Abstract:
CONTEXT.—:Resection of breast carcinoma with adequate margins reduces the risk of local recurrence and reoperation. Tozuleristide (BLZ-100) is an investigational peptide-fluorophore agent that may aid in intraoperative tumor detection and margin assessment. In this study, fluorescence imaging was conducted ex vivo on gross breast pathology specimens. OBJECTIVES.—:To determine the potential of tozuleristide to detect breast carcinoma in fresh pathology specimens and the feasibility of fluorescence-guided intraoperative pathology assessment of surgical margins. DESIGN.—:Twenty-three patients received an intravenous bolus dose of 6 or 12 mg of tozuleristide at least 1 hour before surgery. Fifteen lumpectomy and 12 mastectomy specimens were evaluated for fluorescence by the site's clinical pathology staff using the SIRIS, an investigational near-infrared imaging device. The breast tissue was then processed per usual procedures. Fluorescent patterns were correlated with the corresponding hematoxylin-eosin-stained sections. Clinical pathology reports were used to correlate fluorescent signal to grade, histotype, prognostic marker status, and margin measurements. RESULTS.—:Tozuleristide fluorescence was readily observed in invasive and in situ breast carcinoma specimens. Most invasive carcinomas were bright and focal, whereas in situ lesions demonstrated a less intense, more diffuse pattern. Tozuleristide was detected in ductal and lobular carcinomas with a similar fluorescent pattern. Fluorescence was detected in high- and low-grade lesions, and molecular marker/hormone receptor status did not affect signal. Fluorescence could be used to identify the relationship of carcinoma to margins intraoperatively. CONCLUSIONS.—:Tumor targeting with tozuleristide allowed visual real-time distinction between pathologically confirmed breast carcinoma and normal tissue.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Dintzis SM,Hansen S,Harrington KM,Tan LC,Miller DM,Ishak L,Parrish-Novak J,Kittle D,Perry J,Gombotz C,Fortney T,Porenta S,Hales L,Calhoun KE,Anderson BO,Javid SH,Byrd DRdoi
10.5858/arpa.2018-0197-OAsubject
Has Abstractpub_date
2019-09-01 00:00:00pages
1076-1083issue
9eissn
0003-9985issn
1543-2165journal_volume
143pub_type
杂志文章abstract:CONTEXT:Perineural invasion and vascular invasion may be adverse prognostic factors in patients with oral cavity squamous cell carcinoma. However, the incidence of perineural and vascular invasion varies in the literature, and the use of immunohistochemistry to enhance their detection has not been evaluated in oral cav...
journal_title:Archives of pathology & laboratory medicine
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doi:10.1043/1543-2165(2005)129<354:PAVIIO>2.0.CO;2
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abstract:CONTEXT:- At our medical center, cytopathologists perform rapid on-site evaluation for specimen adequacy of fine-needle aspiration and touch imprint of needle core biopsy lung cancer samples. Two years ago the molecular diagnostics laboratory at our institution changed to next-generation sequencing using the Ion Torren...
journal_title:Archives of pathology & laboratory medicine
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doi:10.5858/arpa.2016-0096-OA
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abstract:OBJECTIVE:To compare the ability of the Vitek GPS-TB card with disk diffusion testing for determining the susceptibility of enterococci to vancomycin. DESIGN:Vitek susceptibility testing was performed using the GPS-TB card and software version R05.03. Disk diffusion susceptibility testing was performed according to Na...
journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
doi:10.1043/0003-9985(1999)123<0622:COTVGT>2.0.CO;2
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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abstract::Public fascination with and support for genetic medicine is complicated by a deeply held fear that genetic information will be used by third parties (eg, insurers, employers, school systems) in ways that will harm the individuals from whom it was derived. Since the mid-1990s there has been much state and some federal ...
journal_title:Archives of pathology & laboratory medicine
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doi:10.1043/0003-9985(1999)123<1066:ETRTCA>2.0.CO;2
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abstract::We report a case of "uncombable hair syndrome" that occurred in a boy who had no other abnormalities. The diagnosis was clinically suspected and confirmed by examination of the scalp hair, which was treated with a bath of gold. Scanning electron microscopy was used for examination. The main defect of the hair was a lo...
journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1987-08-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1977-09-01 00:00:00
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更新日期:1976-03-01 00:00:00
abstract::Thoracic manifestations of rheumatic disease (RD) are increasingly recognized as a significant cause of morbidity and mortality worldwide. Rheumatologic underpinnings have been identified in a significant proportion of patients with interstitial lung disease. The 5 RDs most frequently associated with pleuropulmonary d...
journal_title:Archives of pathology & laboratory medicine
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doi:10.5858/arpa.2012-0248-SA
更新日期:2012-10-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract::The postmortem tissue distribution of cholesterol precursors, squalene, and methyl sterols was quantitated in ten human subjects by the use of gas-liquid chromatography. Five methyl sterol subfractions were found, with each organ having a pattern of its own. The mean esterification percentage of the methyl sterol mixt...
journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1980-01-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1988-04-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::We describe an infarcted mass in the testis containing "ghost" cells suspicious for neoplasm. The entire lesion was necrotic. A Masson trichrome stain greatly improved nuclear and cytologic detail, confirming the suspicion of neoplasm. Placental alkaline phosphatase revealed specific membrane staining of the neoplasti...
journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
doi:10.1043/0003-9985(2002)126<0205:NSOTT>2.0.CO;2
更新日期:2002-02-01 00:00:00
abstract::It is well known that neoplastic cells of several organs produce amylases. However, to our knowledge, alpha-amylase expression has not been reported in hepatic neoplasms. In the present study, we surveyed the expression of alpha-amylase isoenzymes in cholangiocarcinoma (CCC) (n = 18) and hepatocellular carcinoma (n = ...
journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1993-02-01 00:00:00
abstract:CONTEXT:Current regulatory proficiency testing scoring results in an automatic failure for identifying high-grade squamous intraepithelial lesion (HSIL) as negative. OBJECTIVE:The College of American Pathologists Interlaboratory Comparison Program in Cervicovaginal Cytology data from January 2004 to April 2005 were an...
journal_title:Archives of pathology & laboratory medicine
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doi:10.1043/1543-2165(2006)130[1114:TPFFIG]2.0.CO;2
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journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1995-03-01 00:00:00
abstract:CONTEXT:-Low interobserver diagnostic agreement exists among high-grade endometrial carcinomas. OBJECTIVE:-To evaluate diagnostic variability in International Federation of Gynecology and Obstetrics (FIGO) grade 3 endometrioid adenocarcinoma (G3EC) in 2 different sign-out practices. DESIGN:-Sixty-six G3EC cases were ...
journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1998-01-01 00:00:00
abstract::A 54-year-old woman presented with a nasal mass. Biopsy demonstrated undifferentiated tumor cells with extensive apoptosis and necrosis. Chromosome analysis identified a 46,XX,t(15;19)(q13;p13.1) pattern. Nuclear protein in testis (NUT) immunohistochemistry and fluorescence in situ hybridization confirmed NUT rearrang...
journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1997-07-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
doi:
更新日期:1986-11-01 00:00:00
abstract:CONTEXT:Impairment of venous outflow manifests as zone 3 sinusoidal dilatation and congestion (SDC) in liver biopsy. However, the finding of SDC is not specific for venous outflow impairment.Objectives.-To determine the specificity of SDC in liver biopsies for venous outflow impairment and to seek an explanation for SD...
journal_title:Archives of pathology & laboratory medicine
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更新日期:2004-08-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章,评审
doi:10.5858/arpa.2013-0359-SA
更新日期:2014-04-01 00:00:00
abstract::Most mesenchymal tumors of the gastrointestinal tract are now referred to as gastrointestinal stromal tumors (GISTs). The tumors differ from ordinary leiomyomas and schwannomas in several respects: the GISTs typically express c-kit protein (CD117) and CD34, 30% to 50% of them are (often focally) positive for alpha-smo...
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更新日期:2000-06-01 00:00:00