Abstract:
:Variants of growth hormone (GH) are present in most vertebrates. Chicken GH (cGH) undergoes posttranslational modifications that contribute to its structural diversity. Although the 22-kDa form of GH is the most abundant, some other variants have discrete bioactivities that may not be shared by others. The proportion of cGH variants changes during ontogeny, suggesting that they are regulated differentially. The effect of growth hormone-releasing hormone (GHRH) on the release of cGH variants was studied in both pituitary gland and primary cell cultures, employing sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blotting, and densitometry. GHRH (2 nM, 2 h) stimulated the secretion of most of the size variants of cGH although the amplitude of increase was not equal for each one. A differential effect on the secretion of GH size variants, particularly on the 22- (monomer) and 26-kDa (putatively glycosylated) cGH isoforms was found in both systems. In the whole pituitary culture, the proportion of the 26-kDa immunoreactive cGH increased 35% while the 22 kDa decreased 31% after GHRH treatment in comparison with the controls. In the primary cell culture system, the proportion of the glycosylated variant increased 43% whereas the monomer and the dimer decreased 22.26 and 29%, respectively, after GHRH stimulation. Activators of intracellular signals such as 1 mM 8-bromo-cAMP and 1 microM phorbol myristate acetate had a similar effect to that obtained with GHRH. The data support the hypothesis that GH variants may be under differential control and that GHRH promotes the release of a glycosylated cGH variant that has an extended half-life in circulation.
journal_name
Endocrinejournal_title
Endocrineauthors
Martínez-Coria H,López-Rosales LJ,Carranza M,Berumen L,Luna M,Arámburo Cdoi
10.1385/ENDO:17:2:91subject
Has Abstractpub_date
2002-03-01 00:00:00pages
91-102issue
2eissn
1355-008Xissn
1559-0100pii
ENDO:17:2:91journal_volume
17pub_type
杂志文章相关文献
ENDOCRINE文献大全abstract:CONTEXT:To prepare for radioactive iodine therapy in post total thyroidectomy patients with well-differentiated thyroid cancer (WDTC), either thyroid hormone withdrawal (THW) or administration of recombinant human thyrotropin (rhTSH) can be performed. OBJECTIVE:Our objective is to compare quality of life (QoL) paramet...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-020-02238-z
更新日期:2020-07-01 00:00:00
abstract::Initiation and maintenance of labor in humans is associated with an increase in prostaglandin synthesis by intrauterine tissues. The objective of the present study was to characterize the distribution of membrane-bound PGES (mPGES) protein and mPGES mRNA in human placenta, fetal membranes, and decidua at term and to d...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:20:3:219
更新日期:2003-04-01 00:00:00
abstract:INTRODUCTION:We retrospectively evaluated all patients with pituitary tumours treated in our department from 1/1/1997 to 01/11/2014. PATIENTS AND METHODS:Two hundred and fifteen patients (124 females, 91 males, mean age 50.9 years) were treated because of pituitary tumours. All patients underwent basal hormonal analys...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-018-1570-z
更新日期:2018-05-01 00:00:00
abstract::Obstructive sleep apnea syndrome (OSAS) is a common health problem, and associated with obesity, metabolic syndrome (MetS), and diabetes. Growing evidence shows that 25-hydroxyvitamin-D3 (25-OH-D) insufficiency and high parathyroid hormone (PTH) levels may be correlated to glucose intolerance, MetS, obesity, and cardi...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-013-0022-z
更新日期:2014-03-01 00:00:00
abstract::The aim of this article is to investigate the effects of Aminoguanidine and vitamin C (VitC) on type IV collagen in diabetic nephropathy rats. Diabetic nephropathy rats were induced by intraperitoneal injection of STZ. Rats were randomly divided into five groups: normal control group (n = 10), diabetes group (n = 10),...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-010-9419-0
更新日期:2011-06-01 00:00:00
abstract::Thickened pituitary stalks (TPSs) on magnetic resonance imaging (MRI) result from diverse pathologies; therefore, it is essential to make specific diagnoses for clinical decision-making. The diagnoses and indications for surgical biopsies in patients with central diabetes insipidus (CDI) and TPSs are thoroughly discus...
journal_title:Endocrine
pub_type: 信件
doi:10.1007/s12020-014-0184-3
更新日期:2014-09-01 00:00:00
abstract::Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) are synthesized and released from adrenal cells. Therefore, the effects of TNF-alpha and IL-6 on cortisol release from bovine zona fasciculata (ZF) cells were investigated. IL-6 (10-1000 pg/mL) significantly increased basal and adrenocorticotropic hormon...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:13:3:369
更新日期:2000-12-01 00:00:00
abstract::It is well established that hypothalamic dopamine (DA) is the major physiologic regulator of prolactin (PRL) secretion, exerting a tonic inhibition throughout most of the estrous cycle. A dramatic drop in the amount of DA perfusing the anterior pituitary occurs in the afternoon of proestrus and is critical for the pro...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:20:1-2:67
更新日期:2003-02-01 00:00:00
abstract::RF amide peptide family with distinctive terminal -Arg-Phe-NH(2) signature is evolutionarily conserved from invertebrates to mammals. These neuropeptides have been shown to affect diverse functions in invertebrates and vertebrates including influencing pituitary hormone secretion. More recently, two members of this fa...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-012-9672-5
更新日期:2012-12-01 00:00:00
abstract::Cytoplasmic calcium ([Ca(2+)](i)) changes evoked by adenosine 5(1)-triphosphate (ATP) were recorded in cultured individual Leydig cells within 10-18 h after cell dispersion. [Ca(2+)](i) was monitored using Fura-2AM loaded cells with a digital ratio imaging system. Five micromolars ATP induced biphasic [Ca(2+)](i) resp...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738690
更新日期:1996-06-01 00:00:00
abstract::Prolactinomas are the most common secretory pituitary tumors; however, their pathogenesis is unclear. In order to explore the pathogenesis of prolactinomas, we used fiber-optic BeadArray to examine gene expression profiles in five prolactinomas compared with three normal pituitaries. Three down-regulated genes and one...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-010-9389-2
更新日期:2010-12-01 00:00:00
abstract::Chronic stress can have a deleterious effect on the re-productive axis that, for females, is manifested in an increased incidence of infertility. However, gonadal steroids may, in turn, affect a female's response to stress as measured by activity within the limbic-hypothalamic-pituitary-adrenal (LHPA) axis. What is no...
journal_title:Endocrine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1385/ENDO:26:2:089
更新日期:2005-03-01 00:00:00
abstract::A substantial number of pregnancies are complicated by gestational diabetes mellitus (GDM) and up to 70 % of women with GDM go on to develop type 2 diabetes. Given the extensive body of research suggesting physical activity reduces the risk of type 2 diabetes, facilitating physical activity, and reducing sedentary tim...
journal_title:Endocrine
pub_type: 杂志文章,评审
doi:10.1007/s12020-015-0828-y
更新日期:2016-04-01 00:00:00
abstract::Hyponatremia has been observed in elderly patients treated with the selective serotonin reuptake inhibitor (SSRI) fluoxetine. The pathogenesis of this effect is not known, but enhanced release of vasopressin (VP) and its renal actions may be a possible mechanism. Excess secretion of VP in combination with large fluid ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:8:1:13
更新日期:1998-02-01 00:00:00
abstract::Computer analysis of the human placental lactogen-B (hPL-B) enhancer reveals two putative binding sites for the transcription factor NF-IL6, but the role of NF-IL6 in the regulation of the enhancer is unknown. Using gel mobility shift and supershift assays, we demonstrated that NF-IL6 binds to both enhancer sites. Tra...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:12:1:47
更新日期:2000-02-01 00:00:00
abstract::Thioredoxin-interacting protein (TXNIP) is a known important regulatory protein of islet β-cell biology and function, but the detailed mechanism is not clear. Autophagy plays a pivotal role in maintaining cellular homoeostasis. This study aimed to elucidate the influence of TXNIP on the autophagy of β-cell. In this st...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-020-02471-6
更新日期:2020-12-01 00:00:00
abstract::Steroid hormones regulate levels of gonadotropin mRNA in the pituitary, and gonadotropic hormones in plasma. To determine whether estrogen receptor alpha (ERalpha) mediates steroid negative feedback, wild type (WT) and estrogen receptor alpha knockout (ERalphaKO) mice of both sexes were gonadectomized and implanted wi...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:11:2:137
更新日期:1999-10-01 00:00:00
abstract::Previous evidence supports a role for growth hormone (GH)-insulin-like growth factor (IGF)-I deficiency in the pathophysiology of osteopenia/osteoporosis in adult thalassemia. Moreover, serum IGF-II has never been studied in this clinical condition. Thus, we elected to study the GH secretory status and the levels of c...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-016-0865-1
更新日期:2016-08-01 00:00:00
abstract::Leptin is an important hormone that has potent effects on appetite and body weight. The regulation of leptin gene expression and secretion by corticosteroids and insulin was studied in the rat. Adrenalectomy resulted in a significant reduction in leptin gene expression and secretion. The reduction was corrected by hor...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:8:1:85
更新日期:1998-02-01 00:00:00
abstract::Adrenal transplantation may restore adrenal function after bilateral adrenalectomy or when adrenal function is lost. Thus, animal experiments could provide useful information before clinical application of adrenal transplantation. Using an experimental model of autotransplantion of a complete adrenal gland in the sple...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/endo:16:3:173
更新日期:2001-12-01 00:00:00
abstract::Patients with ectopic ACTH syndrome often develop hypertension and hypokalemic alkalosis with an abnormal increase in the ratio of plasma cortisol to cortisone, indicating that 11 beta-hydroxysteroid dehydrogenase (11 beta HSD) activity is inhibited. Inhibition of 11 beta HSD allows access of cortisol or corticosteron...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02801327
更新日期:1997-12-01 00:00:00
abstract::Circadian rhythmicity is affected in obese subjects. This article analyzes the effect of a high-fat diet (35% fat) on 24-h changes circulating prolactin, luteinizing hormone (LH), testosterone, corticosterone, thyroid-stimulating hormone (TSH) and glucose, and pineal melatonin content, in rats. When body weight of rat...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-008-9066-x
更新日期:2008-04-01 00:00:00
abstract::Thyroid hormone (TH) abnormalities are common in patients with diabetes mellitus (DM). These thyroid hormone abnormalities have been associated with inflammatory activity in several conditions but this link remains unclear in DM. We assessed the influence of subclinical inflammation in TH metabolism in euthyroid diabe...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-015-0651-5
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:Hyponatremia is the most common electrolyte disorder in hospitalized patients, and its etiopathogenesis is related to an underlying tumor in 14% of cases. Hyponatremia has been associated with a worse outcome in several pathologies, including cancer, in which the leading cause of this electrolyte alteration is ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-019-02135-0
更新日期:2020-02-01 00:00:00
abstract::Fifty children ages 4-10 yr with type 1 diabetes mellitus volunteered to participate in a study to evaluate and compare a new needle-free device developed for growth hormone delivery. Children answered descriptive questions related to nervousness and worry, hurt or pain, redness or bleeding, and stinging and wetness. ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:15:1:015
更新日期:2001-06-01 00:00:00
abstract::The present study was designed to determine whether an association exists between HPA activity and cytokine production in major depression (MD). In 9 patients with MD and 11 control subjects of both sexes, all drug-free, activity of the HPA axis was evaluated by circadian rhythm of plasma cortisol, 24-h free urinary c...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-006-0016-1
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:Heparanase, galectin-3, and tissue factor (TF) are overexpressed in solid malignant thyroid tumors. We studied their expression in multinodular goiters (MNGs). DESIGN AND METHODS:Thyroid tissue specimens from 15 MNGs were obtained during surgery. mRNA expression for galectin-3, heparanase, and TF was assesse...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:22:2:81
更新日期:2003-11-01 00:00:00
abstract::Increasing evidence suggests that inflammatory response may be a critical component of different brain pathologies. However, the role played by this reaction is not fully understood. The present findings suggest that neuroinflammtory mediators such as cytokines may be involved in a number of key steps in the pathologi...
journal_title:Endocrine
pub_type: 杂志文章,评审
doi:10.1385/ENDO:29:2:243
更新日期:2006-04-01 00:00:00
abstract::A great number of prospective studies have investigated the relationship between leisure time physical activity (LTPA) and metabolic syndrome (MetS) risk. However, the results have been inconsistent. The aim of this study was to clarify the relationship between LTPA and MetS risk. Literature databases were searched in...
journal_title:Endocrine
pub_type: 杂志文章,meta分析
doi:10.1007/s12020-013-0110-0
更新日期:2014-06-01 00:00:00
abstract::This study estimates diagnostic performance of late-night salivary cortisol (LNSC) as measured by automated electrochemiluminescence immunoassay (ECLIA), evaluates the clinical implication of two consecutive LNSC measurements, and compares its accuracy with enzyme-linked immunosorbent assay (ELISA) and serum cortisol ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/s12020-012-9658-3
更新日期:2012-06-01 00:00:00