Abstract:
PURPOSE:A key enzyme of polyamine catabolism, spermidine/spermine N(1)-acetyltransferase (SSAT), is responsive to antiproliferative agents. The role of SSAT in cellular responses to X-ray irradiation was examined. MATERIALS AND METHODS:Exponentially growing HeLa S3 cells were irradiated by X-rays, and mRNA levels for SSAT were measured as a function of post-irradiation time through Northern hybridization. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect alternatively spliced SSAT mRNAs. The intracellular polyamine content was measured by the o-phthalaldehyde method and the enzymatic activity of SSAT by the increased amount of acetylated spermidine after incubation. RESULTS:Not only SSAT mRNA, but also an alternatively spliced mRNA accumulated at the initial stage of growth inhibition after the first or second replication of irradiated cells. The maximum fold increase relative to the level of non-irradiated cells was 3.0-3.5 for both transcripts after 5-Gy irradiation. On the other hand, the mRNA of ornithine decarboxylase, a key enzyme of polyamine synthesis, was little influenced by X-ray treatment. Enzymatic activity of SSAT and the acetylspermidine level were elevated after X-ray irradiation. CONCLUSIONS:Activation of SSAT and the induction of alternatively spliced mRNA of the SSAT gene play an important role in regulating growth inhibition and cell death after X-ray irradiation.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Mita K,Fukuchi K,Hamana K,Ichimura S,Nenoi Mdoi
10.1080/09553000410001695886subject
Has Abstractpub_date
2004-05-01 00:00:00pages
369-75issue
5eissn
0955-3002issn
1362-3095pii
1TVRCM0XA6VW5MQYjournal_volume
80pub_type
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journal_title:International journal of radiation biology
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