:1993-08-01 00:00:00