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Efficient in vitro transduction of naive murine B cells with lentiviral vectors.

Abstract：

:The aim of this study was to determine the impact of lentiviral transduction on primary murine B cells. Studying B cell activities in vivo or using them for tolerance induction requires that the cells remain unaltered in their biological behavior except for expression of the transgene. As we show here, murine B cells can efficiently be transduced by lentiviral, VSV-G-pseudotyped vectors without the necessity of prior activation. Culture with LPS gave enhanced transduction efficiencies but led to the upregulation of CD86 and proliferation of the cells. Transduction of naive B cells by lentiviral vectors was dependent on multiplicity of infection and did not lead to a concomitant activation. Furthermore, the transduced cells could be used for studies in the NOD mouse system without altering the onset of diabetes. We conclude that lentiviral gene transfer into naive B cells is a powerful tool for manipulation of B cells for therapeutic applications.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Warncke M,Vogt B,Ulrich J,von Laer MD,Beyer W,Klump H,Micheel B,Sheriff A

doi

10.1016/j.bbrc.2004.04.057

subject

Has Abstract

pub_date

2004-06-04 00:00:00

pages

673-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X04007831

journal_volume

318

pub_type

杂志文章

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