Abstract:
:Islet duodenal homeobox 1 (IDX-1/PF-1/STF-1/PDX-1), a homeodomain protein that transactivates the insulin promoter, has been shown by targeted gene ablation to be required for pancreatic development. After 90% pancreatectomy (Px), the adult pancreas regenerates in a process recapitulating embryonic development, starting with a burst of proliferation in the epithelium of the common pancreatic duct. In this model, IDX-1 mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction in total RNA from isolated common pancreatic ducts at levels 10% of those of isolated islets. The IDX-1 mRNA levels were not significantly different for common pancreatic ducts of Px, sham Px, and unoperated rats and did not change with time after surgery. By immunoblot analysis, IDX-1 protein was only faintly detected in these ducts 1 and 7 days after Px or sham Px but was easily detected at 2 and 3 days after Px. Similarly, IDX-1 immunostaining was barely detectable in sham or unoperated ducts but was strong in ducts at 2-3 days after Px. The increase of IDX-1 immunostaining followed that of BrdU incorporation (proliferation). These results indicate a posttranscriptional regulation of the IDX-1 expression in ducts. In addition, islets isolated 3-7 d after Px showed higher IDX-1 protein expression than control islets. Thus, in pancreatic regeneration IDX-1 is upregulated in newly divided ductal cells as well as in islets. The timing of enhanced expression of IDX-1 implies that IDX-1 is not important in the initiation of regeneration but may be involved in the differentiation of ductal cells to beta-cells.
journal_name
Diabetesjournal_title
Diabetesauthors
Sharma A,Zangen DH,Reitz P,Taneja M,Lissauer ME,Miller CP,Weir GC,Habener JF,Bonner-Weir Sdoi
10.2337/diabetes.48.3.507subject
Has Abstractpub_date
1999-03-01 00:00:00pages
507-13issue
3eissn
0012-1797issn
1939-327Xjournal_volume
48pub_type
杂志文章相关文献
DIABETES文献大全abstract:OBJECTIVE:Leukocyte infiltration of adipose is a critical determinant of obesity-related metabolic diseases. Fractalkine (CX3CL1) and its receptor (CX3CR1) comprise a chemokine system involved in leukocyte recruitment and adhesion in atherosclerosis, but its role in adipose inflammation and type 2 diabetes is unknown. ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-0956
更新日期:2011-05-01 00:00:00
abstract::Although decreased levels of circulating insulinlike growth factor I (IGF-I) and hepatic IGF-I mRNA in diabetic animal models support a role for insulin in IGF-I regulation, it has been difficult to study the effects of insulin in vitro. Few immortal cell lines exhibit hormone-responsive production of IGF-I, and studi...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.40.11.1525
更新日期:1991-11-01 00:00:00
abstract::PTPN22 encodes the lymphoid tyrosine phosphatase (LYP) and is the second strongest non-HLA genetic risk factor for type 1 diabetes. The PTPN22 susceptibility allele generates an LYP variant with an arginine-to-tryptophan substitution at position 620 (R620W) that has been reported by several studies to impart a gain of...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-0929
更新日期:2013-03-01 00:00:00
abstract::Earlier investigations have revealed that polyethylene glycol-B1-insulin (PEG insulin) causes lower utilization of glucose in fat cells than does the unaltered hormone, even though the blood glucose-lowering activity of both preparations in intact animals is identical. The present study was aimed to establish whether ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.10.953
更新日期:1983-10-01 00:00:00
abstract::The TaqIB cholesteryl ester transfer protein (CETP) gene polymorphism (B1B2) is a determinant of HDL cholesterol in nondiabetic populations. Remarkably, this gene effect appears to be modified by environmental factors. We evaluated the effect of this polymorphism on HDL cholesterol levels and on the lipoprotein respon...
journal_title:Diabetes
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.2337/diab.46.12.2082
更新日期:1997-12-01 00:00:00
abstract::Treatment of male sand rats kept on a balanced laboratory chow diet ad libitum with beta,beta'-tetramethyl-substituted hexadecanedioic acid (MEDICA 16) resulted in a hypolipidemic effect accompanied by an extensive reduction in adiposity, with a concomitant hypoglycemic-hypoinsulinemic effect. The overall effect was s...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.12.1618
更新日期:1988-12-01 00:00:00
abstract::Plasma dopamine-beta-hydroxylase (pDBH) activity is markedly elevated in chronic experimental streptozotocin (STZ) diabetes in the rat. Several possible explanations, all potentially relevant to the pathophysiology of diabetes, could explain this observation. The objective of this paper was to further delineate the be...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.30.5.416
更新日期:1981-05-01 00:00:00
abstract:OBJECTIVE:Given the pleiotropic effect of eicosapentaenoic acid (EPA), it is interesting to know whether EPA is capable of improving obesity. Here we examined the anti-obesity effect of EPA in mice with two distinct models of obesity. RESEARCH DESIGN AND METHODS:Male C57BL/6J mice were fed a high-fat/high-sucrose diet...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db09-1554
更新日期:2010-10-01 00:00:00
abstract::Persistent nutrient excess results in a compensatory increase in the β-cell number in mammals. It is unknown whether this response occurs in nonmammalian vertebrates, including zebrafish, a model for genetics and chemical genetics. We investigated the response of zebrafish β-cells to nutrient excess and the underlying...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-1841
更新日期:2012-10-01 00:00:00
abstract::More than 120 published reports have described associations between single nucleotide polymorphisms (SNPs) and type 2 diabetes. However, multiple studies of the same variant have often been discordant. From a literature search, we identified previously reported type 2 diabetes-associated SNPs. We initially genotyped 1...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0461
更新日期:2007-01-01 00:00:00
abstract::Advanced glycoxidation end products (AGEs) are implicated in delayed diabetic wound healing. To test the role of diet-derived AGE on the rate of wound healing, we placed female db/db (+/+) (n = 55, 12 weeks old) and age-matched control db/db (+/-) mice (n = 45) on two diets that differed only in AGE content (high [H-A...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.11.2805
更新日期:2003-11-01 00:00:00
abstract::Sirt6 is an NAD+-dependent deacetylase that is involved in the control of energy metabolism. However, the tissue-specific function of Sirt6 in the adipose tissue remains unknown. In this study, we showed that fat-specific Sirt6 knockout (FKO) sensitized mice to high-fat diet-induced obesity, which was attributed to ad...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-1225
更新日期:2017-05-01 00:00:00
abstract::Studies have been carried out on activities of lysosomal beta-N-acetylhexosaminidase (hex), beta-galactosidase (beta-gal), alpha-glucosidase (alpha-glu), and acid phosphatase (AP) in serum and urine from patients with juvenile diabetes and matched controls. There is a large increase in blood and urinary hex activity (...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.25.5.420
更新日期:1976-05-01 00:00:00
abstract::Vasculopathy is a major complication of diabetes; however, molecular mechanisms mediating the development of vasculopathy and potential strategies for prevention have not been identified. We have previously reported that C-peptide prevents diabetic vasculopathy by inhibiting reactive oxygen species (ROS)-mediated endo...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-0039
更新日期:2013-11-01 00:00:00
abstract::Stimulation of cardiomyocyte guanosine 3',5'-cyclic monophosphate (cyclic GMP) via endothelial-derived nitric oxide (NO) is an important mechanism by which bradykinin and ACE inhibitors prevent hypertrophy. Endothelial NO dysfunction and cardiac hypertrophy are morbid features of diabetes not entirely prevented by ACE...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.9.2389
更新日期:2003-09-01 00:00:00
abstract::Expansion of pancreatic β-cells is a key goal of diabetes research, yet induction of adult human β-cell replication has proven frustratingly difficult. In part, this reflects a lack of understanding of cell cycle control in the human β-cell. Here, we provide a comprehensive immunocytochemical "atlas" of G1/S control m...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-0777
更新日期:2013-07-01 00:00:00
abstract::The molecular mechanism by which some, but not all, variants of encephalomyocarditis (EMC) virus selectively infect pancreatic beta-cells in mice and induce IDDM has been an enigma for more than a decade. We report here that the binding site of the EMC viral capsid protein VP1 determines viral diabetogenicity. Recombi...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.47.4.576
更新日期:1998-04-01 00:00:00
abstract::Many individuals with diabetes experience impaired cardiac contractility that cannot be explained by hypertension and atherosclerosis. This cardiomyopathy may be due to either organ-based damage, such as fibrosis, or to direct damage to cardiomyocytes. Reactive oxygen species (ROS) have been proposed to contribute to ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.3.777
更新日期:2003-03-01 00:00:00
abstract::We present a patient who developed insulin-requiring diabetes several years after the onset of symptoms of anterior hypopituitarism. It is likely that the hypopituitarism protected him against the development of diabetic retinopathy and glomerular basement membrane thickening but not against neuropathy and atheroma. T...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.24.11.1027
更新日期:1975-11-01 00:00:00
abstract::We explore whether racial differences in a United States population influence disease prevalence and perinatal outcome in gestational diabetes mellitus (GDM). The data presented are based on 3744 consecutive patients who underwent universal screening at 24-28 wk gestation; those with a 1-h plasma glucose greater than ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.40.2.s25
更新日期:1991-12-01 00:00:00
abstract::Thiazolidinediones and exercise are both known to improve insulin action independently. Therefore, we determined whether combined therapy could normalize insulin action in the Zucker fatty (ZF) rat. Rats were fed troglitazone as a 0.2% food admixture over a 3-week exercise training period (treadmill running 5 days/wee...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.12.2154
更新日期:2000-12-01 00:00:00
abstract::By closing ATP-sensitive K+ (K+-ATP) channels, glucose promotes depolarization-dependent Ca2+ entry and cytoplasmic free Ca2+ concentration ([Ca2+]i) rise in beta-cells. Ca2+-dependent exocytosis of insulin granules is then potentiated by a K+-ATP channel-independent action of glucose. The underlying mechanisms of thi...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.47.11.1713
更新日期:1998-11-01 00:00:00
abstract:OBJECTIVE:Haptoglobin (Hp) is upregulated in both inflammation and obesity. The low chronic inflammatory state, caused by massive adipose tissue macrophage (ATM) infiltration found in obesity, and low adiponectin have been implicated in the development of insulin resistance and hepatosteatosis. The aim of this work was...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-1536
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVE:We examined the relationship between the expression of thrombospondin (TSP)1, an antiangiogenic factor and regulator of transforming growth factor-beta activity, obesity, adipose inflammation, and insulin resistance. RESEARCH DESIGN AND METHODS:TSP1 gene expression was quantified in subcutaneous adipose tiss...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db07-0840
更新日期:2008-02-01 00:00:00
abstract::The effect of elevated glucagon concentrations on insulin requirements and on blood glucose concentrations was studied in five insulin-requiring diabetic subjects during feedback control of hyperglycemia with an automated glucose-controlled insulin infusion system (artificial endocrine pancreas) for six to eight hours...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.27.6.649
更新日期:1978-06-01 00:00:00
abstract::The effect of maternal diabetes on teratogenesis was examined utilizing female Chinese hamsters with typical diabetic symptoms from our closed colony (CHA) and F13-F19 of the brother-sister mating generations of a diabetic strain (CHAD). Both groups were mated with nondiabetic males from CHA. Embryologic studies on da...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.7.637
更新日期:1983-07-01 00:00:00
abstract::Not all patients with type 2 diabetes develop renal dysfunction. Identifying those at risk is problematic because even microalbuminuria, often used clinically as an indicator of future renal dysfunction, does not always precede worsening renal function. We sought to identify clinical risk factors at diagnosis of type ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db05-1620
更新日期:2006-06-01 00:00:00
abstract::The decline of leptin (Ob protein) concentrations during fasting is implicated as a signal for increasing the expression of the orexigenic peptide neuropeptide Y (NPY) in the hypothalamus. To test the hypothesis that the effects of food intake on arcuate nucleus NPY activation are mediated by leptin, we performed simu...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.48.4.828
更新日期:1999-04-01 00:00:00
abstract::Development of a robust insulin secretory response to glucose occurs during the early neonatal period. To determine if neuroendocrine agents play a role during this time, we studied the effects of selected peptides and neurotransmitters on insulin release and polyphosphoinositide metabolism in islets isolated from 1- ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.11.1337
更新日期:1989-11-01 00:00:00
abstract::We assessed our speculation that 2-cyclohexen-1-one (CHX) impairs glucose-induced insulin secretion through inactivation of glucokinase. Treatment of pancreatic islets with CHX at concentrations (0-5 mM) that caused a dose-dependent inactivation of glucokinase activity similarly inhibited glucose-induced insulin secre...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.39.10.1170
更新日期:1990-10-01 00:00:00