Abstract:
:Integrins play key roles in cell-to-cell and cell-to-extracellular matrix (ECM) adhesion. We investigated integrin expression on pleural mesothelial cells (PMCs) and the inhibitory effect of arginine-glycine-asparate (RGD)-containing peptide on the adhesion of PMCs to fibronectin and collagen. Using flow cytometry and immunostaining, PMCs freshly isolated from pleural effusions and one mesothelial cell line were screened for different integrins. Intact pleural tissue was evaluated by immunohistochemistry. The adhesion of Met-5A cells to fibronectin and collagen types I, III and IV was assayed with prior treatment of various concentrations of glycine-arginine-glycine aspartate-serine (GRGDS). On primary PMCs, alpha2, alpha3, alpha5, beta1, beta3 and alphavbeta3 were highly expressed (>70%); alpha1 expression was intermediate (30-70%); and alpha4 and alpha6 expressions were low (< 30%). On Met-SA cells, alpha3, alpha5, alpha6 and beta1 were highly expressed (>70%); alpha1 was intermediate (30-70%); and alpha2, alpha4, beta3 and alphavbeta3 were low (<30%). The patterns of immunostaining on pleural tissues were similar to the results of flow cytometry for primary PMCs except for beta3. There was no statistically different expression in various disease states (transudate vs. exudate, benign vs. malignant). The inhibitory effect of GRGDS peptide on Met-5A cell adhesion to all four matrix proteins was dose-dependent.
journal_name
Respir Medjournal_title
Respiratory medicineauthors
Liaw YS,Yu CJ,Shun CT,Lee YC,Kuo SH,Luh KT,Yang PCdoi
10.1053/rmed.2000.1026subject
Has Abstractpub_date
2001-03-01 00:00:00pages
221-6issue
3eissn
0954-6111issn
1532-3064pii
S0954-6111(00)91026-6journal_volume
95pub_type
杂志文章abstract::A multiplex polymerase chain reaction (PCR) was developed for the simultaneous detection of Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella pneumophila. Oligonucleotide primers for the amplification of the DNA of these three organisms were optimized for use in combination in the same reaction. PCR products ...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2003.11.012
更新日期:2004-06-01 00:00:00
abstract:UNLABELLED:We report bronchoscopic changes observed in children with recurrent lower airways infections (RLAI) and findings in control children undergoing bronchoscopy for causes other than RLAI. PATIENTS AND METHODS:Retrospective case-control cohorts study. The clinical records of children who had fiberoptic bronchos...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2014.02.014
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:Asthma in the elderly as well as asthma of adult-onset has been associated with increased morbidity, but little is known specifically about the effects of age on clinical and inflammatory outcomes in severe refractory asthma. The aims of the study were to examine the effects of age [<65 versus ≥65 years] and...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2016.07.005
更新日期:2016-09-01 00:00:00
abstract::The aim of this study was to compare the efficacy and safety of salmeterol/fluticasone propionate combination product (SFC) with fluticasone propionate (FP) plus oral montelukast (M) over 12 weeks in symptomatic asthma patients. The study was a multinational, randomised, double-blind, double-dummy, parallel-group desi...
journal_title:Respiratory medicine
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1053/rmed.2003.1436
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Recent studies have shown strong evidence that bronchiolitis obliterans is the major long-term sequelae of exposure to sulfur mustard (SM). This study is the first to examine the histopathologic spectrum of changes in a large number of surgical lung biopsies from patients exposed to SM. METHOD:Fifteen patie...
journal_title:Respiratory medicine
pub_type: 杂志文章,多中心研究
doi:10.1016/j.rmed.2008.01.016
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVES:Influenza and Influenza-like syndromes (I-LSs) are very common events in general practice, and their relevance is frequently underestimated. Aim of the study was to assess the economic impact of influenza and Influenza-like syndromes (I-LSs) in the Italian general population by using real-world data from a r...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2018.07.001
更新日期:2018-08-01 00:00:00
abstract::The objective of this study was to evaluate the effect of lateral body position on gas exchange in patients with unilateral pleural effusion, with special reference to the influence of effusion volume. Thirty consecutive patients with unilateral pleural effusion, without evidence of parenchymal pulmonary involvement, ...
journal_title:Respiratory medicine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0954-6111(95)90091-8
更新日期:1995-04-01 00:00:00
abstract:BACKGROUND:A clinical stability (CS) evaluation is thought to be important in community-acquired pneumonia (CAP) treatment, but evidence concerning the time to CS (TCS) remains lacking. METHODS:Among consecutive patients hospitalized with pneumococcal pneumonia, relationships between TCS and other clinical outcomes we...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2014.02.007
更新日期:2014-05-01 00:00:00
abstract::Antifibrotic drugs for idiopathic pulmonary fibrosis patients in England and Scotland are only available to those with FVC percent predicted (FVC%pred) less than or equal to 80%. The prescribing guidance does not state which set of reference values should be used and we show that a patient's FVC%pred can change by 4-6...
journal_title:Respiratory medicine
pub_type: 信件
doi:10.1016/j.rmed.2016.10.007
更新日期:2016-11-01 00:00:00
abstract:STUDY OBJECTIVES:The process of intermittent hypoxia-reoxygenation produces airway inflammation and endothelial dysfunction that favors the development of cardiovascular disorders in obstructive sleep apnea syndrome (OSAS). Nitric oxide (NO) is an important mediator in airway inflammation and the regulation of endothel...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2010.12.004
更新日期:2011-04-01 00:00:00
abstract::Surfactant protein-A enhances the phagocytosis and killing of many pathogens, although studying this effect in an assortment of models and different experimental protocols has sometimes yielded conflicting results. In this report, using the human THP-1 cell line as the primary phagocytic cell, we systematically examin...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2003.12.018
更新日期:2004-07-01 00:00:00
abstract:INTRODUCTION:The aim was to investigate temporal variation in Health-Related Quality of Life (HRQL) and factors influencing low HRQL, in patients with asthma. MATERIAL AND METHODS:Questionnaire data on patient characteristics and the mini-Asthma Quality of Life Questionnaire (mini-AQLQ) scores from two separate cohort...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2017.10.010
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:Tracheal collapse is a weakness of the tracheal wall leading to expiratory central airway collapse of more than 50% compared to inspiration. It has previously been discussed whether the collapsibility of the greater airways is a stable or a dynamic condition. Indeed, other well-known lung diseases such as as...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2018.11.018
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Obstructive sleep apnea syndrome is associated with airway inflammation. Measurement of exhaled nitric oxide is a non-invasive method for evaluation of airway diseases. It seems that obesity is an exacerbating factor for airway inflammation. We aimed to evaluate the changes of exhaled nitric oxide after slee...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2014.05.010
更新日期:2014-08-01 00:00:00
abstract::Previous studies have shown that chronic hypoxia leads to changes in skeletal muscle structure (fibre size and type) and activities of several bioenergetic enzymes. Whether this occurs also in conditions characterised by intermittent hypoxia, such as the obstructive sleep apnoea syndrome (OSAS), is unknown. To explore...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/s0954-6111(03)00034-9
更新日期:2003-07-01 00:00:00
abstract:BACKGROUND:Idiopathic pleuroparenchymal fibroelastosis (IPPFE) is a rare idiopathic interstitial pneumonia characterized by an upper lobe-dominant interstitial increase in predominantly elastic fibers. The accumulation of cases has resulted in a refinement of the disease concept, but there are no blood biomarkers to ai...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2020.106077
更新日期:2020-09-01 00:00:00
abstract::The marked female predominance in cases of idiopathic chronic cough and its association with mild chronic lymphocytic airway inflammation suggests an underlying autoimmune process. We set out to test the hypothesis that idiopathic chronic cough is associated with other organ-specific autoimmune diseases in a case cont...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2003.10.005
更新日期:2004-03-01 00:00:00
abstract::Epithelioid haemangioendothelioma of the pulmonary vessels is a rare neoplasm which usually has a minimal clinical expression. The present case report describes an exceptional case with severe inaugural alveolar haemorrhage which led to death by acute respiratory failure within a few weeks. ...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/s0954-6111(97)90077-9
更新日期:1997-02-01 00:00:00
abstract::The Spacehaler is a new, compact, pressurized aerosol device that uses the same canister as a conventional metered-dose inhaler (MDI). Its design, however, reduces the velocity of the aerosol cloud that emerges from the inhaler, thereby reducing the amount of the non-respirable fraction of the drug delivered to the pa...
journal_title:Respiratory medicine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0954-6111(97)90035-4
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:The choice of inhaler device for asthma patients depends upon multiple attributes. We investigated factors that may drive general practitioners (GPs) and respiratory specialists in the prescription of inhaler devices for asthma patients who initiated inhalation therapy. METHODS:We retrospectively analysed p...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2020.105937
更新日期:2020-05-01 00:00:00
abstract:INTRODUCTION:Up to 40% of patients seen in a cough clinic have unexplained chronic cough. The long term outcome of these patients is uncertain. OBJECTIVE:To determine the long-term outcome in patients diagnosed with unexplained chronic cough. METHODS:We have performed a longitudinal study of symptoms, airway inflamma...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2012.11.018
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:Patients with Chronic Obstructive Pulmonary Disease with chronic respiratory failure (COPD-CRF) experience high mortality and healthcare utilization. Non-invasive home ventilation (NIVH) is increasingly used in such patients. We examined the associations between NIVH and survival, hospitalizations, and emerg...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2020.106291
更新日期:2020-12-30 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2015.04.014
更新日期:2015-08-01 00:00:00
abstract::Gemcitabine is a newer pyrimidine analog used for the treatment of solid tumors. Though generally considered safe, it can cause pulmonary toxicity, which is generally mild and reversible. The purpose of this publication is to document a case of adult respiratory distress syndrome (ARDS) caused by Gemcitabine administr...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2005.08.002
更新日期:2006-04-01 00:00:00
abstract:UNLABELLED:Misdiagnoses are inevitable when working hypotheses of asthma/COPD of General Practitioners (GPs) are not checked by spirometry. To reduce misdiagnoses, Asthma/COPD-support services (AC-services) offer support by performing spirometry assessed together with written medical history by consulting pulmonologist...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2012.03.002
更新日期:2012-08-01 00:00:00
abstract::Current asthma management guidelines state that where a patient is receiving a low to moderate dose of inhaled corticosteroids and is still experiencing symptoms the dose of corticosteroid should be increased and, if necessary, a long-acting bronchodilator should be added. Many studies have now shown that the addition...
journal_title:Respiratory medicine
pub_type: 杂志文章,meta分析
doi:10.1053/rmed.2001.1140
更新日期:2001-08-01 00:00:00
abstract:BACKGROUND:The current literature suggests a relationship between obstructive sleep apnea (OSA) severity and hematocrit. However, the degree that OSA contributes to clinically significant erythrocytosis is uncertain. The aim of this study is to evaluate this association in a large study sample controlling for multiple ...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2017.07.003
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:We aimed to compare chest low-dose computed tomography (LDCT) with chest radiography (CXR) in the assessment of febrile acute myeloid leukaemia neutropenic patients. METHODS:A prospective non-randomized study was carried out between 30 May, 2003 and 3 June, 2004 in consecutive neutropenic patients who requi...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2009.11.003
更新日期:2010-04-01 00:00:00
abstract::We conducted a large population-based retrospective cross-sectional study for determining the extent of clinically recognized chronic obstructive pulmonary disease (COPD) and asthma, and the prevalence of associated cardiovascular diseases (CVDs), using information obtained from the Health Search Database (HSD) owned ...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2011.07.021
更新日期:2012-02-01 00:00:00
abstract::Nasal mucociliary system is the first line of defense of the upper airways and may be affected acutely by exposure to particulate matter (PM) from biomass burning. Several epidemiologic studies have demonstrated a consistent association between levels of air pollution from biomass burning with increases in hospitaliza...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2011.08.003
更新日期:2011-11-01 00:00:00