Abstract:
:Regulatory genes directing embryonic development are expressed in complex patterns. The Drosophila homeobox gene fushi tarazu (ftz) is expressed in a striped pattern that is controlled by several discrete and large cis- regulatory elements. One key cis-element is the ftz proximal enhancer which is required for stripe establishment and which mediates autoregulation by direct binding of Ftz protein. To identify the trans-acting factors that regulate ftz expression and autoregulation, we developed a modified yeast two hybrid screen, the Double Interaction Screen (DIS). The DIS was designed to isolate both DNA binding transcriptional regulators that interact with the proximal enhancer and proteins that interact with Ftz itself when it is bound to the enhancer. The screen identified two candidate Ftz protein cofactors as well as activators and repressors of ftz transcription that bind directly to the enhancer. One of these (Tramtrack (Ttk)) was previously shown to bind to at least five sites in the proximal enhancer; genetic studies suggested that Ttk acts as a repressor of ftz in the embryo. Here we show that, in yeast cells, Ttk protein strongly activates transcription, suggesting that yeast may be missing a necessary co-repressor which is present in Drosophila embryos. Further, we have characterized the activity of a second candidate ftz repressor isolated in the screen - the product of the pair-rule gene sloppy paired - a member of the forkhead family. We show that Slp1 is a DNA binding protein. We have identified a high affinity binding site for Slp1 in the ftz proximal enhancer. Slp1 represses transcription via this binding site in yeast cells, consistent with its role as a direct repressor of ftz stripes in interstripe regions during late stages of embryogenesis. The DIS should be a generally useful method to identify DNA binding transcriptional regulators and protein partners of previously characterized DNA binding proteins.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Yu Y,Yussa M,Song J,Hirsch J,Pick Ldoi
10.1016/s0925-4773(99)00038-6subject
Has Abstractpub_date
1999-05-01 00:00:00pages
95-105issue
1-2eissn
0925-4773issn
1872-6356pii
S0925477399000386journal_volume
83pub_type
杂志文章abstract::The Drosophila fat facets and canoe genes regulate non-neural cell fate decisions during ommatidium formation. We have shown previously that the FAM (fat facets in mouse) de-ubiquitinating enzyme regulates the function of AF-6, (mammalian canoe homologue), in the MDCK epithelial cell line (Taya et al., 1998. The Ras t...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00294-4
更新日期:2000-03-01 00:00:00
abstract::Using in situ hybridization on whole-mounts and sections of mouse embryos we have visualized the pattern of expression for the Eph receptor ligand ephrin-A5. Non neuronal expression domains include the ectoderm of the branchial arches, the ectoderm and mesenchyme surrounding the dorsal root ganglia, the intervertebral...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00252-5
更新日期:2000-05-01 00:00:00
abstract::We have isolated and characterized two complete cDNA clones, Zfz8a and Zfz8b, which encode zebrafish Frizzled (Fz) homologues. The predicted protein sequences, spanning 579 and 576 amino acid residues for ZFz8a and ZFz8b, respectively, were highly homologous (78%) to each other and contained an extracellular cysteine-...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00137-3
更新日期:1998-11-01 00:00:00
abstract::Cloning and sequencing of mouse Mf2 (mesoderm/mesenchyme forkhead 2) cDNAs revealed an open reading frame encoding a putative protein of 492 amino acids which, after in vitro translation, binds to a DNA consensus sequence. Mf2 is expressed at high levels in the ventral region of newly formed somites, in sclerotomal de...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(97)00157-3
更新日期:1998-01-01 00:00:00
abstract::The neural crest (NC) is one of the most fascinating structures during embryonic development. Unique to vertebrate embryos, these cells give rise to important components of the craniofacial skeleton, such as the jaws and skull, as well as melanocytes and ganglia of the peripheral nervous system. Worldwide, several gro...
journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2018.07.009
更新日期:2018-10-01 00:00:00
abstract::The gene tprd, which contains three tetratricopeptide domains, has been recently localized in the Down syndrome (DS) chromosomal region 1. We have cloned a cDNA encoding part of the murine ortholog of tprd and used it to characterize the expression pattern of this gene during development and at the adult stage. At E8....
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00076-3
更新日期:1999-06-01 00:00:00
abstract::In sea urchin development, micromere descendants play important roles in skeletogenesis and induction of gastrulation. We previously reported that the T-brain homolog of sea urchin Hemicentrotus pulcherrimus, HpTb expresses specifically in micromere descendants and is required for induction of gastrulation and skeleto...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2007.10.009
更新日期:2008-01-01 00:00:00
abstract::We describe the sequence and expression pattern of Sp5, a novel member of the vertebrate Sp1 transcription factor gene family which consists of at least five members. This gene family is characterized by a highly conserved domain which is formed by three Zn fingers, which bind to the GC box or the GT/CACC box in the p...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00544-x
更新日期:2001-03-01 00:00:00
abstract::During the development of the proventriculus (glandular stomach) of the chicken embryo, the endodermal epithelium invades into the surrounding mesenchyme and forms glands. The glandular epithelial cells produce pepsinogen, while the non-glandular (luminal) epithelial cells secrete mucus. Sonic hedgehog is expressed un...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(03)00069-8
更新日期:2003-07-01 00:00:00
abstract::The selective sensitivity of cells to programmed cell death (PCD) depends on the positive and negative death-inducing signals that converge into the apoptotic pathway. In Drosophila, the midline glial (MG) cells undergo selective death during development. Here, we show that the long isoform of the RNA-binding protein ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.10.004
更新日期:2009-01-01 00:00:00
abstract::The segmentation of vertebrate embryos depends on a complex genetic network that generates highly dynamic gene expression. Many of the elements of the network have been identified, but their interaction and their influence on segmentation remain poorly understood. A few mathematical models have been proposed to explai...
journal_title:Mechanisms of development
pub_type: 杂志文章,评审
doi:10.1016/j.mod.2007.06.004
更新日期:2007-08-01 00:00:00
abstract::Active repression of gene expression mediated by unliganded nuclear receptors plays crucial roles in early development of vertebrates. N-CoR (nuclear receptor co-repressor) is the first identified co-repressor that can repress retinoic acid (RA) inducible gene transcription in the absence of RA. Previously, N-CoR was ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2009.09.001
更新日期:2009-10-01 00:00:00
abstract::The spatial and temporal expression of seven Drosophila protein tyrosine phosphatase genes during oogenesis was examined by whole mount in-situ hybridization of antisense RNA probes to ovaries. Our observations indicate diverse expression patterns consistent with multiple roles for protein tyrosine phosphatases in the...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(95)00432-z
更新日期:1995-10-01 00:00:00
abstract::Proper metazoan mesoderm development requires the function of a basic helix-loop-helix (bHLH) transcription factor, Twist. Twist-containing dimers regulate the expression of target genes by binding to E box promoter elements containing the site CANNTG. In Caenorhabditis elegans, CeTwist functions in a subset of mesode...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2007.01.005
更新日期:2007-05-01 00:00:00
abstract::Following tissue damage the immune response, including inflammation, has been considered an inevitable condition to build the host defense against invading pathogens. The recruitment of innate immune leukocytes to injured tissue is observed in both vertebrates and invertebrates. However, it is still not conclusive whe...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2012.11.005
更新日期:2013-02-01 00:00:00
abstract::The effects of signal perturbation on expression domains of molecular markers for the mesoderm and ectoderm have been analysed across the dorso-ventral axis in zebrafish embryos. Injection of RNA encoding bone morphogenetic protein-4 (BMP-4) ventralised the embryo, expanding the intermediate mesoderm and non-neural ec...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(97)00659-x
更新日期:1997-03-01 00:00:00
abstract::Xenopus embryos in solutions containing suramin show a dose-dependent decrease in the formation of dorsoanterior structures. Continuous treatment with 1 mM suramin produces embryos without mesodermal derivatives but with mesenchymal cells. Brief immersions of 20 min were used to determine the most sensitive stages and...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(94)90054-x
更新日期:1994-01-01 00:00:00
abstract::A new family of homeodomain proteins has recently been identified that includes extradenticle, ceh-20 and three mammalian proteins Pbx1, Pbx2 and Pbx3. We show here that two members of this family, Pbx1 and Pbx2 bind cooperatively to DNA with both Hoxb-7 and Hoxb-8. Engrailed-2 modulates the DNA binding activity of th...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(95)00394-g
更新日期:1995-07-01 00:00:00
abstract::The SRY-related, HMG box SoxB1 transcription factors are highly homologous, evolutionarily conserved proteins that are expressed in neuroepithelial cells throughout neural development. SoxB1 genes are down-regulated as cells exit the cell-cycle to differentiate and are considered functionally redundant in maintaining ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2008.10.005
更新日期:2009-01-01 00:00:00
abstract::In the developing eye of Drosophila the protein kinase D-Raf controls the specification of the R7 photoreceptor cells. We show that overexpression of wild-type D-Raf inhibits the formation of R7 cells in a dose-dependent manner. Conversely, overexpression of mutant D-Raf proteins in which the conserved S388 is replace...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(97)00052-x
更新日期:1997-06-01 00:00:00
abstract::During vertebrate eye development, the expression of the homeobox gene Six6 is restricted to the neural retina and is initiated later than Rx and Pax6 in the presumptive retina field. We show here that overexpression of mouse Six6 in Xenopus embryos can induce transformation of competent tissue of the anterior neural ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00271-9
更新日期:2000-05-01 00:00:00
abstract::NK-2 class homeobox genes are candidate patterning and lineage regulators in diverse organisms. We report here the embryonic expression pattern of murine member, Nkx2-6. In keeping with its vertebrate relatives, Nkx2-6 was transcribed in ventrolateral embryonic structures. Expression was first detected at E8.0 in endo...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00037-9
更新日期:1998-04-01 00:00:00
abstract::Hox gene expression imparts segment identity to body structures along the anterior-posterior axis and is tightly governed by higher order chromatin mechanisms. Chromatin regulatory features of the homeotic complex are best defined in Drosophila melanogaster, where multiple cis-regulatory elements have been identified ...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2015.07.015
更新日期:2015-11-01 00:00:00
abstract::Dickkopf-1 (dkk-1) is member of a novel family of secreted proteins and functions in head induction during Xenopus embryogenesis, acting as a potent inhibitor of Wnt signalling. Here we report: (1) the isolation of two additional murine members of the dkk family, dkk-2 and dkk-3; and (2) analysis of adult and embryoni...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(99)00138-0
更新日期:1999-09-01 00:00:00
abstract::The embryonic fins and the tip of adult fins of teleost fish are supported by rows of straight, unmineralized fibrils called actinotrichia. The proximal ends of the actinotrichia are bundled and the mineralized bones called lepidotrichia are made along them. Since malformation in actinotrichia causes wavy fin bones, t...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2018.08.010
更新日期:2018-10-01 00:00:00
abstract::The idea has been put forward that molecules and mechanisms acting during development are re-used during regeneration in the adult, for example in response to traumatic injury in order to re-establish the functional integrity of neuronal circuits. Members of the Eph family of receptor tyrosine kinases and their 'ligan...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00431-2
更新日期:2001-08-01 00:00:00
abstract::We have identified a short segment of the mouse Pax-6 gene 5' flanking region that is necessary and sufficient for reporter construct expression in components of the eye derived from non-neural ectoderm. This transcriptional control element has a highly conserved nucleotide sequence over 341 bp and is located approxim...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00057-4
更新日期:1998-05-01 00:00:00
abstract::Using the epidermis-specific cytokeratin 14 promoter to deliver HGF exclusively from epidermal keratinocytes, we have examined the potential of hepatocyte growth factor (HGF) secreted from the normal environment to control morphogenesis. The transgenic mice displayed a significant increase of the number of melanocytes...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00308-7
更新日期:2000-06-01 00:00:00
abstract::In the Drosophila embryo, a subset of muscles require expression and function of the RYK subfamily RTK gene derailed (drl) for correct attachment. We have isolated a second RYK homolog, doughnut (dnt), from Drosophila. The DNT protein exhibits 60% amino acid identity to DRL, and is structurally as similar to the mamma...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00167-1
更新日期:1998-11-01 00:00:00
abstract::Ovotestis development in B6-XY(POS) mice provides a rare opportunity to study the interaction of the testis- and ovary-determining pathways in the same tissue. We studied expression of several markers of mouse fetal testis (SRY, SOX9) or ovary (FOXL2, Rspo1) development in B6-XY(POS) ovotestes by immunofluorescence, u...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2009.02.006
更新日期:2009-05-01 00:00:00