Abstract：
PURPOSE:Up to 90% of hereditary breast cancer cases are linked to germ-line mutations in one of the two copies of the BRCA1 or BRCA2 genes. Brca1 and Brca2 proteins are both involved in the cellular defence against DNA damage, although the precise function of the proteins is still not known. Some studies on a small number of samples as well as the present pilot study also suggested that BRCA1 heterozygosity may lead to impaired repair of ionizing-radiation-induced DNA double-strand breaks. The purpose of the study was to test in a larger family-matched study whether carriers of BRCA1 or BRCA2 mutations have an increased sensitivity to ionizing radiation. MATERIALS AND METHODS:In a blind study, the effect of different germ-line mutations in one allele of the BRCA1 or BRCA2 gene on the ability to repair X-ray-induced DNA breaks was investigated. Fibroblasts and lymphocytes were taken from heterozygotic individuals (BRCA1+ /- and BRCA2+ /-) with different mutations and from relatives proven to be non-carriers of the BRCA mutations. Rejoining of DNA breaks was analysed by pulsed-field gel electrophoresis (for fibroblasts) or the comet assay (for lymphocytes). RESULTS:Significant interindividual differences were found in the capacities of the fibroblasts and lymphocytes to rejoin DNA breaks induced by X-radiation. However, these differences were not related to heterozygosity in BRCA1 or BRCA2. CONCLUSIONS:Cells from carriers of mutations in one allele of the BRCA1 or BRCA2 genes have no gross defects in their ability to rejoin radiation-induced DNA breaks. Hence, these carriers may not be at risk of developing excess normal tissue reactions after radiotherapy consistent with data from recent clinical studies.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Nieuwenhuis B,Van Assen-Bolt AJ,Van Waarde-Verhagen MA,Sijmons RH,Van der Hout AH,Bauch T,Streffer C,Kampinga HHdoi
10.1080/09553000110097974subject
Has Abstractpub_date
2002-04-01 00:00:00pages
285-95issue
4eissn
0955-3002issn
1362-3095journal_volume
78pub_type
杂志文章abstract：PURPOSE:Cartilage degradation usually results as a consequence of inflammatory processes in the joints. To study this phenomenon experimentally, adjuvant-induced arthritis (AIA) was used as a model of chronic inflammation under the influence of irradiation. The potential chondroprotective effect of 13% aqueous extract ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.530337
更新日期：2011-03-01 00:00:00
abstract：PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000701570196
更新日期：2007-11-01 00:00:00
abstract：:A/J and C57BL/6J mice, strains which differ in susceptibility to chemical-induced lung tumours, were examined for differential sensitivity to radon-induced lung cancer. Sixty weeks after exposure to 952 WLM radon (3.34 J h1 m-3), no lung tumours were found in the control, sham- (one dust-) exposed, or radon-exposed C5...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097144184
更新日期：1997-03-01 00:00:00
abstract：PURPOSE:The effects of radiation +/- hypogravity on immunologic function were investigated using the Partial Weight Suspension (PWS) model ( Wagner et al. 2010 ). MATERIALS AND METHODS:Mice were exposed to 0.5, 1, or 2 Gray (Gy) dose of gamma radiation and then placed in the PWS system for 4, 24, 48 hours, or 4 days. ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.595875
更新日期：2011-10-01 00:00:00
abstract：PURPOSE:This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after γ-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. METHODS:MSC were irradiated with 2 and 20 Gy by (60)Co. For ATM inhibition, specific inhibitor KU5593...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.775530
更新日期：2013-07-01 00:00:00
abstract：:The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145779
更新日期：1996-04-01 00:00:00
abstract：PURPOSE:To follow plasma Flt3-ligand (FL) concentrations in irradiated animals in order to evaluate it as an indicator of bone marrow damage for the management of accidental radiation-induced aplasia. MATERIALS AND METHODS:Non-human primates were irradiated at doses ranging from 2 to 8 Gy, using whole- or partial-body...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110043711
更新日期：2001-06-01 00:00:00
abstract：PURPOSE:To understand the mechanisms of life-shortening due to early neoplastic death caused by chronic low dose-rate (LDR; 20 mGy/22 h/day) radiation which accumulates to a high dose (HD; 8 Gy) (LDR/HD) as reported previously. MATERIALS AND METHODS:Female B6C3F(1) mice were continuously exposed to LDR/HD gamma-rays u...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.545861
更新日期：2011-07-01 00:00:00
abstract：:Eelpout (Zoarces viviparus), a marine fish, were exposed to gamma-radiation at a dose-rate of 2.0 mGy h-1. They were injected with the antigen trinitrophenyl-keyhole limpet haemocyanin (TNP-KLH) and their specific humoral immune response to the TNP hapten measured. There were no differences in the primary antibody res...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550091
更新日期：1995-01-01 00:00:00
abstract：PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was esti...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138745
更新日期：2000-03-01 00:00:00
abstract：:Purpose: To examine the effects of low-dose exposure to uranium with a systems biology approach, a multiscale high-throughput multi-omics analysis was applied with a protocol for chronic exposure to the rat kidney. Methods: Male and female rats were contaminated for nine months through their drinking water with a nont...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1577567
更新日期：2019-06-01 00:00:00
abstract：PURPOSE:Hypoxic cells in tumours are resistant to 5-fluorouracil (5-FU). This in vivo study investigated the ability of hypoxia to regulate the gene expression of thymidylate synthase (TS), the target enzyme of 5-FU. MATERIALS AND METHODS:C3H mammary carcinomas, grown in the feet of female CDF1 mice, were used for all...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139944
更新日期：1999-07-01 00:00:00
abstract：PURPOSE:Angiotensin-converting enzyme (ACE) inhibitors are effective in the prophylaxis of radiation-induced renal and lung injury. Studies were designed to determine whether blocking the angiotensin II (AII) receptor, rather than blocking AII synthesis with ACE inhibitors, would also be effective. MATERIALS AND METHO...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098142257
更新日期：1998-04-01 00:00:00
abstract：:Purpose: Cancer is one of the most common causes of mortality and morbidity worldwide. Vaccines have been emerged as an attractive approach for their capacity of eliciting long-term immune response targeting cancer cells. Attenuated avirulent Toxoplasma gondii stimulate immunity and activate antitumor cells thereby el...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1739772
更新日期：2020-06-01 00:00:00
abstract：PURPOSE:The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. MATERIALS AND METHODS:Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometr...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.901579
更新日期：2014-06-01 00:00:00
abstract：:CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of 3H-thymidine prelab...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551361
更新日期：1993-08-01 00:00:00
abstract：PURPOSE:Ifosfamide as a chemotherapeutic drug is used for the treatment of different cancer types. The purpose of this study is the preparation of 99mTc-ifosfamide complex to be evaluated as a potential candidate for tumor imaging. MATERIALS AND METHODS:The radiolabeling of ifosfamide with technetium-99m was carried o...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1524945
更新日期：2018-12-01 00:00:00
abstract：:We used the 'comet assay' to compare the amount of radiation-induced DNA damage in three tumour cell lines (MeWo, PECA 4451 and PECA 4197) and the extent of DNA repair in two of these lines (MeWo and PECA 4197). Tumour cells were irradiated with X-rays (0.1-10 Gy), embedded in agarose on slides, lysed with sodium dode...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550371
更新日期：1994-03-01 00:00:00
abstract：:Mouse C3H 10T1/2 cells are most sensitive to radiation-induced neoplastic transformation in the G2/M-phase of the cell cycle. When synchronized 10T1/2 cells were exposed to phorbol 12-myristate 13-acetate (TPA) after irradiation, transformation of cells not in the transformation-sensitive window was enhanced, but tran...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551971
更新日期：1993-12-01 00:00:00
abstract：:Radiation-induced DNA double-strand breaks (dsb) were studied in Ehrlich ascites tumour cells (EATC) by sedimentation in neutral sucrose gradients at low centrifuge speed. Dsb induction was found to be linear with dose with a frequency of: ndsbmr-1D-1 = (11.7 +/- 2) x 10(-12)Gy-1 for 140 kV X-rays and ndsbmr-1D-1 = (1...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814552201
更新日期：1988-11-01 00:00:00
abstract：PURPOSE:To investigate the mechanism of NFkappaB activation by X-rays in normal primary rat astrocytes. MATERIALS AND METHODS:Primary cultures of type I astrocytes generated from the cortex of neonatal rats were exposed to X-rays with and without various kinase inhibitors and a protease inhibitor. The nuclear or cytop...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141195
更新日期：1998-11-01 00:00:00
abstract：PURPOSE:To examine the relationship between cell killing and residual chromatin breaks after irradiation with qualitatively different types of radiation in six human cell lines. MATERIALS AND METHODS:Six human tumour cell lines and normal human cells were irradiated with 200 kV X-rays and two carbon-ion beams accelera...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050134429
更新日期：2000-09-01 00:00:00
abstract：:Samples of human rib were collected at autopsy and 20 were selected for marrow fat cell measurement, representing an age range of 16-96 years. The mean diameter of fat cells in red bone marrow of human rib was found to increase from around 48 microns at ages 16-29 years to around 65 microns at ages 82-96 years. There ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551681
更新日期：1995-12-01 00:00:00
abstract：PURPOSE:5-Formyluracil (5-foU) is a major derivative of thymine produced in DNA by ionizing radiation and various chemical oxidants. It has been previously shown that 5-foU in template DNA directs misincorporation of nucleotides by DNA polymerases during in vitro DNA synthesis. The present experiments were designed to ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530001453113
更新日期：2001-01-01 00:00:00
abstract：:Radiation-induced myocardial degeneration in the rat is preceded by changes in capillary structure and function, which may be a major factor in the pathogenesis of radiation-induced heart disease. In order to investigate the mechanism of capillary damage we studied endothelial cell proliferation in untreated control r...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551131
更新日期：1990-05-01 00:00:00
abstract：PURPOSE:To evaluate the impact of oral administration of purslane (Portulaca oleracea) extract or fish oil and their co-treatments in the modulation of radiation-induced damage. MATERIAL AND METHODS:Purslane (P) (400 mg/kg body weight) or fish oil (Fo) (60 mg/kg body weight) was administrated to male albino rats via g...
journal_title：International journal of radiation biology
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doi：10.3109/09553002.2014.926040
更新日期：2014-12-01 00:00:00
abstract：PURPOSE:To evaluate the radioprotective effect of dieckol, a component of the brown algae Ecklonia cava. MATERIALS AND METHODS:Mice were lethally irradiated, treated with dieckol and surveyed for survival and the mechanism of radioprotection. RESULTS:Dieckol treatment lengthened the survival of irradiated mice and si...
journal_title：International journal of radiation biology
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doi：10.3109/09553002.2010.487011
更新日期：2010-10-01 00:00:00
abstract：PURPOSE:Detection of gene targets applicable to biological dosimetry and early detection of radiation-induced cell damage. MATERIALS AND METHODS:MCF-7 human mammary carcinoma cells were exposed to 2 and 6 Gy X-rays. Expression of 1176 genes was traced with the Atlas Human 1.2 Array (Clontech). RESULTS:(1) Based on da...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/0955300032000093146
更新日期：2003-05-01 00:00:00
abstract：PURPOSE:To estimate gamma-ray doses received by Hiroshima atomic bomb survivors using electron spin resonance (ESR) of tooth enamel and to compare the results with cytogenetic data. MATERIALS AND METHODS:Tooth enamel ESR was performed for 100 teeth donated from 69 Hiroshima survivors, and conventional cytogenetic exam...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141870
更新日期：1998-06-01 00:00:00
abstract：:Radiation survival curves have been obtained for crypts in the small and large intestine of male BDF1 mice using the microcolony assay. Four regions of the small intestine and three regions of the large intestine were studied. One of the major objectives was to determine the optimal conditions for the microcolony assa...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097144265
更新日期：1997-02-01 00:00:00
