Abstract:
:A 4-day colorimetric tetrazolium dye (MTT) assay was used to assess the cytotoxicity of adriamycin (ADM), vincristine (VCR), and idarubicin (IDA) in blasts isolated from 37 patients with newly diagnosed and pretreated acute myeloid leukemia (AML). The effect of verapamil (VRP) as a chemosensitizer was studied in relation to the expression of the membrane efflux pump P-glycoprotein (PGP) as determined by a semiquantitative flow-cytometric proceder. A slight positive correlation was found between the fraction of cells expressing PGP and the ID50 values for ADM and VCR, but not between cellular PGP content and sensitivity to IDA. The overall data showed no significant sensitization effect of VRP. However, in specimens with more than 10% cells expressing PGP, 2 microM VRP sensitized cells to ADM and VCR significantly. The median of sensitization ratios (SRs), i.e., the ratios of cytotoxic drug ID50 in the absence/presence of VRP, were 1.89 and 2.0, respectively. No sensitizing effect of VRP on the cytotoxicity of IDA was observed. Related to the clinical status, the median fraction of PGP-positive blasts was elevated fourfold in pretreated patients (n = 16) in comparison to patients with de novo AML (n = 19). No differences in ID50 values were observed between newly diagnosed and pretreated patients. However, SRs for ADM and VCR were higher in samples of pretreated patients compared with de novo AML. PGP-mediated cellular drug resistance may thus be circumvented in leukemic blasts by application of chemosensitizers or, potentially, alternative anthracyclines.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Müller MR,Lennartz K,Boogen C,Nowrousian MR,Rajewsky MF,Seeber Sdoi
10.1007/BF01703946subject
Has Abstractpub_date
1992-11-01 00:00:00pages
206-12issue
5eissn
0939-5555issn
1432-0584journal_volume
65pub_type
杂志文章abstract::Hemoglobin (Hb) Korle-Bu (beta73; Asp-Asn) is the most frequent of the rare beta-chain variants in the population of West Africa whereas Hb E (beta26; Glu-Lys) is common among the Southeast Asian population. We report a hitherto undescribed condition in which these two beta-chain variants co-segregate. The proband was...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-002-0485-0
更新日期:2002-07-01 00:00:00
abstract::We tested two Fc gamma receptor I (Fc gamma RI); six Fc gamma RII; and six Fc gamma RIII-specific monoclonal antibodies (mAb) for their capacity to inhibit monocyte and polymorphonuclear granulocyte (PMN) immune phagocytosis which is mediated by Fc gamma R. We used human red blood cells (rbc) coated with hIgG1 or mIgG...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050249
更新日期:1997-01-01 00:00:00
abstract::There is little information about cardiovascular adverse event (CV-AE) incidence in chronic myeloid leukemia (CML) patients treated with bosutinib in the real-life practice. We identified 54 consecutive CML patients treated with bosutinib, stratified according to the Systematic Coronary Risk Evaluation (SCORE) assessm...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-019-03705-y
更新日期:2019-08-01 00:00:00
abstract::The clinical targets for which new generations of antithrombotics have been or are currently under clinical development are those associated with a high risk for thromboembolism, (a) patients undergoing general, orthopedic, major abdominal, and cancer surgery, to prevent venous thromboembolism; (b) patients with deep-...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s002770050158
更新日期:1996-04-01 00:00:00
abstract::Sickle cell patients are characterized by stress erythropoiesis involving cytokines, growth factors, and adhesion molecules. We set out to determine whether serum soluble vascular cell adhesion molecule-1 (sVCAM-1) levels, which are inversely related to red blood cell counts in sickle cell disease (SCD), reflect eryth...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-003-0610-8
更新日期:2003-03-01 00:00:00
abstract::Interferon alpha (IFN) inhibits the growth of the abnormal clone in patients with myeloproliferative disorders, leading to a reduction of the clinical and laboratory signs of the pathologic myeloproliferation. The therapeutic efficacy of IFN in polycythemia vera (PV) is demonstrated by the summarized treatment results...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s002770050563
更新日期:2000-03-01 00:00:00
abstract::While much has been learned about the basic immunology and clinical characteristics of immune thrombocytopenia, many important questions remain with regard to pathogenesis, disease progression, identification of novel therapeutic targets and approaches, and clinical trials that rationalize and optimize use of existing...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-010-0917-1
更新日期:2010-07-01 00:00:00
abstract::Multicentric Castleman's disease (MCD) is a rare systemic lymphoproliferative disorder with too few patient series reported in the literature to have a clear idea about the etiology, outcome and the best treatment available. Systemic reactive amyloidosis is a very rare complication of MCD and its presence worsens the ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-003-0718-x
更新日期:2003-12-01 00:00:00
abstract::Autoantibody against erythrocytes has occasionally been observed in patients with de novo acute myelocytic leukemia (AML). However, it is not clear whether this autoantibody in AML patients induces frank hemolysis (autoimmune hemolytic anemia, AIHA), as seen in lymphoid neoplasms. We present two de novo AML patients w...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF00663016
更新日期:1996-01-01 00:00:00
abstract::High-dose immunosuppressive therapy (HDIT) with autologous hematopoietic stem cell transplantation (AHSCT) is a promising approach to treatment of multiple sclerosis (MS) patients. In this paper, we present the long-term outcomes of a prospective single-center study with the analysis of the safety and efficacy of HDIT...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-015-2337-8
更新日期:2015-07-01 00:00:00
abstract::As ethnic variations are known to exist in inherited genetic defects, the clinico-haematological profile of Indian children with thrombophilia may be different from that of Caucasians. The aim of the study was to analyse the phenotypic and genotypic causes of thrombophilia in Indian children. Forty patients with arter...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-004-0952-x
更新日期:2005-02-01 00:00:00
abstract::Uptake and biochemical and biological effects of antisense oligodeoxynucleotides (ODN) specific for c-abl and bcr genes were studied in normal immature myeloid cells. CD34-positive cells were purified by positive and negative selection and cultured in liquid culture for 7 days. These cells were then incubated with ODN...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF00641311
更新日期:1996-02-01 00:00:00
abstract::In this prospective multicentric study, we investigated the contribution of positron emission tomography (PET) scanning to the staging of Hodgkin's lymphoma (HL) by computed tomography (CT) and attempted to determine whether it has any impact on therapeutic approach. One hundred eighty six consecutive patients with HL...
journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00277-007-0356-9
更新日期:2007-12-01 00:00:00
abstract::Multiple myeloma is a malignant B-cell neoplasm that involves the skeleton in approximately 80% of the patients. With an average age of 60 years and a 5-years survival of nearly 45% Brenner et al. (Blood 111:2516-2520,35) the onset is to be classified as occurring still early in life while the disease can be very aggr...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-009-0829-0
更新日期:2009-12-01 00:00:00
abstract::To determine the value of aerosol amphotericin B inhalations for prevention of invasive pulmonary aspergillosis (IPA), we initiated a prospective randomized multicenter trial. The scheduled intent-to-treat interim analysis included 115 patients (30%) with prolonged neutropenia after chemotherapy for acute myeloid leuk...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1007/BF01697981
更新日期:1995-12-01 00:00:00
abstract::The prognostic impact of extramedullary plasmacytomas (EMPs) on newly diagnosed symptomatic multiple myeloma (MM) was evaluated in the context of treatment approach including autologous stem cell transplantation (ASCT) and chemotherapy alone. A total of 275 consecutive patients with newly diagnosed MM were included, a...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2216-8
更新日期:2015-03-01 00:00:00
abstract::Anthracyclines are a major component in the therapy of non-Hodgkin's lymphoma. However, due to their cardiac toxicity potential, curative and palliative treatment is often limited in patients with preexisting cardiac dysfunction. Liposomal doxorubicin formulations have been described to be less cardiotoxic than conven...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-011-1308-y
更新日期:2012-03-01 00:00:00
abstract::Gastric mucosa-associated lymphoid tissue (MALT) lymphoma and diffuse large B cell lymphoma (DLBCL) show a spectrum of disease characterized by varying proportions of low-grade and high-grade components. While the natural history and optimum treatment for low-grade gastric MALT lymphoma and DLBCL is well established, ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-008-0604-7
更新日期:2009-05-01 00:00:00
abstract::Myelodysplastic syndromes (MDS) are hematopoietic stem cell malignancies associated with an erythroid maturation defect, resulting in anemia. Treatments for MDS include erythropoiesis-stimulating agents (ESAs). The identification of prognostic markers is important to help predict response and improve outcomes. Various...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-019-03799-4
更新日期:2020-01-01 00:00:00
abstract::Increasing the number of megakaryocytic cells in stem cell transplants by ex vivo expansion culture may provide an approach to accelerate platelet engraftment after high-dose chemotherapy. However, it is unknown if a relationship exists between the expansion potential of progenitor cells and the time to platelet engra...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-002-0443-x
更新日期:2002-04-01 00:00:00
abstract::The efficacy of a single dose of MCNU 150 mg was evaluated in nine symptomatic patients with essential thrombocythemia (ET). As the platelet counts increased during the following 4 weeks, an extra dose of 100 mg of MCNU was administered to three patients. All patients had thrombotic or hemorrhagic complications. Seven...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01738474
更新日期:1993-05-01 00:00:00
abstract::Venous thromboembolism (VTE), including deep venous thrombosis (DVT) and pulmonary embolism (PE), occurs secondary to a number of hereditary and acquired disorders of hemostasis. A recently recognized polymorphism in Factor V (FV) gene H1299R (also named HR2) has been reported to be a possible risk factor for the deve...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-008-0543-3
更新日期:2008-12-01 00:00:00
abstract::Relapse remains one of the major obstacles in Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ALL) even after allogeneic hematopoietic stem cell transplantation. The persistence of leukemia-propagating cells (LPCs) may lead to the recurrence of Ph+ALL. Using a xenograft assay, LPCs enrichment in the ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-018-3253-5
更新日期:2018-05-01 00:00:00
abstract::A case of congenital leukemia with monosomy 7 is reported. Immunological study of the blast cells using monoclonal antibodies was suggestive of both myelomegakaryocytic and T-lymphoblastic leukemia. Chromosomal analysis of the bone marrow cells showed monosomy 7. Chemotherapy was initiated with a combination of adriam...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01836073
更新日期:1992-12-01 00:00:00
abstract::It is often difficult for standard blood banks in Korea to supply adequate amounts of blood for patients with rare phenotype. Moreover, the definition of a blood in need is ambiguous, and much remains to be learned. In this study, we determined the prevalence of various red blood cell (RBC) antigens from a donor viewp...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-016-2645-7
更新日期:2016-05-01 00:00:00
abstract::The aim of this study was to evaluate the role of computed tomography (CT)-guided core needle biopsy (CNB) performed by modified coaxial technique as an alternative tool to surgical biopsy in patients with refractory or recurrent lymphomas. Between May 2005 and May 2012, 57 CT-guided CNB of deep lesions were performed...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2078-0
更新日期:2014-09-01 00:00:00
abstract::Extranodal natural killer/T cell lymphoma, nasal type (ENKTL) is an aggressive disease with a poor prognosis, requiring risk stratification in affected patients. We designed a new prognostic model specifically for ENKTL to identify high-risk patients who need more aggressive therapy. We retrospectively reviewed 158 pa...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2089-x
更新日期:2014-09-01 00:00:00
abstract::Amifostine increases in vitro burst-forming unit-erythroid and colony-forming unit-granulocyte/granulcoyte-macrophage cultured from bone-marrow cells from patients with myelodysplastic syndrome (MDS). Several small clinical studies give divergent informations about the potential of amifostine as single agent to improv...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050589
更新日期:2000-05-01 00:00:00
abstract::Since July 2017, different generic imatinib formulations have been introduced in Italy for the treatment of patients with chronic myeloid leukemia (CML). We analyzed 168 chronic phase CML patients treated with branded imatinib for a median of 12 years (range 1-16) at a single institution who switched to a single gener...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-020-04096-1
更新日期:2020-12-01 00:00:00
abstract::The prognostic value of cytogenetics in adult acute lymphoblastic leukemia (ALL) is not as established as in childhood ALL. We have analyzed the outcome and prognostic value of karyotype in 84 adults diagnosed with Philadelphia-negative ALL from a single institution that received induction chemotherapy and had success...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-011-1331-z
更新日期:2012-01-01 00:00:00