Abstract:
OBJECTIVE:To improve the efficacy of intratympanic therapy using perilymphatic entry through the round window membrane. MATERIAL AND METHODS:The perilymphatic entry characteristics of 2 neutral molecules, mannitol (182.2 Da) and inulin (7,000 Da), were studied. A polyethylene catheter was placed in contact with the guinea pig round window membrane and sealed with cyanoacrylate glue. This catheter was linked to an osmotic minipump that delivered 100 microl portions of 3H-mannitol or 3H-inulin solutions over a 7-day period at a constant rate (0.5 microl/h). Perilymph in the scala vestibuli and scala tympani, cerebrospinal fluid (CSF) and plasma were sampled after 4-7 days of delivery. RESULTS:Despite the constant rate of infusion, perilymphatic radioactivity varied widely from one animal to the other, probably as a function of the position of the microcatheter within the round window niche and/or the permeability of the round window. Even a large molecule such as inulin entered the perilymphatic space. Seven days after the beginning of 3H-mannitol administration, the radioactivity was higher in the perilymph of the scala tympani than in that of the scala vestibuli. The perilymphatic radioactivity on Day 7 was approximately 50% lower than that measured on Day 4 (p = NS). Finally, round window membrane delivery did not preclude distant spread of the molecules into the blood and CSF. CONCLUSIONS:Using round window membrane delivery, the perilymphatic entry of mannitol and inulin depended on their molecular weight. Intratympanic delivery induced a high inter-individual heterogeneity of the drug concentration within the inner ear, with subsequent variability of the therapeutic effects.
journal_name
Acta Otolaryngoljournal_title
Acta oto-laryngologicaauthors
Chelikh L,Teixeira M,Martin C,Sterkers O,Ferrary E,Couloigner Vdoi
10.1080/00016480310001042subject
Has Abstractpub_date
2003-01-01 00:00:00pages
199-202issue
2eissn
0001-6489issn
1651-2251journal_volume
123pub_type
杂志文章abstract::Conclusion Neck dissection after radiotherapy increased the risk of aspiration as a late effect in a sub-sample of patients treated for head and neck cancer in the ARTSCAN study. Patients treated with accelerated fractionation (AF) developed aspiration, with or without coughing, more frequently than patients treated w...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3109/00016489.2015.1113439
更新日期:2016-01-01 00:00:00
abstract::This work describes the different patterns of expression of integrins and extracellular matrix proteins in normal and transformed mucosa in laryngeal and oropharyngeal carcinomas. Samples from each tumor group were sectioned and examined by immunohistochemistry using monoclonal antibodies raised against integrin chain...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480252814298
更新日期:2002-03-01 00:00:00
abstract:OBJECTIVE:To investigate the hearing prognosis of idiopathic sudden sensorineural hearing loss (SSNHL) treated with different initial therapies. METHODS:Subjects consisted of patients diagnosed with idiopathic SSNHL within 7 days from onset and showing severe hearing loss (≥60 dB), who were registered in a Japanese mu...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,多中心研究
doi:10.1080/00016489.2017.1296970
更新日期:2017-01-01 00:00:00
abstract::We investigated the redistribution of facial motor neurons after recovery from a crush injury of the facial nerve in the mongolian gerbil, with the retrograde transportation of horseradish peroxidase (HRP) technique. The facial nerve at the vertical segment was crushed unilaterally for 10 s with forceps. After 1 month...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489509139307
更新日期:1995-03-01 00:00:00
abstract:CONCLUSIONS:The critical stage for cochlear gene regulation in the embryo is E49-E56. These data provide a valuable resource to help elucidate gene regulation in cochlear development and will benefit ear-related studies, especially those that focus on gene and stem cell therapies in hearing loss. OBJECTIVES:To investi...
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pub_type: 杂志文章
doi:10.1080/00016489.2017.1341641
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:The graviceptive otolith function can be measured using subjective visual horizontal (SVH) testing. Nevertheless, more research efforts are required to understand the essential variables affecting SVH. OBJECTIVE:The aim of the present study was to determine the effects of type of visual image and gender on ...
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doi:10.1080/00016489.2020.1817552
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abstract:OBJECTIVE:To investigate the outcome of surgically managed laryngeal carcinoma patients attending an outpatient clinic over a 5-year period. MATERIAL AND METHODS:This was an analytical, retrospective, cross-sectional study. A total of 36 laryngeal carcinoma patients who were managed surgically with or without radiothe...
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pub_type: 杂志文章
doi:10.1080/00016480410018070
更新日期:2004-08-01 00:00:00
abstract::The submeningeal approach through the middle cranial fossa to the internal auditory meatus and cerebello-pontine angle after cutting the tentorium cerebelli is described. It is in some way similar to that used by Morrison & King. The approach is achieved by drilling the petrous bone to the level of the compact plate o...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016487509121344
更新日期:1975-11-01 00:00:00
abstract::As a continuation of our previous work, where we have demonstrated that in chronic otitis media the picture in the submucosa-bone marginal zone is dominated by capillary proliferation and occurrence of a mononuclear, histiocyte-like cell containing lysosome-like cytoplasmatic bodies, we now report the presence of cons...
journal_title:Acta oto-laryngologica
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doi:10.3109/00016487509124704
更新日期:1975-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1080/00016489.2019.1571280
更新日期:2019-03-01 00:00:00
abstract::The aim of the present study is to construct a reference model with the indication for the attitude, the requirements and the resources needed in order to be able to deal with deafness in the presence of disabilities or associated problems. The study group consisted of 13 adults and 18 children affected by profound de...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/03655230410017193
更新日期:2004-04-01 00:00:00
abstract:CONCLUSION:Abnormal caloric and vestibular evoked myogenic potential (VEMP) responses are frequently encountered with a large cerebellopontine angle (CPA) schwannoma, while normal caloric responses and abnormal VEMPs are noted with a large CPA meningioma. This difference may possibly exist because schwannoma causes ves...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480802126553
更新日期:2009-02-01 00:00:00
abstract::In previous studies, sinus secretions have been analysed concerning pO2, pCO2 and pH. In this study the energy metabolism in the maxillary sinus mucosa and secretion was studied in 33 New Zealand White rabbits. In freeze-dried samples, glucose and lactate were analysed by enzymatic assays and ATP by HPLC. In the purul...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488809122271
更新日期:1988-11-01 00:00:00
abstract:CONCLUSIONS:Sensorineural hearing loss must be considered within the clinical picture of systemic lupus erythematosus. The results confirm the usefulness of extended high-frequency audiometry in the audiologic testing of these patients, enabling the possibility of modifying or applying a preventive treatment for a poss...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2016.1219049
更新日期:2017-02-01 00:00:00
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480802552501
更新日期:2009-09-01 00:00:00
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016480902998299
更新日期:2010-01-01 00:00:00
abstract:CONCLUSIONS:In addition to the correlation of transforming growth factor (TGF)-beta1 levels to the radiation toxicity, both Epstein-Barr virus (EBV) infection and postoperative status play roles in the change in plasma TGF-beta1 levels in patients with head and neck cancers. OBJECTIVES:To assess the parameters involve...
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pub_type: 杂志文章
doi:10.1080/00016480701714293
更新日期:2008-10-01 00:00:00
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章,评审
doi:10.3109/00016489.2010.541941
更新日期:2011-06-01 00:00:00
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2016.1154187
更新日期:2016-07-01 00:00:00
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2013.815362
更新日期:2013-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2011.575794
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:The prognostic factors of facial paralysis complicated by middle ear cholesteatoma (MEC) are largely unknown due to limited reports and smaller sample size. OBJECTIVES:This study aimed to analyze prognostic factors of postoperative outcomes of facial paralysis complicated by MEC. MATERIAL AND METHODS:Clini...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2018.1543951
更新日期:2019-02-01 00:00:00
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480600827071
更新日期:2007-03-01 00:00:00
abstract:CONCLUSION:A new murine model for the study of Ménière's disease has been developed by treatment with both lipopolysaccharide (LPS) and aldosterone. Induction of vestibular dysfunction in the hydropic animal model may entail additional stress such as reduced inner ear blood flow, and sudden acute changes in endolymph v...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480701497436
更新日期:2008-03-01 00:00:00
abstract:CONCLUSION:In the present study, about one-third of patients with Meniere's disease developed benign paroxysmal positional vertigo (BPPV)-like attacks. Additionally, more than one-third of all vertigo attacks were BPPV-like attacks. Thus, vertigo attacks in patients with Meniere's disease must be carefully treated beca...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2014.936624
更新日期:2014-11-01 00:00:00
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journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488909127495
更新日期:1989-03-01 00:00:00
abstract::The MRL/lpr mouse, which is homozygous for the recessive lpr genes and has a mutation in the Fas gene encoding a cell-surface receptor for apoptosis, exhibits severe lymphadenopathy and develops systemic lupus erythematosus (SLE)-like disease. It has recently been reported that this mouse also manifests sensorineural ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480310015344
更新日期:2004-01-01 00:00:00
abstract::Background: The management of patients with tinnitus who receive middle ear surgery has not been established.Aims: The aim of this study is (1) how many patients who receive middle ear surgery have tinnitus and (2) how educational counselling and middle ear surgery is effective with consistent tinnitus.Subjects and me...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2020.1713397
更新日期:2020-04-01 00:00:00
abstract::The object of this study was to assess the contributions of various cranial nerve nuclei to the short latency vestibular evoked potentials (VsEPs) in cat. The following nuclei were investigated: vestibular nuclei, and 3rd, 6th and 10th cranial nerve nuclei. In unilateral labyrinthectomized cats, suboccipital craniecto...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489509139277
更新日期:1995-03-01 00:00:00