Abstract:
:Keratinocytes undergo a dramatic phenotypic conversion during reepithelialization of skin wounds to become hyperproliferative, migratory, and invasive. This transient healing response phenotypically resembles malignant transformation of keratinocytes during squamous cell carcinoma progression. Here we present the first analysis of global changes in keratinocyte gene expression during skin wound healing in vivo, and compare these changes to changes in gene expression during malignant conversion of keratinized epithelium. Laser capture microdissection was used to isolate RNA from wound keratinocytes from incisional mouse skin wounds and adjacent normal skin keratinocytes. Changes in gene expression were determined by comparative cDNA array analyses, and the approach was validated by in situ hybridization. The analyses identified 48 candidate genes not previously associated with wound reepithelialization. Furthermore, the analyses revealed that the phenotypic resemblance of wound keratinocytes to squamous cell carcinoma is mimicked at the level of gene expression, but notable differences between the two tissue-remodeling processes were also observed. The combination of laser capture microdissection and cDNA array analysis provides a powerful new tool to unravel the complex changes in gene expression that underlie physiological and pathological remodeling of keratinized epithelium.
journal_name
Oncogenejournal_title
Oncogeneauthors
Pedersen TX,Leethanakul C,Patel V,Mitola D,Lund LR,Danø K,Johnsen M,Gutkind JS,Bugge THdoi
10.1038/sj.onc.1206614subject
Has Abstractpub_date
2003-06-19 00:00:00pages
3964-76issue
25eissn
0950-9232issn
1476-5594pii
1206614journal_volume
22pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::The mechanism by which Bcl-2 inhibits apoptosis is unknown. The Bcl-2 protein is localized to intracellular membranes, including the endoplasmic reticulum (ER). The ER is the major intracellular reservoir of Ca2+ in non-muscle cells, sequestering Ca2+ for use in intracellular signaling, and is a prime target of oxidat...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-05-16 00:00:00
abstract::EZH2 (enhancer of zeste homolog 2) is a critical enzymatic subunit of the polycomb repressive complex 2 (PRC2), which trimethylates histone H3 (H3K27) to mediate gene repression. Somatic mutations, overexpression and hyperactivation of EZH2 have been implicated in the pathogenesis of several forms of cancer. In partic...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.571
更新日期:2015-01-22 00:00:00
abstract::Leukemic lymphoblasts within different immunophenotypic populations possess stem cell properties. However, whether or not the self-renewal program is retained from stem cells or conferred on progenitors by leukemogenic molecules remains unknown. We have addressed the issue in the context of TEL-AML1-associated acute l...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.148
更新日期:2015-04-16 00:00:00
abstract::Proteinase-activated receptor 2 (PAR2) is a G protein-coupled receptor that is activated by trypsin-like proteinases. PAR2 is detected in breast tumor specimens; however, it is not clear how PAR2 level in breast cancer cell/tissues compares with normal cell/tissues. Here, we show the elevation of PAR2 protein level in...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.163
更新日期:2009-08-27 00:00:00
abstract::Cancer cells are known to adopt aerobic glycolysis in order to fuel tumor growth, but the molecular basis of this metabolic shift remains largely undefined. O-GlcNAcase (OGA) is an enzyme harboring O-linked β-N-acetylglucosamine (O-GlcNAc) hydrolase and cryptic lysine acetyltransferase activities. Here, we report that...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0975-3
更新日期:2020-01-01 00:00:00
abstract::Germline BAP1 mutations predispose to several cancers, in particular malignant mesothelioma. Mesothelioma is an aggressive malignancy generally associated with professional exposure to asbestos. However, to date, we found that none of the mesothelioma patients carrying germline BAP1 mutations were professionally expos...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.243
更新日期:2016-04-14 00:00:00
abstract::Gene expression patterns in normal and v-myc-transformed quail embryo fibroblasts were compared by mRNA differential display. Displaying approximately 2500 mRNA species by reverse transcription/PCR, reamplification of 73 differential cDNA fragments and rescreening by Northern analysis led to the isolation of a clone, ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1200930
更新日期:1997-03-06 00:00:00
abstract::To gain a more complete understanding of c-myc regulation in chickens, we have completed the structural characterization of the chicken c-myc gene and have begun to investigate c-myc transcription and protein expression. A comparison of c-myc structure and expression between mammals and birds presents an enigma: there...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-06-20 00:00:00
abstract::wnt genes encode secretory glycoproteins that have been implicated in growth control and development in mice, frogs and insects. In this report we examine properties of two wnt genes recently identified in the nematode Caenorhabditis elegans. The first gene, Ce-wnt-1, was previously identified by a polymerase chain re...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Previously, we cloned two gene fragments encoding novel protein tyrosine phosphatases, termed PTP-U1 and PTP-U2. Here, we report the full-length sequence, expression, and chromosomal localization of the PTP-U2 gene. The cDNA for PTP-U2, which was obtained from a human normal kidney library, predicts a protein of 1216 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-05-04 00:00:00
abstract::Augmented reactive oxygen species levels consequential to functional alteration of key mitochondrial attributes contribute to carcinogenesis, either directly via oxidative DNA damage infliction or indirectly via activation of oncogenic signaling cascades. We previously reported activation of a key oncogenic signaling ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.346
更新日期:2017-03-30 00:00:00
abstract::The aspartic protease cathepsin D (cath-D) is a key mediator of induced-apoptosis and its proteolytic activity has been generally involved in this event. During apoptosis, cath-D is translocated to the cytosol. Because cath-D is one of the lysosomal enzymes that requires a more acidic pH to be proteolytically active r...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209221
更新日期:2006-03-23 00:00:00
abstract::Caspases are a family of cysteine proteases expressed as inactive zymogens in virtually all animal cells. These enzymes play a central role in most cell death pathways leading to apoptosis but growing evidences implicate caspases also in nonapoptotic functions. Several of these enzymes, activated in molecular platform...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1208524
更新日期:2005-08-04 00:00:00
abstract::Medulloblastoma (MB) is the most common malignant pediatric brain tumor. Since surviving patients experience severe neurocognitive disabilities, better and more effective treatments are needed to enhance their quality of life. Casein kinase 2 (CK2) is known to regulate cell growth and survival in multiple cancers; how...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0927-y
更新日期:2019-10-01 00:00:00
abstract::Cyclooxygenase-2 (COX-2) is an inducible enzyme that contributes to the generation of chronic inflammation in response to chemical carcinogens and environmental stresses, including ultraviolet B (UVB) irradiation. Although post-translational histone modifications are believed to have an important role in modulating tr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.71
更新日期:2013-01-24 00:00:00
abstract::Topoisomerase IIalpha (topoIIalpha) is an essential mammalian enzyme that topologically modifies DNA and is required for chromosome segregation during mitosis. Previous research suggests that inhibition of topoII decatenatory activity triggers a G(2) checkpoint response, which delays mitotic entry because of insuffici...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.232
更新日期:2010-08-26 00:00:00
abstract::A search for protein tyrosine kinases expressed during murine cardiogenesis resulted in the isolation of a novel tyrosine kinase, designated tek, which maps to mouse chromosome 4 between the brown and pmv-23 loci. The deduced amino acid sequence of tek predicts that it encodes a putative receptor tyrosine kinase that ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::The role of thromboxane A(2) (TxA(2)) in smoking-associated lung cancer is poorly understood. This study was conducted to study the role of TxA(2) in smoking carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-promoted cell survival and growth in human lung cancer cells. We found that NNK increased TxA(2) ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.390
更新日期:2011-01-06 00:00:00
abstract::Low molecular weight protein tyrosine phosphatases (LMW-PTPs) are an enzyme family that plays a key role in cell proliferation control by dephosphorylating/inactivating both tyrosine kinase receptors (such as PDGF, insulin, and ephrin receptors) and docking proteins (such, as beta-catenin) endowed with both adhesion a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207508
更新日期:2004-05-13 00:00:00
abstract::Ataxia-Telangiectasia (A-T) is an autosomal recessive human disease characterized by genetic instability, radiosensitivity, immunodeficiency and cancer predisposition, because of mutation in both alleles of the ATM (ataxia-telangiectasia mutated) gene. The role of Atm heterozygosity in cancer susceptibility is controv...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.280
更新日期:2008-11-20 00:00:00
abstract::Melanoma is the most aggressive form of skin cancer and is notoriously resistant to all current modalities of cancer therapy. A large set of genetic, functional and biochemical studies suggest that melanoma cells become 'bullet proof' against a variety of chemotherapeutic drugs by exploiting their intrinsic resistance...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1206454
更新日期:2003-05-19 00:00:00
abstract::Ki-1 lymphoma is a subtype of human malignant lymphoma characterized by the expression of CD30 (Ki-1) and a peculiar morphology. It is occasionally accompanied by a unique reciprocal chromosomal translocation t(2;5)(p23;q35). A Ki-1 lymphoma cell line, AMS3, was established by maintaining biopsied tumor cells in SCID ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::The translational efficiency of chloramphenicol acetyltransferase (CAT) mRNA containing 5' untranslated region (UTR) sequences of Xenopus c-myc I mRNA was examined in the Xenopus oocyte and early embryo. In contrast to their reduced translation in the oocyte, CAT mRNAs containing 5' UTR sequences located between the c...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLr). Ubiquitin-dependent degradation of the PRLr that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLr on Ser349 followed by the recruitment of the beta-transducin ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209214
更新日期:2006-03-23 00:00:00
abstract::Mdm2, a regulator of the p53 tumor suppressor, is frequently overexpressed in lymphomas, including lymphomas that have inactivated p53. However, the biological consequences of Mdm2 overexpression in lymphocytes are not fully resolved. Here, we report that increased expression of Mdm2 in B cells augmented proliferation...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210788
更新日期:2008-03-06 00:00:00
abstract::An early event of cell migration is characterized as the rapid reorganization of the actin cytoskeleton. Recently, we have demonstrated that rapamycin inhibits tumor cell motility. To understand the underlying mechanism, this study was set to determine whether rapamycin inhibition of cell motility is related to its pr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.137
更新日期:2008-08-28 00:00:00
abstract::Fas-associated death domain (FADD) is an adaptor protein recruiting complexes of caspase 8 to death ligand receptors to induce extrinsic apoptotic cell death in response to a TNF superfamily member. Although, formation of the complex of FADD and caspase 8 upon death stimuli has been studied in detail, posttranslationa...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0323-z
更新日期:2018-09-01 00:00:00
abstract::Imbalances in chromosome 11q occur in approximately 30% of primary neuroblastoma and are associated with poor outcome. It has been suggested that 11q loss constitutes a distinct clinico-genetic neuroblastoma subgroup by affecting expression levels of corresponding genes. This study analysed the relationship of 11q los...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.390
更新日期:2010-02-11 00:00:00
abstract::The Notch ligand delta-like ligand 4 (DLL4) is an essential component expressed by endothelial tip cells during angiogenic sprouting. We have described a conceptually novel therapeutic strategy for targeting tumor angiogenesis and endothelial tip cells based on DNA vaccination against DLL4. Immunization with DLL4-enco...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.176
更新日期:2010-07-29 00:00:00
abstract::Vinexin is an adaptor protein supposed to play pivotal roles in various cellular events such as cell adhesion, cytoskeletal organization, signaling and gene expression. Despite the possible importance, physiological functions and regulatory mechanisms of vinexin are largely unknown. In addition, although vinexin was r...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210512
更新日期:2007-11-01 00:00:00