An easy and safe method to store and disinfect explanted skull bone.

Abstract:

:In our department extensive decompression craniectomies became the treatment of choice for patients with massive cerebral oedema following either trauma or acute cerebral infarction. The remarkable survival rates of this neurosurgical technique created the problem of adequate vault defect reconstruction. To evaluate the biological safety of using stored autologous skull flaps for this purpose, we compared three different disinfection methods. Skull bone fragments stored at -21 degrees C for different periods of time were artificially contaminated with clinically relevant strains of Serratia marcescens, Enterococcus faecium and Staphylococcus aureus. As potential methods for disinfection we tested immersion in 3% H2O2, boiling in normal saline for 15 and 30 minutes and a special process of steam disinfection at a temperature of 75 degrees C for 20 minutes. We were able to demonstrate that only steam disinfection completely eliminated the bacterial strains tested. Refrigeration plus steam disinfection of autologous skull bone prior to re-implantation seems to offer reliable safety for its use for defect closure. It is available at reasonable cost in many hospitals and does not require a bone bank.

journal_name

Acta Neurochir (Wien)

journal_title

Acta neurochirurgica

authors

Schültke E,Hampl JA,Jatzwauk L,Krex D,Schackert G

doi

10.1007/s007010050335

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

525-8

issue

5

eissn

0001-6268

issn

0942-0940

journal_volume

141

pub_type

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