Abstract：
BACKGROUND:Antisense telomerase RNA (anti-hTR) coupled with 2',5'-linked tetraadenylate (2-5A) shows an inhibitory effect on cell growth and induces apoptosis in certain kinds of cancer. This experiment examined the anti-hTR effect on gastric and colorectal cancer cells. MATERIALS AND METHODS:Gastric cancer cells CRL-5822, CRL-5971 and colorectal cancer cells HTB-38, CCL-247 were treated with repeated doses of anti-hTR. Cell growth and cell cycle parameters were analysed. Telomerase activity was measured by TRAP assay. Control oligonucleotides and normal fibroblast were used as control. RESULTS:After two doses of anti-hTR, the cell viability for CRL-5822, CRL-5971, HTB-38 and CCL-247 cells was reduced to 15%, 12%, 37% and 37%, respectively. The DNA histogram showed cells arrested at the G2/M-phase and the sub-G1 peak found indicated that apoptosis had occurred. CONCLUSION:Anti-hTR demonstrated inhibition of cell growth and cell cycle arrest on gastric and colorectal cancer cells through a telomerase regulation pathway. Its specificity towards cancerous tissues but not normal tissues suggested a potential for anti-cancer therapy.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Wong SC,Yu H,Moochhala SM,So JBsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
465-9issue
1Aeissn
0250-7005issn
1791-7530journal_volume
23pub_type
杂志文章abstract：BACKGROUND:The concepts of vasculogenic mimicry and mosaic vessels have been proposed as novel modes of tumour neovascularisation. However, the presence and significance of these types of neovascularisation remain unclear. MATERIALS AND METHODS:ECV304 human bladder carcinoma cells were used to determine how tumour cel...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:P44 Ro (Mel) is a human malignant melanoma cell line derived from a testicular metastasis in a DNA repair deficient, xeroderma pigmentosum patient. This line harbors a N-ras gene mutated in codon 61. To investigate other cellular genes possibly contributing to the expression of its transformed phenotype, four XP44 rev...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：BACKGROUND/AIM:Advanced anaplastic thyroid cancer (ATC) is a rare, but highly aggressive malignancy, and its prognostic factors need to be further explored. We examined socioeconomic factors' predictive effect for survival performance in stage IV ATC patients. MATERIALS AND METHODS:Using the Surveillance, Epidemiology...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13198
更新日期：2019-02-01 00:00:00
abstract：BACKGROUND/AIM:Although the advent of Helicobacter pylori eradication and global societal changes are widely assumed to impact on gastric cancer (GC)-related mortality, there is remarkable little quantitative and qualitative insight into the nature of its effects. Here, we exploited a nationwide reporting system to inv...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11871
更新日期：2017-08-01 00:00:00
abstract：:Cytotoxic efficacy of anticancer drugs has been widely studied with monolayer-cultured cancer cells. However, the drug efficacy under two-dimensional (2D) culture conditions usually differs from that of three-dimensional (3D) culture conditions. In the present study, an in vitro tumor tissue model was constructed by s...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND/AIM:Previous studies have revealed that zinc finger and BTB domain-containing 7A (ZBTB7A), an important proto-oncogene, plays multiple roles in carcinogenesis and is up-regulated in several human malignancies. However, the expression of ZBTB7A in colorectal carcinoma (CRC) has seldom been documented. In this...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12522
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Genomic imprinting is associated with many human diseases, including various types of cancers, however, no studies on gene imprinting are related to squamous cell carcinoma of the head and neck (SCCHN) directly. MATERIALS AND METHODS:In this study, the expression of a panel of 15 imprinted genes in cancerou...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：AIM:Mitotane is used in adrenal cancer as adjuvant therapy, monotherapy or combined with other cytotoxic agents in advanced disease, but only 30% of patients respond. The aim of this study was to define the structural requirements for drug activity and to develop analogs with improved adrenalytic action. MATERIALS AND...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND:A drawback of cancer gene therapy is the failure of toxic gene introduction into a proportion of the tumor cells, resulting in re-progression of the disease. Cancer cell-specificity of the gene introduction has also been problematic. MATERIALS AND METHODS:Previously defined promoter/enhancer DNA of Q5 tumor...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-04-01 00:00:00
abstract：:We have reported the presence of a CD4+ subpopulation of Natural Killer cells (NK) in humans. These cells also express HIV coreceptors CCR5 or CXCR4 and are infectable by HIV-1. Infection is productive and persistent in vitro and in vivo. Infected people under therapy have evidence of viral DNA in their NK pool. In vi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:Male wap-ras transgenic mice develop adenocarcinomas in salivary and/or mammary tissue by age 1 year. When the wap-ras transgene was bred into the FVB/N strain, males developed multiple mammary tumors between 1.5 and 3 mo. of age, but no salivary tumors. Crosses between ras/FVB mice and other strains produced moderate...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:The purpose of this study was to elucidate the behavior of signal transduction activity in rat and human carcinoma cells. Signal transduction activity was measured by the steady-state activity of the three enzymes involved in the conversion of 1-phosphatidylinositol (PI) to IP3, PI 4-kinase, PI 4-phosphate 5-kinase, a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:The technical performance and clinical usefulness of the newly developed Elecsys CA 125 II assay (Boehringer Mannheim) was evaluated in a multicenter study. Imprecision studies were carried out using control sera and human pool sera with CA 125 concentrations from 11 to 1026 U/ml. Within-run CVs between 0.7 to 4.8% (m...
journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：1999-07-01 00:00:00
abstract：:We have developed a sensitive enzyme-linked immunosorbent assay (ELISA) procedure that uses a commercially-available anti-BrdUrd antibody for the quantitation of BrdUrd substituted into DNA. In our assay, 50% displacement occurs at 0.89 nM of BrdUrd in 2.2% BrdUrd-substituted DNA, which is equivalent to 2.47 ng of Brd...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：BACKGROUND:Dendritic cell (DC) mono-immunotherapy has not been successful so far in ovarian cancer. The addition of a toll-like receptor (TLR) agonist has the potential to boost the innate immune system, in addition to the adoptive immune response initiated by DCs. MATERIALS AND METHODS:ID8-fLuc C57BL/6 mice were inje...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11162
更新日期：2016-11-01 00:00:00
abstract：:Metastatic spread of malignancy is the primary cause of treatment failure and subsequent death in cancer patients. All cancers have the capability to metastasize, however, there are notable exceptions which rarely if ever metastasize. These include basal cell carcinoma, or cancers which are primarily locally invasive ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND/AIMS:Our study aimed to compared 3-year results following laparoscopy-assisted proximal gastrectomy (LAPG)-double tract anastomosis (DTA) and lower esophageal sphincter-preserving LAPG (LES-LAPG). PATIENTS AND METHODS:Nine patients who underwent LES-LAPG and 23 LAPG-DTA were compared regarding 3-year follow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13129
更新日期：2019-01-01 00:00:00
abstract：BACKGROUND:Incidence of brain metastases (BM) from breast cancer (BC) is increasing. However, prognostic evaluation and treatment strategies are still a matter of debate. AIM:To describe the clinical outcome of BM from BC treated by neurosurgical resection and to identify the actual prognostic factors in this specific...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
abstract：BACKGROUND:Live animal studies using an inoculation model of breast cancer indicate that anaesthetic drugs and techniques differentially affect cancer metastasis, inversely related to Natural Killer (NK) cell and T lymphocyte levels. Clinical histological studies demonstrate that the distribution of these immune cells ...
journal_title：Anticancer research
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2015-03-01 00:00:00
abstract：:The value of metabolic proton NMR spectroscopic imaging to detect and classify tumours increases with the spatial resolution and the information content of the spectra. Several factors influencing these quantities are discussed. ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1996-05-01 00:00:00
abstract：AIM:We aimed to investigate whether 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT) positron emission tomography (PET) can estimate thymidine kinase 1 (TK1) activity after thymidylate synthase (TS) inhibition by 5-fluorouracil (5-FU) in a mouse tumor model. MATERIALS AND METHODS:Nude mice with HT29 tumors were injecte...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
abstract：:We investigated the immunohistochemical expression of p21/waf1 protein in 59 cases of nasopharyngeal carcinomas (NPC) and compared p21 expression with PCNA, p53 and mdm2 protein expression. We found p21, PCNA, p53 and mdm2 in 59/59, 59/59, 18/59 and 12/59 nasopharyngeal carcinomas, respectively. We observed a tendency...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：BACKGROUND:Human carcinomas not only consist of neoplastic epithelial cells but also of tumor stroma, which may play an important role in tumor-progression. Whereas the tumor surrounding stroma is generally believed to represent a reactive component induced by tumor cell derived factors, a contribution of neoplastic ce...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：BACKGROUND:Estramustine is an anti-mitotic cytostatic drug that also enhances the effect of radiotherapy. The mechanism of radiosensitization is not thoroughly known. Since both radiotherapy and estramustine induce apoptosis in prostate cancer cells, we conducted an experiment to show whether radiosensitization is medi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：BACKGROUND/AIM:To identify predictors of local control and survival after whole-brain irradiation (WBI) for cerebral metastases from cancer of unknown primary (CUP). PATIENTS AND METHODS:In 140 patients receiving WBI alone or following resection, seven factors were investigated including treatment approach, WBI-regime...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12492
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND:Large adrenal tumors (LATs, ≥6 cm) are uncommon and associated with malignancy in 25% of cases. Their surgical management remains debatable. The aim of the present report was to evaluate the current incidence, nature and management of LAT. PATIENTS AND METHODS:We carried out a retrospective review of LATs m...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2014-05-01 00:00:00
abstract：:Bisphosphonates are strong inhibitors of osteoclastic bone resorption in both benign and malignant bone diseases. The nitrogen-containing bisphosphonates (N-BPs) have strong cytotoxicity via inhibition of protein prenylation in the mevalonate pathway, and also demonstrate direct cytostatic and proapoptotic effects on ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-04-01 00:00:00
abstract：BACKGROUND:Activating Gs a mutations have been identified in most instances of fibrous dysplasia (FD). This mutation leads to consistently elevated intracellular cyclic adenosine monophosphate (cAMP) levels, with various biological consequences. The development of secondary sarcoma in FD is a rare but well-established ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-03-01 00:00:00
abstract：:Glucose consumption and tritiated thymidine incorporation were monitored for 54 days in histocultured human superficial transitional cell carcinoma of the bladder, indicating that long-term histoculture of human transitional cell carcinoma of the bladder is feasible. The glucose-consumption rate was relatively similar...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Our recent in vitro findings for suppression of thrombospondin-1 (TSP1; an antiangiogenic factor) expression by wild-type (wt) p53 in a p53-null thyroid carcinoma cell line, FRO, prompted us to investigate the in vivo effect of exogenous wt-p53 and TSP1 expression on tumor growth and angiogenesis of FRO xenografts in ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
