Abstract：
:We have developed a sensitive enzyme-linked immunosorbent assay (ELISA) procedure that uses a commercially-available anti-BrdUrd antibody for the quantitation of BrdUrd substituted into DNA. In our assay, 50% displacement occurs at 0.89 nM of BrdUrd in 2.2% BrdUrd-substituted DNA, which is equivalent to 2.47 ng of BrdUrd-containing DNA. A fluorescein isothiocyanate-conjugated anti-BrdUrd antibody was used to determine the labeling index of cultured cells and in vivo tumors treated with BrdUrd. Combining results of the ELISA procedure (to determine the percent BrdUrd substitution), and flow cytometry (to determine the percentage of cells that incorporated BrdUrd) we found that BrdUrd incorporated into the DNA of cells in vitro and tumors in vivo could be quantitated with precision.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Cheitlin RA,Bodell WJsubject
Has Abstractpub_date
1988-05-01 00:00:00pages
471-4issue
3eissn
0250-7005issn
1791-7530journal_volume
8pub_type
杂志文章abstract：:The radical modulation activity of hot water and NaOH extracts of the thorn apple of Crataegus Cuneata Sieb. et. Zucc. was investigated, using ESR spectroscopy. The radical intensity of sodium ascorbate was slightly reduced at lower concentrations of the extracts, but it was synergistically enhanced at higher concentr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：AIM:To investigate frequent quantitative alterations of intestinal-type gastric adenocarcinoma. MATERIALS AND METHODS:We analyzed genome-wide DNA copy numbers of 22 samples and using CytoScan® HD Array. RESULTS:We identified 22 gene alterations that to the best of our knowledge have not been described for gastric can...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：BACKGROUND/AIM:The utility of nanoparticle albumin-bound paclitaxel (nab-PTX) monotherapy in patients with relapsed small-cell lung cancer (SCLC) has not been fully evaluated. We aimed to investigate the efficacy and safety of nab-PTX monotherapy in relapsed SCLC patients, including heavily treated patients. PATIENTS ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.14105
更新日期：2020-03-01 00:00:00
abstract：BACKGROUND:The size of colorectal polyps is important in the clinical management of these lesions. When using a conventional ruler (the tool of pathologists worldwide), we have previously found unacceptably high intra- and inter-observer variations in assessing the size of phantom polyps. The aim of this study was to a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：:The di-sodium phosphate pro-drug of combretastatin-A4(combA-4P) is undergoing Phase 1 clinical trial in the USA and UK. Its mechanism of action is thought to be related to tubulin-binding properties that result in rapid, tumour endothelial cell damage, neovascular shutdown and subsequent haemorrhagic necrosis. Drugs t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:Breast cancer is the most frequent cancer and the second leading cause of cancer deaths in women today. A number of 1,4-naphthoquinone derivatives have been found to possess significant pharmacological effects associated with marked antimicrobial and antitumor activities. In the present study, the in vitro e...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND/AIM:Trans-hiatal and -cervical approach mediastinoscopic radical esophagectomy (TMrE) for esophageal cancers is a less-invasive procedure and does not require for trans-thoracic approach management. However, some patients suffer from pleural effusion after TMrE. In the present study, we investigated the clin...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13069
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:Interleukin-2 (IL-2) and interferon-alpha (IFN-alpha) are the main immuno-biological agents used in the therapy of metastatic renal cell carcinoma (RCC). Unfortunately the promising results obtained in biological studies have not yet been confirmed in clinical studies. One reason is linked to the immunosuppr...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2002-09-01 00:00:00
abstract：:Inv(3)(q21q26)/t(3;3)(q21;q26) is a feature of a distinctive entity of acute myeloid leukemia (AML) associated with normal or elevated platelet count, atypical megakaryocytes and multilineage dysplasia in the bone marrow, as well as minimal to no response to chemotherapy and poor clinical outcome. The presence of an i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-02-01 00:00:00
abstract：:VP-16 and ICRF-193 are different types of antitumor topoisomerase II inhibitors, being cleavable and non-cleavable complex-stabilizing types, respectively. To examine the possibility of enhancing the efficacy of combination chemotherapy, we carried out simultaneous and sequencial treatment of cells with the two drugs....
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:Cisplatin is the most active anticancer agent for lung cancer. It has been reported that intracellular accumulation of cisplatin is important in determining resistance to cisplatin, which may be modulated by Na+, K(+)-ATPase activity. On the other hand, it is well-known that sorbitol, a metabolite of glucose mediated ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:This review focuses on the functional role and structural features of the genes involved in common hereditary cancers. Most of these tumors are sporadic and the genetic alterations responsible for their genesis take place over several cell generations; nevertheless, 5 to 10% of the human tumors are hereditary, with a ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2000-11-01 00:00:00
abstract：:The antitumor effects of the somatostatin structural derivative TT-232 in different rodent and xenograft tumor models are summarized in this report. TT-232 had previously been shown to inhibit the proliferation of a large number of cancer cell lines in vitro and reduce the size of different tumors in animal models in ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：BACKGROUND:In patients with melanoma, early dissemination via lymphatic and hematogenous routes is frequently seen. Thus, besides clinical follow-up examination and imaging, reliable melanoma-specific serological tumor markers are needed. PATIENTS AND METHODS:We retrospectively compared two serum markers for melanoma,...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11918
更新日期：2017-09-01 00:00:00
abstract：AIM:The evaluation of toxicity after high-dose-rate interstitial brachytherapy (HDR-ISBT) as monotherapy for localized prostate cancer. MATERIALS AND METHODS:We analyzed early and late toxicities in 100 patients treated by HDR-ISBT as monotherapy at the National Hospital Organization Osaka National Hospital using both...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：BACKGROUND/AIM:Numerous trials have described a wide variation of metabolic complications associated with the mammalian target of rapamycin inhibitors (mTORi). This analysis aimed to report and critically analyze the risks of mTORi-associated metabolic complications. MATERIALS AND METHODS:A comprehensive search of all...
journal_title：Anticancer research
pub_type： 杂志文章,meta分析
doi：
更新日期：2016-04-01 00:00:00
abstract：:A series of simple alpha, beta-unsaturated carbonyl compounds (1-26) was characterized for their cytotoxic profiles against oral human normal and tumor cells. Several cycloalkenones showed potent cytotoxic activities against human oral squamous cell carcinoma HSC-2 cell line. Among them, 4,4-dimethyl-2-cyclopenten-1-o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:The role of serum tumor markers in lung cancer has not been defined. It is widely believed that almost all neoplasms produce at least a few substances, but the identity of most of the known substances has not been correlated with a positive role in either the diagnosis or prognosis of patients with these neoplasms. We...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：BACKGROUND:Despite many years of clinical research and development, nausea and vomiting remain challenging toxicities related to chemotherapy. The aim of our study was to clarify the significance of non-pharmacological, patient-related risk factors for chemotherapy-induced nausea and vomiting. Furthermore, we aimed to ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12798
更新日期：2018-08-01 00:00:00
abstract：BACKGROUND:Genomic imprinting is associated with many human diseases, including various types of cancers, however, no studies on gene imprinting are related to squamous cell carcinoma of the head and neck (SCCHN) directly. MATERIALS AND METHODS:In this study, the expression of a panel of 15 imprinted genes in cancerou...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：:The reverse transcriptase polymerase chain reaction (RT-PCR) can be of clinical relevance in identifying malignant melanoma cells in blood or tissues of patients at risk for disseminated melanoma. The diagnostic value of this marker however, is still controversial. The objective of this study was to compare and quanti...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：BACKGROUND/AIM:The differentiation of the mouse breast epithelial cell line HC11 is known to require confluence as well as the addition of hydrocortisone, insulin and prolactin. MATERIALS AND METHODS:Since confluence, which triggers the engagement of the cell-to-cell adhesion molecule E-cadherin, induces a dramatic in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13401
更新日期：2019-06-01 00:00:00
abstract：:A total of 2090 patients with endometrial carcinoma were followed-up for at least five years. The treatment modalities, as well as the results of treatment, regarding 272 patients with disease relapse are presented. The results are not encouraging. We found no statistically significant difference regarding overall sur...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：:MOv18, a non-internalizing monoclonal antibody (MAb) with restricted tumor specificity, was conjugated to ricin toxin (RT). According to their ability to bind to galactose residues of Sepharose 6B, the immunoconjugates were fractionated into Bound and Unbound MOv18-RT. The two conjugates could be distinguished by SDS-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Para-aminobenzoic acid (PABA) has been previously reported as being an inhibitor of DDP toxicity, and its use did not result in any observable loss in antitumor activity of DDP. The following studies investigated the effect of PABA on the pharmacokinetics and urinary excretion of cis-diamminedichloroplatinum(II) (DDP)...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：BACKGROUND/AIM:Osteosarcoma originates from mesenchymal stem cells with impaired bone differentiation. In the present study we investigated the effect of ascorbic acid (AsA) on osteogenic differentiation and apoptosis of the MG-63 osteosarcoma cell line. MATERIALS AND METHODS:We evaluated the expression of runt-relate...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：:ADCP is a dimeric glycoprotein of about 200KD, for which the physiological role is still obscure. This protein occurs mainly in a membrane bound form in various human tissues. In this paper we review the current literature on ADCP in cancer studies. Soluble ADCP was described to be consistently decreased or absent in ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1986-09-01 00:00:00
abstract：BACKGROUND:As a majority of ovarian cancer patients will ultimately develop recurrent disease, there is an urgent need for alternative or additional approaches in the treatment of this cancer. MATERIALS AND METHODS:The antitumour effect of i.p. administered cisplatin, liposomal muramyltripeptide phosphatidylethanulami...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:In a previous study we demonstrated spontaneous fragility and hypersensitivity to fragile site induction by aphidicolin in lymphocytes from some neuroblastoma patients and their parents. Here we report data based on a total of 40 patients and 37 families. Possible correlations between higher sensitivity to aphidicolin...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：BACKGROUND:To understand the molecular response of tumor cells to therapeutic ionizing radiation (IR), we previously reported that human breast cancer cells derived following chronic exposure to fractionated ionizing radiation (MCF+FIR) showed a transient radioresistance. MCF+FIR cells also demonstrated increased activ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
