Abstract：
:The suitability of CK20 mRNA expression as a marker for the detection of minimum residual disease in patients with cancer of epithelial origin was evaluated. A sensitive nested RT-PCR assay with multiple replicates was optimised to detect a minimum number of circulating tumor cells expressing cytokeratin 20 (CK20) mRNA. Using this optimal procedure, we examined CK20 mRNA expression in ten epithelial and seven leukemic cell lines, in eight bladder tumors, in peripheral blood samples from 18 tumor patients and from 29 healthy controls and in 8 bone marrow samples from healthy donors. CK20 mRNA was found in 13 of 18 (72%) blood samples from patients with cancer of epithelial origin and in all the epithelial tumor cells tested. However, CK20 mRNA was also detected in 21 of 29 (72%) bloods, in 8 of 8 bone marrow samples from healthy donors and in 4 of 7 leukemic cell lines. These results highlight a requirement for either determination of threshold levels of CK20 normal expression or the development of quantitative techniques to distinguish between a tumor-specific CK20 gene expression and a low level background transcription of this marker. These results would also advise caution in using CK20 as a tumor specific marker in clinical investigations.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Champelovier P,Mongelard F,Seigneurin Dsubject
Has Abstractpub_date
1999-05-01 00:00:00pages
2073-8issue
3Aeissn
0250-7005issn
1791-7530journal_volume
19pub_type
杂志文章abstract：BACKGROUND:Metastatic involvement of the pancrease is not an uncommon autopsy finding, however, pancreatic metastasis is an infrequent clinical condition and the pattern of the metastases have not been studied. We conducted a retrospective study in order to evaluate the pattern of the metastasis from lung cancer. PATI...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Caspase-3 is a key executioner cysteine protease involved in programmed cell death or apoptosis. A ribozyme to human caspase-3 was designed, tested by in vitro cleavage, and transfected into a drug-resistant variant (DLKP-A5F) of a human lung carcinoma cell line (DLKP). By both stable and transient transfection, this ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:Concomitant neoplasm and abdominal aortic aneurysm (AAA) is not a rare clinical association and can pose problems if antiblastic treatment is required. The literature shows lack of consensus owing to the fact that AAA can modify the liquid load and the hemodynamic setting so that chemotherapy toxicity profile becomes ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:It has been suggested that prognosis in familial breast cancer could be influenced by the parent of origin, with a worse prognosis when inherited paternally. This study aimed to investigate the effect of the parent of origin on prognosis. PATIENTS AND METHODS:Index patients were divided into two study group...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11442
更新日期：2017-03-01 00:00:00
abstract：:A circadian rhythm of serum concentrations of two tumor markers (CEA and AFP) was demonstrated in individuals not suffering from cancer. These rhythms exhibited a diurnal peak with a nocturnal dip. They disappeared in cancer groups. However, in some cancer subjects, a circadian activity was still detectable, but with ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：BACKGROUND:3-Chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), a disinfection by-product in chlorinated drinking water, induces follicular tumors in thyroid glands in Wistar rats. The mechanisms of the MX-induced thyroid gland tumorigenesis are not known. MATERIALS AND METHODS:The expressions of p53 (primary an...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:Dihydropyrimidine dehydrogenase (DPD) is the rate-limiting enzyme of 5-fluorouracil (5-FU) catabolism. Several studies have demonstrated the clinical importance of DPD in cancer patients, suggesting that the efficacy of 5-FU may be related to DPD activity in tumor tissue. In the present study, DPD activity a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:A better understanding of the non-targeted (bystander) effects of radiation may have important implications with regards to radiation risk assessment, radiation protection, and targeted cancer therapy. In the present study, the direct and bystander effects of α-particle irradiation in immortalized human fibroblasts (F...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:Most patients with pancreatic adenocarcinoma are diagnosed with locally advanced (unresectable) or metastatic disease. The aim of this study was to investigate possible prognostic factors of survival in such patients. PATIENTS AND METHODS:Two hundred and fifteen patients were studied retrospectively. Twenty...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:To identify retinoic acid (RA) signalling pathways involved in growth and differentiation in cells of the glial lineage, two human glioma ceh lines were studied. The three RA receptors (RARs) mRNAs were constitutively expressed, and of the three RXRs, RXR beta appeared predominant. Western blotting analysis confirmed ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Grape seed extracts were more cytotoxic than grape peel extracts. Methanol and 70% methanol extracts of grape seed selectively killed two human oral tumor cell lines, more efficiently than human gingival fibroblasts. ESR spectroscopy revealed that these extracts produced radicals under alkaline conditions and enhanced...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The aim of this study was to compare the accuracy of 18-fluoro-2-deoxyglucose (FDG) positron emission tomography (PET) and thallium-201 (Tl-201) single photon emission computed tomography (SPECT) in differentiating recurrent/residual nasopharyngeal carcinomas (NPC) from post-radiotherapy (RT) changes in patients with ...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:Four heart hormones, namely atrial natriuretic peptide (ANP), long-acting natriuretic peptide (LANP), vessel dilator and kaliuretic peptide reduce up to 97% of cancer cells in vitro. These four cardiac hormones eliminate up to 80% of human pancreatic adenocarcinomas, two-thirds of human breast carcinomas and up to 86%...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.11152
更新日期：2016-11-01 00:00:00
abstract：:Megakaryocyte polyploidization responds to platelet demand and results from the lack of cytoplasmic separation while the nucleus keeps dividing. In normal telophase, the plane of the actin constriction ring is determined by the tubulin spindle. In order to investigate the role of tubulin in the megakaryocyte polyploid...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：BACKGROUND/AIM:Tyrosine kinase inhibitors are important in the treatment of metastatic renal cell cancer (mRCC). The aim of the study was to evaluate the costs and effects of sunitinib in mRCC. PATIENTS AND METHODS:A total of 81 mRCC patients who received first-line sunitinib therapy between 2010 and 2014 were recruit...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13749
更新日期：2019-10-01 00:00:00
abstract：:The cell proliferation activity of sixteen childhood primitive neuroectodermal tumors (PNETs) was observed immunocytochemically, to determine the cell kinetics and cell proliferation activity of these relatively undifferentiated, malignant brain tumors. Two mouse anti-human monoclonal antibodies (MoABs) were employed ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:A human metastatic meningiosarcoma has been established as a cell line as follows: At autopsy performed 12 hours after death, tumour nodules from the left parietal pleura were obtained under sterile conditions, minced and inoculated into culture vessels. The cells obtained were cloned, and clones with abnormal chromos...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:In colorectal cancer, the predictive value of the currently used staging method is limited. Therefore, many parameters have been studied to improve the prediction of final clinical outcome, including several aspects of the primary tumor that are associated with its aggressiveness and the capacity of the host response....
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：BACKGROUND/AIM:This study aimed to investigate the bolus practice among Italian radiation oncologists. PATIENTS AND METHODS:In 2018, a survey on bolus application was sent to all members of the Italian Association of Radiotherapy and Clinical Oncology. RESULTS:The survey was joined by 102 radiation oncologists. Not a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14673
更新日期：2020-11-01 00:00:00
abstract：BACKGROUND:Primary small cell carcinoma of the ovary (SCCO) is rare, making prognosis and outcomes largely undefined. PATIENTS AND METHODS:Using case listing session of SEER 18 (1973-2010), we examined outcomes for patients with SCCO. Analyses were conducted with SEER(*)Stat 8.1.2, Microsoft Excel 2007 and GraphPad Pr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
abstract：BACKGROUND/AIM:AZD8055 is an inhibitor of mammalian target of rapamycin (mTOR) that can suppress both mTOR complex 1 (mTORC1) and mTORC2. This study investigated the antitumor effects of AZD8055 on colon cancer. MATERIALS AND METHODS:The effects of AZD8055 on proliferation, apoptosis, and cell cycle of colon cancer ce...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12369
更新日期：2018-03-01 00:00:00
abstract：:Mutations occurring in sporadic epithelial ovarian carcinomas are reviewed and their functional significance in terms of prognosis and prediction of anticancer drug activity are discussed. Alterations in the BRCA1/2 genes, TP53, PTEN, PI3Kinase, KRAS/BRAF and CTNNB1 are described. TP53 is likely to be a driver in high...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2011-08-01 00:00:00
abstract：:The influence of several oncogenes on drug resistance has recently been show by transfection studies. Therefore, we investigated the influence of transfection on adriamycin (ADR) in cell line transfected with several oncogenes accompanied with pSV2neo vector. There were differences in both the intracellular accumulati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:At present the available treatment modalities are not therapeutic for disseminated stage III- and IV melanoma. The management of these patients therefore concentrates primarily on effective palliation taking care to improve the quality of life. As an exception from this rule, patients with regional or solitary metasta...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：BACKGROUND:The aim of this study was to evaluate whether human papilloma virus (HPV) testing at LLETZ for CIN can be useful to identify patients with high risk of recurrent disease. MATERIALS AND METHODS:In 62 women treated with LLETZ for CIN the status of the resection margins was recorded and related to the HPV stat...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND/AIM:Roles for mutant (mt) KRAS in the innate immune microenvironment in colorectal cancer (CRC) were explored. MATERIALS AND METHODS:Human CRC HCT116-derived, mtKRAS-disrupted (HKe3) cells that express exogenous mtKRAS and allogenic cytokine-activated killer (CAK) cells were co-cultured in 3D floating (3DF)...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14465
更新日期：2020-08-01 00:00:00
abstract：:The incidence of breast cancer is increasing rapidly in Taiwan but is still low compared with western countries. The impact of body size factors in western countries inspired us to have a case-control study. A matched case-control study was done on 122 pairs of incident cases of female breast cancer and community cont...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:Because of its poor prognosis, new modalities to treat pancreatic cancer are highly welcome. Gammalinolenate (GLA) has been shown to possess antitumor activity on various human cancer cell lines in vitro and some evidence has been found of its modulative activity on tubulin active agents, such as vinca alkaloids. GLA ...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：BACKGROUND:Prediction of biological behavior is crucial for selection of new therapeutic modalities in GIST. Here, we aimed to assess whether KIT and PDGFRA mutations have survival impact in gastrointestinal stromal tumors (GIST). PATIENTS AND METHODS:Fifty-five Brazilian patients with completely resected GIST were ex...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND:Several studies have demonstrated the efficacy of systemic oxaliplatin (Oxa) in combination with 5-fluorouracil (5-FU) and folinic acid (FA) for the treatment of colorectal liver metastases (CRLM). However, nothing is presently known about the pharmacokinetics of Oxa administered via the hepatic artery and o...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2003-11-01 00:00:00
