Abstract：
:A gram-positive, hexavalent chromium [chromate: Cr(VI)]-tolerant bacterium, isolated from tannery waste from Pakistan, was identified as a Microbacterium sp. by 16S rRNA gene sequence homology. The strain (designated as MP30) reduced toxic Cr(VI) only under anaerobic conditions at the expense of acetate as the electron donor. The bacterium was able to grow aerobically in L-broth supplemented with 15 mM CrO4(2-) but then did not reduce Cr(VI). At a concentration of 2.4x10(9) cells/ml, 100 microM sodium chromate was reduced within 30 h; however, the maximum specific reduction rate was obtained at lower initial cell concentrations.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Pattanapipitpaisal P,Brown NL,Macaskie LEdoi
10.1007/s002530100758subject
Has Abstractpub_date
2001-10-01 00:00:00pages
257-61issue
1-2eissn
0175-7598issn
1432-0614journal_volume
57pub_type
杂志文章abstract：:In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-ino...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF00166846
更新日期：1993-04-01 00:00:00
abstract：:Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA rev...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0347-0
更新日期：2006-10-01 00:00:00
abstract：:Production of volatile sulphur compounds (VSC) was assessed in culture media supplemented with L-methionine or L-methionine/L-cysteine mixtures, using five cheese-ripening yeasts: Debaryomyces hansenii DH47(8), Kluyveromyces lactis KL640, Geotrichum candidum GC77, Yarrowia lipolytica YL200 and Saccharomyces cerevisiae...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-007-0971-3
更新日期：2007-07-01 00:00:00
abstract：:Although acetate formation and tolerance are important criteria for various aspects of biotechnological process development, available studies on acetate tolerance in different species are disparate. We evaluate the response of eight bacterial strains, including two variants of Escherichia coli, two variants of Staphy...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530000339
更新日期：2000-08-01 00:00:00
abstract：:To understand the effect of daily bioaugmentation in full-scale grease interceptors (GIs), we compared the microbial communities occurring in two full-scale GIs during bioaugmented and non-bioaugmented cycles. The changes in microbial communities were determined using terminal restriction fragment length polymorphism ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7398-7
更新日期：2016-08-01 00:00:00
abstract：:A highly hydrophobic and non-toxic colloidal silica nanoparticle/polyvinyl butyral sol-gel composite membrane was prepared on a platinum wire electrode. With diphtheria-toxoid (D-Ag) as a model antigen and encapsulation of diphtheria antibody (D-Ab) in the composite architecture, this membrane could be used for reagen...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0353-2
更新日期：2006-10-01 00:00:00
abstract：:Clavulanic acid is a secondary metabolite produced by Streptomyces clavuligerus. It possesses a clavam structure and a characteristic 3R,5R stereochemistry essential for action as a beta-lactamase inhibitory molecule. It is produced from glyceraldehyde-3-phosphate and arginine in an eight step biosynthetic pathway. Th...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s002530000420
更新日期：2000-10-01 00:00:00
abstract：:Spillage of fossil-based oils during their conveyance through water conduits are sporadic, but significant environmental disasters. As the viscous hydrocarbons of the crude oils spread on water surface and choke aquatic life to death, their effective degradation is crucial for ecological balance. Though chemical and m...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-018-9434-2
更新日期：2019-01-01 00:00:00
abstract：:Microsomal fraction of fungal cells grabs the attention of many researchers for it contains enzymes that play a role in biotechnologically relevant processes. Microsomal enzymes, namely, CYP450s, were shown to metabolize a wide range of xenobiotic compounds, including PAHs, PCBs, dioxins, and endocrine disruptors, and...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-013-5347-2
更新日期：2013-12-01 00:00:00
abstract：:Bacillus stearothermophilus, a thermophilic bacterium isolated from the Kuwaiti desert, produced a variety of monohydroxy androstene derivatives and an oxidized product when incubated with exogenous testosterone for 24 h at 65 degrees C. The major metabolite was identified as androst-4-en-3,17-dione while minor metabo...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-003-1269-8
更新日期：2003-07-01 00:00:00
abstract：:Alkalophilic Bacillus sp. KSM-K16 produced three alkaline proteases, as detected by polyacrylamide gel electrophoresis (PAGE). The major protease, designated M protease, was recently purified to homogeneity and its properties were characterized. In the present study, two minor proteases, designated H protease and N pr...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530050650
更新日期：1996-03-01 00:00:00
abstract：:Aminotransferases (ATs) are important biocatalysts for the synthesis of chiral amines because of their capability of introducing amino group into ketones or keto acids as well as their high enantioselectivity, high regioselectivity. Among all ATs, branched-chain amino acid aminotransferase (BCAT) can use branched-chai...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-019-10105-9
更新日期：2019-10-01 00:00:00
abstract：:Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-8866-z
更新日期：2018-04-01 00:00:00
abstract：:The effect of sodium chloride (NaCl) on pullulan production by batch culture of Aureobasidium pullulans CCTCC M 2012259 was investigated. NaCl at 3 g/L improved the pullulan titer by 26.7% but reduced the molecular weight of pullulan to only 46.8% of that obtained in the control without NaCl. In order to elucidate the...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-9292-y
更新日期：2018-10-01 00:00:00
abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-008-1347-z
更新日期：2008-03-01 00:00:00
abstract：:By damaging DNA molecules, genotoxicants cause genetic mutations and also increase human susceptibility to cancers and genetic diseases. Over the past four decades, several assays have been developed in the budding yeast Saccharomyces cerevisiae to screen potential genotoxic substances and provide alternatives to anim...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-018-8783-1
更新日期：2018-03-01 00:00:00
abstract：:Streptomyces tendae Tü 901 produces the nucleoside peptide antibiotic nikkomycin. In shot-gun cloning experiments using pIJ699 as vector we isolated a 9.4-kb DNA fragment from S. tendae which complemented the nikkomycin nonproducing mutant NP9 to the formation of nikkomycin C/Cx and Kx. Nikkomycins were identified by ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF00903777
更新日期：1990-01-01 00:00:00
abstract：:1,3-1,4-beta-D-glucanase is an important endoglycosidase in the brewing and animal feed industries. To achieve high-level expression of recombinant glucanase in Pichia pastoris, we designed sequences encoding the alpha-factor signal peptide from Saccharomyces cerevisiae and the truncated 1,3-1,4-beta-D-glucanase from ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-007-1290-4
更新日期：2008-02-01 00:00:00
abstract：:Hyaluronic acid (HA) is a ubiquitous polysaccharide found in humans, animals, bacteria, algae and molluscs. Simple yet sophisticated, HA demonstrates unique and valuable rheological properties. In solution, HA behaves as a stiffened random coil and the resultant behaviour, even at low concentrations, is far from Newto...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-014-5853-x
更新日期：2014-08-01 00:00:00
abstract：:Several recent reports described an apoptosis-like programmed cell death (PCD) process in yeast in response to different environmental challenges. In this study, hyperosmotic stress caused by high NaCl concentration in culture medium induced cell death in the haploid yeast Torulopsis glabrata. Propidium iodide (PI) an...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-014-5589-7
更新日期：2014-04-01 00:00:00
abstract：:The temporal and spatial distribution of the genus Bacillus and Clostridium histolyticum group in swine manure composting was determined by fluorescent in situ hybridization using fluorescently labeled 16S rRNA-targeted oligonucleotide probes LGC353b and Chis150, respectively. The temporal distribution of total bacter...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3558-y
更新日期：2012-03-01 00:00:00
abstract：:Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-9347-0
更新日期：2018-11-01 00:00:00
abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3776-3
更新日期：2012-03-01 00:00:00
abstract：:Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair,...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-013-4935-5
更新日期：2013-09-01 00:00:00
abstract：:Mycorrhizal fungi belonging to the genus Tuber produce, after the establishment of a productive interaction with a plant host, hypogeous fruitbodies of great economic value known as ''truffles''. This review summarizes the state of art on life cycle, genetic, and biotechnological investigations of Tuber spp. The ascoc...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-006-0519-y
更新日期：2006-09-01 00:00:00
abstract：:Vinegar is a traditional fermented condiment, and the microbial diversity of DaQu makes the quality of vinegar products. Recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. In this study, the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar was analyz...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-014-5697-4
更新日期：2014-07-01 00:00:00
abstract：:Endophytic actinobacteria, which exist in the inner tissues of living plants, have attracted increasing attention among taxonomists, ecologists, agronomists, chemists and evolutionary biologists. Numerous studies have indicated that these prolific actinobacteria appear to have a capacity to produce an impressive array...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-010-2923-6
更新日期：2011-02-01 00:00:00
abstract：:The influence of different amino acid substitutions in the nitrilase from Pseudomonas fluorescens EBC191 (NitA) on the catalytical activity and the ability to form amides was investigated. The enzyme variant Glu137Ala was constructed because glutamate residues homologous to Glu137 are highly conserved among different ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-014-6061-4
更新日期：2015-03-01 00:00:00
abstract：:The extrusion technology of blends formed by compounds with different physicochemical properties often results in new materials that present properties distinctive from its original individual constituents. Here, we report the use of melt extrusion of blends made from low-cost materials to produce a biodegradable foam...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7566-9
更新日期：2016-08-01 00:00:00
abstract：:Grapevines are sensitive to a wide range of fungal pathogens, including agents such as Phaeomoniella chlamydospora and Phaeoacremonium aleophilum that cause tracheomycosis. In the present study, a procedure for DNA extraction from grapevine woody tissue is first evaluated and shown to be suitable for quantitative anal...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-013-5299-6
更新日期：2013-12-01 00:00:00
