Evaluation of a dual hemoglobin A(2)/A(1c) quantitation kit on the bio-rad variant II automated hemoglobin analyzer.

Abstract:

CONTEXT:Quantitation of hemoglobin (Hb) A(1c) and investigation of hemoglobinopathy on the Bio-Rad Variant analyzers require a switch between 2 separate kits that is time consuming and causes errors. OBJECTIVE:Evaluation of a new Variant II HbA(2)/HbA(1c) Dual kit capable of both Hb A(1c) quantitation and hemoglobinopathy investigation on a single kit. DESIGN:We evaluated Hb A(1c), Hb A(2), and Hb F quantitation for precision, linearity, and correlation with current methodology. We also evaluated detection of Hb variants and correlation of Hb Barts quantitation. SETTING:Hamilton Regional Laboratory Medicine Program, Provincial Hemoglobinopathy Laboratory, St Joseph's Healthcare Site, Hamilton, Ontario. PATIENTS:Patient blood samples submitted for Hb A(1c) quantitation or hemoglobinopathy investigation. MAIN OUTCOME MEASURES:Precision, linearity, linear regression, and reference interval validation. RESULTS:We provide tables and figures illustrating precision, linearity, linear regression, and quantitation of Hb variants. We validated reference intervals for Hb A(1c), Hb A(2), and Hb F. CONCLUSIONS:The dual kit provides precise Hb A(1c), Hb A(2), and Hb F quantitation. The results show good linearity and correlate well with the results of current methods. We detected all clinically important Hb variants and a wide variety of rare variants. The dual kit has several advantages: it eliminates the need for extensive kit switch over; improves utility for newborn screening because of its quantification of Hb Barts; permits quantification of Hb A(1c) using the beta-Thal method; and eliminates the need for separate Hb A(2) reference intervals for patients with Hb S because of its accurate quantitation of Hb A(2) in the presence of Hb S.

journal_name

Arch Pathol Lab Med

authors

Lafferty JD,McFarlane AG,K Chui DH

doi

10.1043/0003-9985(2002)126<1494:EOADHA>2.0.CO;2

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

1494-500

issue

12

eissn

0003-9985

issn

1543-2165

journal_volume

126

pub_type

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