Abstract：
:A general strategy is described for using the cleavage site of restriction enzymes in vectors for cloning regardless of how many sites the given enzymes have in the vector. The application of this method allows one to open any vector at its cloning site with protruding ends which can be compatible with almost every commercially available Class II restriction enzyme. By employing this method, the laborious construction of new vectors can be simplified considerably. This general strategy is based on the known ability of Class IIS restriction enzymes to cut any sequence located outside of their recognition site; the introduction of a linker containing recognition site(s) for Class IIS restriction enzyme(s), not present originally in the vector, gives rise to the possibility of opening the vector so as to produce overhangs of arbitrary sequence. In particular, when a symmetrical short sequence representing the protruding end of any Class II enzyme is situated at the cutting position of the Class IIS enzyme, cleavage with the Class IIS enzyme exposes the hitherto hidden, "unique" cloning site. This technique is demonstrated by cloning the cDNA of the multidrug resistance protein to an expression vector.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Welker E,Váradi Adoi
10.1006/bbrc.2000.2668subject
Has Abstractpub_date
2000-05-10 00:00:00pages
534-6issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(00)92668-8journal_volume
271pub_type
杂志文章abstract：:Transcription factors, such as PDX-1, that normally mediate pancreatic development are capable of inducing hepatic progenitor cells to differentiate into cells with pancreatic islet characteristics. We hypothesized that simultaneous expression of multiple transcription factors involved in islet development might enhan...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2006.12.216
更新日期：2007-03-09 00:00:00
abstract：:Amyloid fibrils were isolated from amyloid-laden tissue obtained from a chronic hemodialysis patient with carpal tunnel syndrome. After solubilization in guanidine HCl, a significant amount of the protein was located in a homogeneous low molecular weight fraction. The protein was found to be identical to beta 2-microg...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(85)91948-5
更新日期：1985-06-28 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(85)91817-0
更新日期：1985-08-30 00:00:00
abstract：:The tumor suppressor p53 is involved in cadmium (Cd)-induced apoptosis and autophagy. However, the regulatory mechanisms of p53 in Cd-induced kidney injury are not well established. Here, we report the role of autophagy in Cd-induced p53 induction in human proximal tubular cells (HK-2). HK-2 cells treated with Cd indu...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2020.12.019
更新日期：2021-01-01 00:00:00
abstract：:Cep169 is a centrosomal protein conserved among vertebrates. In our previous reports, we showed that mammalian Cep169 interacts and collaborates with CDK5RAP2 to regulate microtubule (MT) dynamics and stabilization. Although Cep169 is required for MT regulation, its precise cellular function remains largely elusive. H...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2015.11.004
更新日期：2015-12-25 00:00:00
abstract：:Temozolomide (TMZ) is a methylating agent used in chemotherapy against glioblastoma. This work was designed to clarify details in repair pathways acting to remove DNA double-strand breaks (DSBs) induced by TMZ. Cultured mouse embryonic fibroblasts were used which were deficient in DSB repair genes such as homologous r...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2009.07.045
更新日期：2009-10-02 00:00:00
abstract：:N-Palmitoyl-(S)-seryl-(S)-seryl-(S)-asparaginyl-(S)-alanine (Palmitoyl-tetrapeptide) is an analogue of the N-terminal part of the lipoprotein from the outer membrane of Escherichia coli. It was prepared by chemical synthesis and tested for biological activity in in vitro lymphocyte culture systems. In spleen cells of ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(84)90687-9
更新日期：1984-05-31 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1995.1309
更新日期：1995-03-08 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(83)91561-9
更新日期：1983-11-30 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2015.02.030
更新日期：2015-03-27 00:00:00
abstract：:Abnormal plasma ascorbic acid (AA) and dehydroascorbic acid (DHAA) levels observed in diabetes may be correlated to a deficiency in the recycling of AA. Ascorbic acid and DHAA levels are altered in diabetic liver in the present study. In addition, a coupling of the hexose monophosphate (HMP) shunt by way of NADPH to g...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1993.1365
更新日期：1993-03-31 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2019.04.140
更新日期：2019-06-18 00:00:00
abstract：:Regarding the molecular mechanism of dynamin in receptor-mediated endocytosis, GTPase activity of dynamin has been thought to have a critical role in endocytic vesicle internalization. However, a recent report suggested that GTP-binding to dynamin itself activates the dynamin to recruit molecular machinery necessary f...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.2001.4681
更新日期：2001-04-27 00:00:00
abstract：:Two adenylate cyclase inhibitors: 9-(tetrahydro-2-furyl)adenine and 2'5'-dideoxyadenosine decreased cAMP levels in LH-stimulated immature rat Leydig cells by 20-40%, independent of the concentration of LH. Steroid production was not correlated with this decrease in cAMP, but was increased (146%). The phorbol ester 4 b...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(85)91063-0
更新日期：1985-05-16 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.2000.4172
更新日期：2001-01-26 00:00:00
abstract：:In order to test the biological importance of amino acids in the C-terminal quarter of aminoglycoside 3'-phosphotransferase II enzyme, seven of the highly conserved residues in this region, His-188, Asp-190, Asp-208, Gly-210, Arg-211, Asp-216 and Asp-220, were changed via site-directed mutagenesis. The phenotype of ea...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(92)91715-3
更新日期：1992-06-30 00:00:00
abstract：:The effect of the mitochondrial dye rhodamine 123 (Rho 123) on protein synthesis (PS) activity was investigated in mitochondria isolated from liver and from both chloroma and erythroleukemia tumors. Incorporation of labelled leucine into mitochondrial protein was used to measure the rate of PS. While PS specific activ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(86)91144-7
更新日期：1986-06-13 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/s0006-291x(03)01172-0
更新日期：2003-08-01 00:00:00
abstract：:Heat shock protein 70 (HSP70) is an intracellular stress protein that confers cytoprotection to a variety of cellular stressors. Several lines of evidence have suggested that augmentation of the heat shock response by increasing the expression of HSP70 represents a potential therapeutic strategy for the treatment of c...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/s0006-291x(02)02986-8
更新日期：2003-01-31 00:00:00
abstract：:The refolding kinetics of cobrotoxin (CBTX), a small-molecular-weight ( approximately 7 kDa) all beta-sheet protein, has been monitored using a variety of biophysical techniques. The secondary structure formation and hydrophobic collapse occur as distinct events during the refolding of the protein. Complete secondary ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1999.0901
更新日期：1999-06-24 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2008.10.046
更新日期：2008-12-19 00:00:00
abstract：:The proto-oncogene Akt is a potent inhibitor of apoptosis, and it is activated in many human cancers. A number of recent studies have highlighted the importance of the inositol 1,4,5-trisphosphate (IP3) receptor(IP3R) in mediating calcium (Ca2+) transfer from the endoplasmic reticulum (ER) to the mitochondriain severa...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2008.07.153
更新日期：2008-10-31 00:00:00
abstract：:The soil fungus Mucor genevensis was shown to convert exogenous arachidonic acid to the oxylipin 3-hydroxy-5Z,8Z-tetradecadienoic acid (3-HTDE) as determined by gas chromatography/mass spectrometry. This metabolite was only found in the aqueous supernatant together with free linoleic acid, but not in the final fungal ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1998.9853
更新日期：1998-12-30 00:00:00
abstract：:Cultures of transformed human embryonic kidney 293 cells were transiently transfected with minigene constructs coding for the Abeta peptide (1-43). The Abeta minigene used in this study consisted of exons 16 and 17 of the amyloid precursor protein gene, including the 6000+ bp intronic region. Two of the constructs use...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1996.0469
更新日期：1996-03-27 00:00:00
abstract：:Molecular analysis of the induction of the lutropin/choriogonadotropin receptor during the process of luteinization of the rat ovary was performed. The appearance of receptor binding activity and an immunological analysis of the receptor using Triton solubilized membrane proteins show little receptor present in luteal...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(90)91177-t
更新日期：1990-09-14 00:00:00
abstract：:The rate limiting step in 5-fluorouracil catabolism is catalyzed by the enzyme dihydropyrimidine dehydrogenase. Since degradation of 5-fluorouracil decreases its efficacy in chemotherapy, the inhibition of its catabolism is a promising tool. We investigated the formation of micronuclei in vitro in mouse L5178Y cells. ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1994.2299
更新日期：1994-09-15 00:00:00
abstract：:The binding of leptin to hypothalamic neurons elicits inhibition of orexigenic NPY/AgRP neurons and stimulation of anorexigenic POMC/CART neurons. Projections of serotonergic neurons onto POMC neurons suggest that leptin and serotonin converge onto POMC neurons to regulate body weight. We probed the interaction of the...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2006.10.033
更新日期：2006-12-15 00:00:00
abstract：:Restenosis after angioplasty is thought to be caused by proliferation and migration of vascular smooth muscle cells (VSMCs), and it is a most serious problem in medical treatment. A low dose (50 ng/ml) of manumycin A, an inhibitor of p21(ras) (ras) farnesylation, significantly inhibited proliferation of rat VSMCs stim...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1999.1546
更新日期：1999-11-02 00:00:00
abstract：:Sex determining region Y-box 2 (SOX2) is well known as one of the "stemness" factors and is often expressed in cancers including breast cancer. In this study, we developed a reporter system using fluorescent protein driven by the promoter for SOX2 gene to detect and isolate living SOX2-positive cells. Using this syste...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2013.06.038
更新日期：2013-07-26 00:00:00
abstract：:The HSP30 gene of the budding yeast Saccharomyces cerevisiae encodes a seven-transmembrane heat shock protein expressed in response to various types of stress including heat shock. Although Hsp30p contains a potential N-glycosylation consensus sequence (Asn(2)-Asp(3)-Thr(4)), whether it is actually N-glycosylated has ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2012.02.126
更新日期：2012-03-30 00:00:00
