当前位置： SCI文献检索 > ENDOCRINE期刊下所有文献 > Thioredoxin-interacting protein: a critical link between autophagy disorders and pancreatic β-cell dysfunction.

Thioredoxin-interacting protein: a critical link between autophagy disorders and pancreatic β-cell dysfunction.

Abstract：

:Thioredoxin-interacting protein (TXNIP) is a known important regulatory protein of islet β-cell biology and function, but the detailed mechanism is not clear. Autophagy plays a pivotal role in maintaining cellular homoeostasis. This study aimed to elucidate the influence of TXNIP on the autophagy of β-cell. In this study, C57BL/6 mice and TXNIP-/- mice were fed with a standard diet (SD) or a high-fat and high-sugar diet (HFSD), and then we analysed biochemical and autophagy related indexes in the mice. We infected MIN6 cells with LV-TXNIP and siRNA TXNIP, then the cells were treated with free fatty acid (FFA), autophagic activator rapamycin (RAP), inhibitors of autophagy chloroquine (CQ) and bafilomycin A1(BAF), finally, we examined the changes of autophagy in MIN6 cells. The results showed that HFSD led to β-cell dysfunction and autophagy dysregulation, which was improved by TXNIP knockout in mice. In vitro experiments, TXNIP gene silencing enhanced LC3B-I conversion to LC3B-II, reduced the protein level of P62, decreased autophagosome accumulation induced by FFA treatment, increased the glucose-stimulated insulin secretion (GSIS) and autophagic flux inhibited by treatment with CQ. TXNIP overexpression induced upregulation of LC3B-I, LC3B-II and P62, accentuating the increase in autophagy and organelle destruction induced by FFA, and exacerbated the effect of BAF on the accumulation of autophagy proteins. Increasing TXNIP levels reduced GSIS, which was reversed by treatment with RAP. In summary, our study suggested that TXNIP is a critical link between autophagy disorders and pancreatic β-cell dysfunction.

journal_name

Endocrine

journal_title

Endocrine

authors

Deng W,Li Y,Ren Z,He Q,Jia Y,Liu Y,Zhang W,Gan X,Liu D

doi

10.1007/s12020-020-02471-6

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

526-537

issue

3

eissn

1355-008X

issn

1559-0100

pii

10.1007/s12020-020-02471-6

journal_volume

70

pub_type

杂志文章

相关文献

ENDOCRINE文献大全
  • Glucocorticoids regulate protein synthesis in hippocampal slices under mild heat shock conditions.

    abstract：:Glucocorticoid hormones potentiate the toxic effects of neuronal stressors. Alteration of gene expression by glucocorticoids could contribute to neuronal susceptibility by downregulating the synthesis of proteins necessary to adapt to challenge. Using heat shock of hippocampal slices as a model for cellular insult, pr...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1385/ENDO:8:2:135

    authors： Barr CS,Dokas LA

    更新日期：1998-04-01 00:00:00

  • Examination of the fasting and 2-h plasma glucose in the light of impairment in beta-cell function: what does the epidemiological data tell us?

    abstract：:We re-assess the fasting plasma glucose (FPG) and 2-h post-load glucose (2HPG) in the diagnosis of both prediabetes and type 2 diabetes mellitus by developing a gold standard based on beta-cell function. The gold standard was developed in a cohort of 2,152 adult subjects without severe renal or liver dysfunction that ...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-014-0284-0

    authors： Doi SA,Ward GM

    更新日期：2015-02-01 00:00:00

  • Correction to: Malignant struma ovarii: next-generation sequencing of six cases revealed Nras, Braf, and Jak3 mutations.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Endocrine

    pub_type： 已发布勘误

    doi：10.1007/s12020-020-02514-y

    authors： Poli R,Scatolini M,Grosso E,Maletta F,Gallo M,Liscia D,Nelva A,Cesario F,Forte G,Metovic J,Volante M,Arvat E,Papotti M

    更新日期：2020-12-01 00:00:00

  • Glucocorticoids curtail stimuli-induced CREB phosphorylation in TRH neurons through interaction of the glucocorticoid receptor with the catalytic subunit of protein kinase A.

    abstract：PURPOSE:Corticosterone prevents cold-induced stimulation of thyrotropin-releasing hormone (Trh) expression in rats, and the stimulatory effect of dibutyryl cyclic-adenosine monophosphate (dB-cAMP) on Trh transcription in hypothalamic cultures. We searched for the mechanism of this interference. METHODS:Immunohistochem...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-016-1223-z

    authors： Sotelo-Rivera I,Cote-Vélez A,Uribe RM,Charli JL,Joseph-Bravo P

    更新日期：2017-03-01 00:00:00

  • Risk profiles for metabolic syndrome and its transition patterns for the elderly in Beijing, 1992-2009.

    abstract：:There have been few reports on the development of metabolic disorders, especially when they are considered as a cluster. The purpose of this study was to describe risk profiles for metabolic syndrome (MetS) in elderly dwellers in Beijing, and to find their transition patterns over time. Data were derived from Beijing ...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-013-0143-4

    authors： Tao LX,Wang W,Zhu HP,Huo D,Zhou T,Pan L,Gao Q,Luo YX,Wu LJ,Li X,Tang Z,Guo XH

    更新日期：2014-09-01 00:00:00

  • Medullary thyroid carcinoma treated with percutaneous ultrasound-guided radiofrequency ablation.

    abstract：PURPOSE:Minimally invasive image-guided thermal ablation has been proposed as alternative to surgery for treatment of benign thyroid nodules and recurrent differentiated thyroid carcinoma. Here, we report for the first time the use of radiofrequency ablation (RFA) in a patient with non-metastatic medullary thyroid carc...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-019-01995-w

    authors： Biamonte E,Solbiati L,Ierace T,Colombo P,Lavezzi E,Mazziotti G,Lania A

    更新日期：2019-09-01 00:00:00

  • Neuropeptide Y and gamma-melanocyte stimulating hormone (gamma-MSH) share a common pressor mechanism of action.

    abstract：:Central circuits known to regulate food intake and energy expenditure also affect central cardiovascular regulation. For example, both the melanocortin and neuropeptide Y (NPY) peptide families, known to regulate food intake, also produce central hypertensive effects. Members of both families share a similar C-termina...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-008-9141-3

    authors： Gruber KA,Fan W,Akerberg H,Larhammar D,Chee MJ,Colmers WF,Cone RD

    更新日期：2009-06-01 00:00:00

  • Reference values for handgrip strength in young people of both sexes.

    abstract：:The handgrip strength is considered an excellent predictor of morbidity and mortality for acute and long term outcomes. In fact, several studies showed that the reduced handgrip strength is correlated to all-cause mortality in both middle aged and elderly subjects. Nevertheless, defined reference values of handgrip st...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-012-9733-9

    authors： Montalcini T,Migliaccio V,Yvelise F,Rotundo S,Mazza E,Liberato A,Pujia A

    更新日期：2013-04-01 00:00:00

  • Expression of membrane prostaglandin E synthase in human placenta and fetal membranes and effect of labor.

    abstract：:Initiation and maintenance of labor in humans is associated with an increase in prostaglandin synthesis by intrauterine tissues. The objective of the present study was to characterize the distribution of membrane-bound PGES (mPGES) protein and mPGES mRNA in human placenta, fetal membranes, and decidua at term and to d...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1385/ENDO:20:3:219

    authors： Alfaidy N,Sun M,Challis JR,Gibb W

    更新日期：2003-04-01 00:00:00

  • Complement action on secretory cells identified by the reverse hemolytic plaque assay: modified assay eliminates exposure of secretory cells to complement.

    abstract：:The application of a hemolytic plaque assay to antigen-secreting endocrine cells has brought about great advances in the study of regulation of hormone secretion. The reverse hemolytic plaque assay (RHPA) has enabled quantitation of secretion at the single cell level with simultaneous analysis of the population respon...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/BF03021421

    authors： Gregerson KA

    更新日期：1995-05-01 00:00:00

  • Modulatory effects of neurohypophysary origin hormones on placental hormone secretion at term.

    abstract：:Oxytocin (OT) and arginine-vasopressin (AVP) are two major neurohypophysary nonapeptides that have impor-tant roles during pregnancy and labor. Using the expiant model at term, we have examined the effect of physiologic concentrations of both nonapeptides on placental hormone secretion. We found that OT had a dose-dep...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/BF03021401

    authors： Barnea ER,Maruo T,Schurtz-Swirsky R

    更新日期：1995-04-01 00:00:00

  • Optimal timing of dopamine agonist withdrawal in patients with hyperprolactinemia: a systematic review and meta-analysis.

    abstract：PURPOSE:Dopamine agonists (DAs) are recommended as first-line treatment for patients with hyperprolactinemia. Generally, it is accepted that patients with hyperprolactinemia do not need lifelong medication, but the optimal timing for DA withdrawal has not been determined. The aim of this systematic review and meta-anal...

    journal_title：Endocrine

    pub_type： 杂志文章,meta分析,评审

    doi：10.1007/s12020-017-1444-9

    authors： Xia MY,Lou XH,Lin SJ,Wu ZB

    更新日期：2018-01-01 00:00:00

  • Effect of preproTRH antisense on thyrotropin-releasing hormone synthesis and viability of cultured rat diencephalic neurons.

    abstract：:To investigate a possible neurotropic role for thyrotropin-releasing hormone (TRH) in the central nervous system, we used recombinant antisense TRH adenovirus (TRHav) to "knock out" TRH in cultured 17-d fetal rat diencephalon. The morphology along with beta-galactosidase (beta-gal) enzyme histochemistry (X-gal stainin...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1385/ENDO:15:1:079

    authors： Luo LG,Lee SL,Lechan RM,Jackson IM

    更新日期：2001-06-01 00:00:00

  • Novel splicing variants of the human thyrotropin receptor encode truncated polypeptides without a membrane-spanning domain.

    abstract：:The thyrotropin receptor is of fundamental importance to normal thyroid function and is considered to be the predominant antigen affected by the autoantibodies of Graves' autoimmune hyperthyroidism. The identification of the epitopes on the receptor to which the autoantibodies bind or the mechanism by which the autoan...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/BF02994449

    authors： Hunt N,Willey KP,Abend N,Balvers M,Jähner D,Northemann W,Ivell R

    更新日期：1995-03-01 00:00:00

  • Galectin-3 and HBME-1 improve the accuracy of core biopsy in indeterminate thyroid nodules.

    abstract：:Core needle biopsy (CNB) has been recently described as an accurate second-line test in thyroid inconclusive cytology (FNA). Here we retrospectively investigated the potential improvement given by Galectin-3, Cytokeratin-19, and HBME-1 on the accuracy of CNB in thyroid nodules with prior indeterminate FNA report. The ...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-015-0678-7

    authors： Trimboli P,Guidobaldi L,Amendola S,Nasrollah N,Romanelli F,Attanasio D,Ramacciato G,Saggiorato E,Valabrega S,Crescenzi A

    更新日期：2016-04-01 00:00:00

  • Presence of ghrelin in normal and adenomatous human pituitary.

    abstract：:Recently, an endogenous ligand has been described for the growth hormone secretagogue receptor (GHS-R), named ghrelin. It was originally isolated from the stomach, but it is also present in the hypothalamus, where the highest concentration of GHS-R has been detected. It is well established that synthetic GHSs exert th...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1385/ENDO:14:1:101

    authors： Korbonits M,Kojima M,Kangawa K,Grossman AB

    更新日期：2001-02-01 00:00:00

  • Effects of incretin-based therapy in patients with heart failure and myocardial infarction.

    abstract：:Studies designed to evaluate the short-term effects of incretin-related drugs in subjects with cardiac disease are still preliminary. In patients with heart failure, two of five studies showed that glucagon-like peptide-1 (GLP-1) infusion was associated with an absolute increase in left ventricular ejection fraction (...

    journal_title：Endocrine

    pub_type： 杂志文章,评审

    doi：10.1007/s12020-014-0175-4

    authors： Mikhail N

    更新日期：2014-09-01 00:00:00

  • Osteoporosis and cardiovascular disease: brittle bones and boned arteries, is there a link?

    abstract：:Both osteoporosis and cardiovascular disease (CVD) are major public health problems leading to increased morbidity and mortality. Although traditionally viewed as separate disease entities that increase in prevalence with aging, accumulating evidence indicates that there are similar pathophysiological mechanisms under...

    journal_title：Endocrine

    pub_type： 杂志文章,评审

    doi：10.1385/ENDO:23:1:01

    authors： McFarlane SI,Muniyappa R,Shin JJ,Bahtiyar G,Sowers JR

    更新日期：2004-02-01 00:00:00

  • Effects of constant infusion with insulin-like growth factor-I (IGF-I) to immature female rats on body weight gain, tissue growth and sexual function. Evidence that such treatment does not affect sexual maturation of fertility.

    abstract：:Plasma levels for insulin-like growth factor-I (IGF-I) steadily increase in female rats between 20 and 40 d of life, and this increase is intimately related to the wellknown growth spurt occurring at this age. Since specific actions of IGF-I related to sexual function have been described at the ovarian and hypothalami...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：

    authors： Gruaz NM,d'Allèves V,Charnay Y,Skottner A,Ekvärn S,Fryklund L,Aubert ML

    更新日期：1997-02-01 00:00:00

  • Anti-Saccaromyces Cerevisiae antibodies (ASCA) are elevated in autoimmune thyroid disease ASCA in autoimmune thyroid disease.

    abstract：:Environmental factors have been implicated in the development of autoimmune thyroid disease (AITD). Anti-Saccaromyces Cerevisiae Antibodies (ASCA) were shown to be elevated in several autoimmune diseases. The aim of the study was to determine ASCA levels and their relationship with thyroid autoantibodies in patients w...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-010-9372-y

    authors： Yazıcı D,Aydın SZ,Yavuz D,Tarçın O,Deyneli O,Direskeneli H,Akalın S

    更新日期：2010-10-01 00:00:00

  • Gonadotropin-releasing hormone receptor gene expression in rat anterior pituitary.

    abstract：:Gonadotropin-releasing hormone (GnRH) effects on the lactotroph function have been widely studied, but they probably result from paracrine interactions. No visual data about GnRH receptor in the pituitary are available. In order to identify the GnRH target cells in the pituitary of adult rats, the cellular distributio...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/BF02782760

    authors： Mertani HC,Testart C,Ouhtit A,Brisson C,Morel G

    更新日期：1996-04-01 00:00:00

  • Prostaglandin F2α reduces steroidogenic acute regulatory (StAR) protein messenger ribonucleic acid expression in the rat ovary.

    abstract：:Steroid biosynthesis begins with the enzymatic conversion of cholesterol to pregnenolone. This reaction is catalyzed by the cytochrome P450 side-chain cleavage enzyme (P450scc), which is located on the matrix side of the inner mitochondrial membrane. Although the rate-limiting enzymatic step in steroidogenesis is the ...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/BF02738705

    authors： Sandhoff TW,McLean MP

    更新日期：1996-10-01 00:00:00

  • Hormonal induction of lordosis and ear wiggling in rat pups: gender and age differences.

    abstract：:To assess how early can estrogens induce female mating behaviors, rat pups 8-29 days old (D8-D29, respectively) were injected twice daily with estradiol benzoate (E) or oil (O) followed by progesterone (P) or oil, and then observed for the estrogen-dependent ear wiggling (EW) and lordosis in response to natural stimul...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-008-9046-1

    authors： Kow LM,Bogun M,Zhang Q,Pfaff DW

    更新日期：2007-12-01 00:00:00

  • Centrally administered urocortin 3 inhibits food intake and gastric emptying in mice.

    abstract：:Urocortin 3 (Ucn3) is recognized as a member of the corticotropin-releasing factor (CRF) family, which plays an important role in regulating food intake. We investigated the effects of centrally administered Ucn3 on food intake and gastric emptying in mice. Intracerebroventricular (ICV)administration of Ucn3 (0.1–1 nm...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-010-9420-7

    authors： Ushikai M,Asakawa A,Sakoguchi T,Tanaka C,Inui A

    更新日期：2011-04-01 00:00:00

  • The cAMP analogs have potent anti-proliferative effects on medullary thyroid cancer cell lines.

    abstract：:The oncogenic activation of the rearranged during transfection (RET) proto-oncogene has a main role in the pathogenesis of medullary thyroid cancer (MTC). Several lines of evidence suggest that RET function could be influenced by cyclic AMP (cAMP)-dependent protein kinase A (PKA) activity. We evaluated the in vitro an...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-015-0597-7

    authors： Dicitore A,Grassi ES,Caraglia M,Borghi MO,Gaudenzi G,Hofland LJ,Persani L,Vitale G

    更新日期：2016-01-01 00:00:00

  • Thyroid hormone acting via TRβ induces expression of browning genes in mouse bone marrow adipose tissue.

    abstract：PURPOSE:Mutant hypothyroid mouse models have recently shown that thyroid hormone is critical for skeletal development during an important prepubertal growth period. Additionally, thyroid hormone negatively regulates total body fat, consistent with the well-established effects of thyroid hormone on energy and fat metabo...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-017-1265-x

    authors： Lindsey RC,Mohan S

    更新日期：2017-04-01 00:00:00

  • Diurnal rhythmicity in breast-milk glucocorticoids, and infant behavior and sleep at age 3 months.

    abstract：PURPOSE:In previous studies, associations between breast-milk cortisol levels obtained on one occasion and infant neurodevelopment were demonstrated. However, more recent evidence indicates that breast-milk cortisol and cortisone concentrations follow the diurnal rhythm of maternal hypothalamus-pituitary-adrenal axis, ...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-020-02273-w

    authors： Toorop AA,van der Voorn B,Hollanders JJ,Dijkstra LR,Dolman KM,Heijboer AC,Rotteveel J,Honig A,Finken MJJ

    更新日期：2020-06-01 00:00:00

  • Oxidative stress in patients with thyroidectomy and thyroparathyroidectomy under replacement therapy.

    abstract：:Several studies have demonstrated an imbalance between free radicals and the antioxidative system in individuals with thyroid dysfunction. However, oxidative stress has not been evaluated in patients with thyroidectomy and thyroparathyroidectomy, who are under replacement therapy. The objective of this study was to ev...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-014-0270-6

    authors： Kaçmaz M,Atmaca M,Arslan A,Demir H,Özbay MF

    更新日期：2015-02-01 00:00:00

  • Analysis of differential gene expression by fiber-optic BeadArray and pathway in prolactinomas.

    abstract：:Prolactinomas are the most common secretory pituitary tumors; however, their pathogenesis is unclear. In order to explore the pathogenesis of prolactinomas, we used fiber-optic BeadArray to examine gene expression profiles in five prolactinomas compared with three normal pituitaries. Three down-regulated genes and one...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-010-9389-2

    authors： Jiang Z,Gui S,Zhang Y

    更新日期：2010-12-01 00:00:00

  • Variation analysis of anti-Müllerian hormone gene in Chinese women with polycystic ovary syndrome.

    abstract：PURPOSE:Anti-Müllerian hormone (AMH) is crucial for folliculogenesis. Prenatal exposure to AMH in mice produces a phenocopy of polycystic ovary syndrome (PCOS) in the adult female offspring. The aim of this study was to determine whether genetic variation in AMH gene contribute to PCOS in women of Chinese ancestry. ME...

    journal_title：Endocrine

    pub_type： 杂志文章

    doi：10.1007/s12020-020-02538-4

    authors： Qin L,Zhao S,Yang P,Cao Y,Zhang J,Chen ZJ,Dunaif A,Zhao H

    更新日期：2020-11-09 00:00:00