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On the equilibrium between monomeric alpha-lactalbumin and the chaperoning complex of alpha-crystallin.

Abstract：

:In chaperoning dithiothreitol-denatured alpha-lactabumin, alpha-crystallin forms a chaperoning complex. In order to study the kinetics of such chaperoning it needs to be established whether the formation of the chaperoning complex is a reversible or irreversible process. The chaperoning reaction was studied by dynamic light scattering as a function of concentration and weight ratio of alpha-lactalbumin/alpha-crystallin. HPLC and subsequent SDS-PAGE gel electrophoresis experiments established that the chaperoning complex formed contains both alpha-crystallin and alpha-lactalbumin. Upon rechromatographing the chaperoning complex, the presence of monomeric alpha-lactalbumin has been demonstrated in addition to the chaperoning complex itself. This and equilibrium dialysis experiments demonstrated conclusively the existence of an equilibrium between monomeric partially denatured alpha-lactalbumin and the chaperoning complex made of alpha-lactalbumin and alpha-crystallin.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Neal R,Zigler JS Jr,Bettelheim FA

doi

10.1006/bbrc.2000.4092

subject

Has Abstract

pub_date

2001-01-12 00:00:00

pages

14-8

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(00)94092-0

journal_volume

280

pub_type

杂志文章

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