Abstract:
:In mammals, skeletal muscle mass is negatively regulated by a muscle-derived growth/differentiating factor named myostatin (MSTN) that belongs to the transforming growth factor-beta superfamily. Although putative MSTN homologs have been identified from several vertebrates, nonmammalian orthologs remained poorly defined. Thus, we isolated and characterized MSTN complementary DNA clones from the skeletal muscle of the tilapia Oreochromis mossambicus and the white bass Morone chrysops. The nucleic and amino acid sequences from both fish species are highly homologous to the previously identified mammalian and avian orthologs, and both possess conserved cysteine residues and putative RXXR proteolytic processing sites that are common to all transforming growth factor-beta family members. Western blotting of conditioned medium from human embryonal kidney (HEK293) cells overexpressing a His-tagged tilapia MSTN indicates that the secreted fish protein is processed in a manner similar to mouse MSTN. However, in contrast to mice, MSTN expression in tilapia is not limited to skeletal muscle as it occurs in many tissues. Furthermore, the timing of MSTN expression in developing tilapia larvae coincides with myogenesis. These results suggest that the biological actions of MSTN in the tilapia and possibly in other fishes may not be limited to myocyte growth repression, but may additionally influence different cell types and organ systems.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Rodgers BD,Weber GM,Sullivan CV,Levine MAdoi
10.1210/endo.142.4.8097subject
Has Abstractpub_date
2001-04-01 00:00:00pages
1412-8issue
4eissn
0013-7227issn
1945-7170journal_volume
142pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The hepatic extractions of gastric inhibitory polypeptide (GIP) and insulin were determined using in vitro and in vivo methods to assess the role of the liver in GIP metabolism and the possible effect of GIP on the hepatic extraction of insulin. During in vitro studies using the isolated perfused rat liver, infusion o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-1011
更新日期:1984-09-01 00:00:00
abstract::Insulin-like growth factor-I (IGF-I), the principal IGF in adult rat serum, occurs complexed to specific binding proteins. After fractionation of serum on Sephadex G-200 at neutral pH, 62% of the immunoreactive IGF-I is recovered in the 150K region, 38% in the 40K region, and none is present as free 7.5K IGF-I. Adult ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1540
更新日期:1989-09-01 00:00:00
abstract::Chemerin is an adipokine with important regulatory roles in adipogenesis. In humans, serum total chemerin (i.e. prochemerin plus chemerin) levels are positively associated with body mass index and metabolic syndrome. However, the mechanisms that increase serum chemerin concentration are unknown. We hypothesized that c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0794
更新日期:2010-06-01 00:00:00
abstract::Angiotensin II is a potent octapeptide vasoconstrictor and regulator of cardiovascular and electrolyte homeostasis. Angiotensinogen, the protein precursor of angiotensin II, is synthesized by the liver and many other organs of the adult rat. To determine whether angiotensin may be present in early fetal development we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-4-1335
更新日期:1987-10-01 00:00:00
abstract::We present a map describing the antigenic determinants (epitopes) of the human CG (hCG) molecule. A panel of monoclonal antibodies to hCG, previously characterized by us was incorporated into two-site binding assays to probe hCG by a 21 X 11 matrix of 231 pairs of MCA. Nine different reaction patterns of reciprocal ho...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-189
更新日期:1986-01-01 00:00:00
abstract::Steroid hormones can differentially modify gonadotropin release stimulated by GnRH. Decreased GnRH-mediated gonadotropin release has been observed in vitro after pretreatment with androgens or glucocorticoids. In this study, we tested this phenomenon further with the use of a new cytochemical stain for a potent biotin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-1-396
更新日期:1985-07-01 00:00:00
abstract::We investigated the effects of thyroid status on nitric oxide synthase (NOS) gene expression in the rat hypothalamic paraventricular (PVN) and supraoptic nuclei (SON). Propylthiouracil (PTU)-induced hypothyroidism in male rats produced a highly significant reduction in NOS gene transcripts in the PVN and SON, as asses...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7545100
更新日期:1995-10-01 00:00:00
abstract::We have developed a highly sensitive and specific RIA for propylthiouracil (PTU) which uses 125I-labeled PTU as the radioactive ligand. At a final antibody dilution of 1:10,000, the detection limit for PTU was 100 pg; cross-reactivity with circulating, urinary, and intrathyroid PTU metabolites was negligible. Using th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-3-915
更新日期:1983-09-01 00:00:00
abstract::In order to study whether changes in adenohypophysial dopamine are only related to PRL release or also to the variation of LH or FSH, dopamine (DA) in the pars distalis was measured during the estrous cycle and after castration of both male and female rats. Catecholamines were assayed using a highly sensitive radioenz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-6-1682
更新日期:1980-06-01 00:00:00
abstract::Little is known about the mechanisms of action of polypeptide hormones on chromatin structure and nuclear function. We have employed micrococcal nuclease to examine the effect of TSH on the accessibility of DNA in thyroid nuclei. Brief digestion of nuclear suspensions with 0.05-0.2 U/ml micrococcal nuclease at 26-28 C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-6-2118
更新日期:1982-06-01 00:00:00
abstract::Levels of bovine placental lactogen (bPL) have been measured in the serum of dairy and beef cattle and in the milk and amniotic fluid of pregnant animals with a highly specific radioimmunoassay. In both dairy and beef cows, serum bPL levels remain low (less than 50 ng/ml) during the first two trimesters and then rise ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-5-1273
更新日期:1976-11-01 00:00:00
abstract::Increased toxicant exposure and resultant environmentally induced diseases are a tradeoff of industrial productivity. Dioxin [2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD)], a ubiquitous byproduct, is associated with a spectrum of diseases including endometriosis, a common, chronic disease in women. TCDD activates cytoch...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00860
更新日期:2018-01-01 00:00:00
abstract::The mechanism of the hypophosphatemic effect of thyrocalcitonin (TCT) is uncertain and may involve removal of inorganic phosphate (P1) from the plasma by heretofore unidentified organs. Rats, 192 g. thyroparathyroidectomized 16-24 h earlier, received 100 MRC mU TCT or vehicle (V) SC and 100 muCi 32PO4 iv. One h later ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-4-1048
更新日期:1975-04-01 00:00:00
abstract::Hexose-6-phosphate dehydrogenase (EC 1.1.1.47) catalyzes the conversion of glucose 6-phosphate to 6-phosphogluconolactone within the lumen of the endoplasmic reticulum, thereby generating reduced nicotinamide adenine dinucleotide phosphate. Reduced nicotinamide adenine dinucleotide phosphate is a necessary cofactor fo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0593
更新日期:2007-10-01 00:00:00
abstract::The rat S14 gene is synergistically regulated by thyroid hormone and carbohydrates. The 5'-flanking region of this gene contains both carbohydrate and thyroid hormone response elements (TREs). We recently located the carbohydrate response element (CHORE) between -1583 and -1069 bases from the start site of transcripti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8319560
更新日期:1993-07-01 00:00:00
abstract::We have recently found that, in the frog adrenal gland, endozepines are present in chromaffin cells and we have shown that the triakontatetraneuropeptide TTN is a potent stimulator of corticosteroid secretion in vitro. In the present study, we have investigated the transduction mechanisms mediating the corticotropic e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.1.7228
更新日期:2000-01-01 00:00:00
abstract::To define the specific role of IGF-I receptor (IGF-IR) in adipogenic and thermogenic differentiation of brown adipocytes during late fetal life, we have established immortalized brown adipocyte cell lines from fetuses of IGF-IR-deficient mice (IGF-IR(-/-)) as well as from wild-type mice (IGF-IR(+/+)). IGF-IR(-/-) cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220828
更新日期:2003-02-01 00:00:00
abstract::Fasting in rodents is characterized by decreases in serum T4 and T3 levels but no compensatory increase in serum TSH level. The types 1 and 2 deiodinases (D1 and D2) are postulated to play key roles in mediating these changes. However, serum T4 and T3 levels in fasted 5'-deiodinase-deficient mice decreased by at least...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1884
更新日期:2014-08-01 00:00:00
abstract::There is now general agreement that neurokinin B (NKB) acts via neurokinin-3-receptor (NK3R) to stimulate secretion of GnRH and LH in several species, including rats, mice, sheep, and humans. However, the roles of two other tachykinins, substance P (SP) and neurokinin A, which act primarily via NK1R and NK2R, respecti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1565
更新日期:2016-12-01 00:00:00
abstract::Poor growth before birth increases the risk of non-insulin-dependent diabetes mellitus (NIDDM) and impairs insulin secretion relative to sensitivity. We investigated the effects of intrauterine growth restriction in sheep on insulin secretion, beta-cell mass, and function from before birth to young adulthood and its m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0233
更新日期:2008-10-01 00:00:00
abstract::The actions of IGF-I and IGF-II are thought to be largely due to their activation of the IGF-I receptor. However, IGF-II can also bind with high affinity to, and effectively activate, an isoform of the insulin receptor (IR-A) that lacks a sequence at the carboxyl-terminal end of the extracellular alpha subunit due to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0736
更新日期:2006-02-01 00:00:00
abstract::Skeletal cells synthesize bone morphogenetic proteins (BMPs) and BMP antagonists. Gremlin, a BMP antagonist, is expressed in osteoblasts and opposes BMP effects on osteoblastic differentiation and function in vitro. However, its effects in vivo are not known. To investigate the actions of gremlin on bone remodeling in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0766
更新日期:2005-02-01 00:00:00
abstract::PTH upregulates the expression of the receptor activator of nuclear factor κB ligand (Rankl) in cells of the osteoblastic lineage, but the precise differentiation stage of the PTH target cell responsible for RANKL-mediated stimulation of bone resorption remains undefined. We report that constitutive activation of PTH ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1046
更新日期:2014-08-01 00:00:00
abstract::Altered leptin action has been implicated in the pathophysiology of heart failure in obesity, a hallmark of which is extracellular matrix remodeling. Here, we characterize the direct influence of leptin on matrix metalloproteinase (MMP) activity in primary adult rat cardiac fibroblasts and focus on elucidating the mol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1166
更新日期:2011-05-01 00:00:00
abstract::Nutritional programming, taking place in utero or early after birth, is closely linked with metabolic and appetite disorders in adulthood. Following the hypothesis that nutritional programming impacts hypothalamic neuronal organization, we report on discrepancies of multiple molecular and cellular early events that ta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0893
更新日期:2010-02-01 00:00:00
abstract::A major cause of infertility in normal and assisted reproduction cycles is failure of the endometrium to undergo appropriate changes during the secretory phase of the menstrual cycle as it acquires receptivity for an implanting blastocyst. Current dogma states that loss of epithelial polarity in the luminal epithelial...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1877
更新日期:2018-01-01 00:00:00
abstract::GH is known to increase the formation of bone and hard tissues of the tooth (dentine, cementum, and enamel), as do bone morphogenetic proteins. GH receptors are expressed in these tissues and could mediate local growth responses. Here we report that both GH and insulin-like growth factor I (IGF-I) are able to increase...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.9.6211
更新日期:1998-09-01 00:00:00
abstract::The ability to respond to variations in nutritional status depends on regulatory systems that monitor nutrient intake and adaptively alter metabolism and feeding behavior during nutrient restriction. There is ample evidence that the restriction of water, sodium, or energy intake triggers adaptive responses that conser...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqaa019
更新日期:2020-03-01 00:00:00
abstract::In previous studies we found that plasma angiotensinogen levels were reduced by lesions of the hypothalamic paraventricular nuclei. To determine if the decrease was caused by decreased secretion of hormones that normally stimulate angiotensinogen secretion by the liver, we correlated the changes in plasma angiotensino...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-901
更新日期:1991-08-01 00:00:00
abstract::The genetically obese Zucker rat is a widely used model of early-onset obesity. Like obese children, these obese rats are hyperinsulinemic and have low GH secretion. However, data on linear growth and insulin-like growth factor-I (IGF-I) levels in this model are scanty and contradictory. In the present study, we inves...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.7509740
更新日期:1994-03-01 00:00:00