Abstract:
:Oesopharyngeal brush (OPB) sampling with cytological analysis can yield exfoliated cells from asymptomatic tumours of the upper aero-digestive tract and the oesophagus. In this study, we compared cytological evaluation and molecular analysis for the detection of exfoliated cancer cells sampled with an OPB. A total of 56 patients with a known unique head and neck squamous cell carcinoma (HNSCC) and five healthy controls were enrolled prospectively. Exfoliated cells from these 61 patients were collected with an OPB before initial endoscopy. p53 mutations and UT 5085 microsatellite instability (MI) were analysed in the HNSCC tumour, lymphocytes and the corresponding OPB DNA samples. p53 mutations and UT5085 MI were detected in 31 out of 56 and 14 out of 56 HNSCC, respectively, but not in any of the five controls. Direct sequencing of p53 was able to detect mutations in OPB DNA in only two out of 29 patients harbouring a p53-mutated primary tumour. Microsatellite instability was detected in OPB DNA of 11 out of 13 informative (bandshift detected in tumour) patients, whereas cytological analysis detected abnormal cells in only six of the same 13 patients (P=0.03). In informative patients, all positive OPB samples at cytological analysis were also positive at molecular analysis of UT5085, and both analyses confirmed the two negative samples. Molecular analysis of OPB from eight uninformative patients and from five healthy controls were all negative. OPB sampling with MI-based molecular analysis could be efficient for early detection of recurrent HNSCC. This result prompts us to use other microsatellite markers in order to maximise the percentage of informative patients.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Temam S,Trassard M,Leroux G,Bosq J,Luboinski B,Lenoir G,Bénard J,Janot Fdoi
10.1038/sj.bjc.6600953subject
Has Abstractpub_date
2003-06-02 00:00:00pages
1740-5issue
11eissn
0007-0920issn
1532-1827pii
6600953journal_volume
88pub_type
杂志文章abstract::Obesity has been associated with an increased risk of renal cell cancer among women, while the evidence for men is considered weaker. We conducted a quantitative summary analysis to evaluate the existing evidence that obesity increases the risk of renal cell cancer both among men and women. We identified all studies e...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析
doi:10.1054/bjoc.2001.2040
更新日期:2001-09-28 00:00:00
abstract::We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetect...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600679
更新日期:2003-01-27 00:00:00
abstract::In a study involving 50 breast cancer tumours, we compared two oestrogen receptor (ER) detection methods developed by us--a microplate immunoenzymometric assay (EIA96) and an immunohistochemistry kit (HistoCIS-ER)--with the radioligand assay (RLA), the Abbott immunoenzymometric assay ER-EIA and the reverse transcripta...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.418
更新日期:1997-01-01 00:00:00
abstract::In a case-control study using a large UK primary care database, we found that non-steroidal anti-inflammatory drugs had no protective effect against biliary carcinomas (cholangiocarcinoma and gall bladder cancer). Increased risks were observed for cigarette smoking, diabetes, gallstone disease and obesity. ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604765
更新日期:2009-01-13 00:00:00
abstract:BACKGROUND:The HMGA2 protein has experimentally been linked to EMT and cancer stemness. Recent studies imply that tumour-stroma interactions regulate these features and thereby contribute to tumour aggressiveness. METHODS:We analysed 253 cases of pancreatic ductal adenocarcinoma (PDAC) and 155 cases of ampullary adeno...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2017.140
更新日期:2017-06-27 00:00:00
abstract:BACKGROUND:Faecal immunochemical tests (FITs) have replaced guaiac-based faecal occult blood test (gFOBTs) in several colorectal cancer (CRC) screening programmes. We aimed to evaluate the benefits of this transition based on the Wallonia-Brussels-organised CRC screening programme. METHODS:A total of 1,569,868 individ...
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doi:10.1038/sj.bjc.6604177
更新日期:2008-02-12 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1973.34
更新日期:1973-04-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605855
更新日期:2010-09-07 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6601246
更新日期:2003-09-15 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.280
更新日期:2016-10-11 00:00:00
abstract::Chromosome 9p21 gene copy number in Ewing's sarcoma family of tumour (ESFT) cell lines and primary ESFT has been evaluated using Multiplex Ligation-dependent probe amplification, and the clinical significance of CDKN2A loss and p16/p14(ARF) expression investigated. Homozygous deletion of CDKN2A was identified in 4/9 (...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603819
更新日期:2007-06-18 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600759
更新日期:2003-03-24 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:2012-02-28 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.2012.222
更新日期:2012-06-26 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6603278
更新日期:2006-08-07 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603140
更新日期:2006-05-22 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.208
更新日期:1991-06-01 00:00:00
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pub_type: 杂志文章,多中心研究
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journal_title:British journal of cancer
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更新日期:2011-09-06 00:00:00
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journal_title:British journal of cancer
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更新日期:2011-06-07 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1992-02-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1973.4
更新日期:1973-01-01 00:00:00
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journal_title:British journal of cancer
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更新日期:1998-06-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00