Abstract:
:We have used two different, but complementary assays to characterize functions of SV40 T antigen that are necessary for its ability to immortalize rat embryo fibroblasts. In accordance with previous work, we found that several functions were required. These include activities that map to the p53 binding domain and the amino terminal 176 amino acids which contain the J domain as well as the CR1 and CR2 domain required for binding and sequestering the RB family of pocket proteins. Moreover, we found that even though activities dependent only upon the amino terminus were sufficient for immortalization they were unable to maintain it. This suggests that immortalization by these amino terminal functions requires either additional events or immortalization of a subset of cells within the heterogeneous rat embryo fibroblast population. We further found that an activity dependent upon amino acids 17 - 27 which remove a portion of the CR1 domain and the predicted alpha-1 helix of the J domain was not necessary to maintain growth but was required for direct immortalization suggesting that at least one of the functions required initially was not required to maintain the immortal state. This represents the first demonstration that some of the functions required for maintenance of the immortal state differ from those required for initiation of immortalization.
journal_name
Oncogenejournal_title
Oncogeneauthors
Powell AJ,Darmon AJ,Gonos ES,Lam EW,Peden KW,Jat PSdoi
10.1038/sj.onc.1203154subject
Has Abstractpub_date
1999-12-02 00:00:00pages
7343-50issue
51eissn
0950-9232issn
1476-5594journal_volume
18pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Cytokine receptors have different signaling requirements which ultimately lead to various physiological responses. In an effort to precisely characterize the molecular determinants involved in the proliferative response mediated by cytokines, we examine dose-dependent proliferation of the betac (GM-CSF, IL-3, IL-5) an...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205444
更新日期:2002-05-16 00:00:00
abstract::Localized, nonindolent prostate cancer (PCa) is characterized by large-scale genomic rearrangements, aneuploidy, chromothripsis, and other forms of chromosomal instability (CIN), yet how this occurs remains unclear. A well-established mechanism of CIN is the overproduction of centrosomes, which promotes tumorigenesis ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0995-z
更新日期:2020-01-01 00:00:00
abstract::Nuclear-targeted high molecular weight 24 kDa fibroblast growth factor 2 (FGF-2) may induce specific cell functions through intracrine mechanisms. The role of nuclear FGF-2 on the metastatic potential of carcinoma cells was examined by conditional FGF-2 expression, which demonstrated that spontaneous metastasis in nud...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207638
更新日期:2004-06-10 00:00:00
abstract::Raf-1 is a serine-threonine protein kinase that functions as a central component of the mitogen-activated protein kinase signal transduction pathway. Raf-1 activity is currently assayed in vitro by either measuring 32P incorporation into MEK, Raf-1's only characterized substrate, or by using the phosphorylated MEK to ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203862
更新日期:2000-10-12 00:00:00
abstract::Invadopodia are cell protrusions that mediate cancer cell extravasation but the microenvironmental cues and signaling factors that induce invadopodia formation during extravasation remain unclear. Using intravital imaging and loss of function experiments, we determined invadopodia contain receptors involved in chemota...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0667-4
更新日期:2019-05-01 00:00:00
abstract::Aurora kinases are known to play a key role in maintaining mitotic fidelity, and overexpression of aurora kinases has been noted in various tumors. Overexpression of aurora kinase activity is thought to promote cancer development through a loss of centrosome or chromosome number integrity. Here we observed augmentatio...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208293
更新日期:2005-02-03 00:00:00
abstract::It has recently been shown that the high-risk human papillomavirus (HPV) E6 proteins can target the PDZ-domain containing proteins, Dlg, MUPP-1, MAGI-1 and hScrib for proteasome-mediated degradation. However, the E6 proteins from HPV-16 and HPV-18 (the two most common high-risk virus types) differ in their ability to ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204719
更新日期:2001-09-06 00:00:00
abstract::This study addresses the question of whether loss of p16INK4 expression contributes to the immortalization of human cells. In vitro immortalization usually proceeds through two phases. In the first phase (lifespan extension), cells continue proliferating and their telomeres continue shortening beyond the point at whic...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-09-19 00:00:00
abstract::Normal function of the p53 gene is integral to the cellular response to genotoxic stress. One prediction arising from this is that p53 deficiency results in an increased mutation frequency. However, limited evidence has been produced in support of this idea. In order to further investigate the in vivo role of p53 in s...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201040
更新日期:1997-05-01 00:00:00
abstract::Ferritin is the major intracellular iron storage protein that sequesters excess free iron to minimize generation of iron-catalysed reactive oxygen species. We previously demonstrated that expression of ferritin heavy chain (ferritin H) was induced by pro-oxidants, which is a part of cellular antioxidant response to pr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208901
更新日期:2005-11-17 00:00:00
abstract::The proangiogenic cytokine Interleukin-3 (IL-3) is released by inflammatory cells in breast and ovarian cancer tissue microenvironments and also acts as an autocrine factor for human breast and kidney tumor-derived endothelial cells (TECs). We have previously shown that IL-3-treated endothelial cells (ECs) release ext...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-017-0034-x
更新日期:2018-03-01 00:00:00
abstract::To investigate the tumorigenic potential of mutant p53 when selectively expressed in lung tissue, a transgenic mouse model was developed in which a mutant form of p53 (p53-273H) was placed under the transcriptional control of the lung-specific human surfactant protein C (SP-C) promoter. Two founder mice were identifie...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205909
更新日期:2002-11-07 00:00:00
abstract::The SCL/TAL1 gene was originally identified by virtue of its rearrangement and transcriptional activation in patients with T cell acute lymphoblastic leukaemia. It encodes a helix-loop-helix transcription factor, is not normally expressed in T cells, but is expressed in erythroid, mast, megakaryocytic and progenitor c...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-07-06 00:00:00
abstract::Loss of heterozygosity on chromosome 10p was observed frequently in human prostate cancers. Studies have demonstrated that the introduction of the short arm of human chromosome 10 into a human prostate cancer cell line, PPC-1, by microcell-mediated chromosome transfer (MMCT), suppressed the malignant phenotype, sugges...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204079
更新日期:2001-01-18 00:00:00
abstract::Cancer development results from deregulated control of stem cell populations and alterations in their surrounding environment. Notch signaling is an important form of direct cell-cell communication involved in cell fate determination, stem cell potential and lineage commitment. The biological function of this pathway ...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2008.225
更新日期:2008-09-01 00:00:00
abstract::TSC1 and TSC2 are responsible for the tumor suppressor gene syndrome tuberous sclerosis (TSC). Mammalian TSC genes have been shown to be involved in cell cycle regulation. Recently, in Drosophila, these data have been confirmed and TSC genes have further been demonstrated to affect cell size control. Here we provide s...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206776
更新日期:2003-07-31 00:00:00
abstract::A limited number of adult stem cells (SCs) maintain the homoestasis of different tissues through the lifetime of the individual by generating differentiating daughters and renewing themselves. Errors in the SC division rate or in the fine balance between self-renewal and differentiation might result in tissue overgrow...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2008.349
更新日期:2008-11-24 00:00:00
abstract::By NIH3T3 transfection assay in conjunction with in vitro transient neomycin selection, activated c-H-ras-1 oncogenes were detected in two squamous cell carcinoma cell lines, ZA and HOC-313, newly established from human oral cancer patients. ZA had a point mutational activation at the 13th codon, this activation of c-...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::PML is a component of a multiprotein complex, termed nuclear bodies, and the PML protein was originally discovered in patients suffering from acute promyelocytic leukaemia (APL). APL is associated with a reciprocal chromosomal translocation of chromosomes 15 and 17, which results in a fusion protein comprising PML and...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1204765
更新日期:2001-10-29 00:00:00
abstract::Minichromosome maintenance (MCM) proteins 2-7 are important in DNA replication licensing. Functional roles beyond licensing are speculated. In addition, significances in medulloblastoma (MB) remain unclear. In this study, we showed the frequent deregulation of MCM2 and MCM3 expression in 7 MB cell lines and 31 clinica...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.287
更新日期:2010-10-07 00:00:00
abstract::Loss of Fanconi anemia (FA) proteins activity by recessive inherited mutations in one of the FA genes leads to a disease characterized by bone marrow failure, myeloid leukemia and DNA damage hypersensitivity. The aim of this work was to improve our understanding of the FA syndrome defining the transcription profile of...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207677
更新日期:2004-06-24 00:00:00
abstract::Oncoprotein C-MYC is overexpressed in human metastatic melanomas and melanoma-derived cells where it is required for the suppression of oncogene-induced senescence (OIS). The genetic events that maintain high levels of C-MYC in melanoma cells and their role in OIS are unknown. Here we report that C-MYC in cells from s...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.339
更新日期:2012-03-22 00:00:00
abstract::We have isolated mouse cDNA clones encoding Tyro 3, a receptor protein-tyrosine kinase (PTK) of the mammalin central nervous system (CNS). Expression of the Tyro 3 gene is strongly up-regulated in neurons of the mouse neocortex, cerebellum, and hippocampus after the day of birth, during periods of active synaptogenesi...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Rad23 proteins function in both DNA repair and protein stability regulation. As ubiquitinated forms of p53 are stabilized after DNA damage in concert with p53 functional activation, and human Rad23 proteins (hHR23A and B) regulate p53 stability in unstressed cells, the role of hHR23B in post-genotoxin regulation of p5...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209865
更新日期:2007-02-22 00:00:00
abstract::The heterogeneous breast cancers can be classified into different subtypes according to their histopathological characteristics and molecular signatures. Foxa1 expression is linked with luminal breast cancer (LBC) with good prognosis, whereas Twist1 expression is associated with basal-like breast cancer (BLBC) with po...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.286
更新日期:2017-02-23 00:00:00
abstract::Members of the Myc proto-oncogene family encode transcription factors that function in multiple aspects of cell behavior, including proliferation, differentiation, transformation and apoptosis. Recent studies have shown that MYC activities are modulated by a network of nuclear bHLH-Zip proteins. The MAX protein is at ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201611
更新日期:1998-02-26 00:00:00
abstract::Emerging evidence has shown that cancer stem cells (CSCs) are the cellular determinants to promote cancer invasion and metastasis. However, the mechanism underlying CSC invasion remains unknown. MicroRNAs are evolutionally conserved small noncoding RNAs that are critical for the regulation of gene expression, and thei...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.75
更新日期:2015-03-12 00:00:00
abstract::Mouse plasmacytomas induced by pristane oil alone, or in combination with Abelson murine leukemia virus (A-MuLV), regularly carry one of three alternative chromosomal translocations that juxtapose c-myc to immunoglobulin heavy- or light-chain loci. E mu-c-myc transgenic mice develop translocation-free plasmacytomas af...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Lysine methylation of histones and non-histone substrates by the SET domain containing protein lysine methyltransferase (KMT) G9a/EHMT2 governs transcription contributing to apoptosis, aberrant cell growth, and pluripotency. The positioning of chromosomes within the nuclear three-dimensional space involves interaction...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0723-8
更新日期:2019-05-01 00:00:00
abstract::Elucidating the mechanisms involved in sensitizing radioresistant tumors to ionizing radiation (IR) treatments while minimizing injury to surrounding normal tissue is an important clinical goal. Due to their sequence-derived specificity and properties as gene regulators in IR-affected pathways, microRNAs (miRNAs) coul...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.222
更新日期:2017-01-26 00:00:00