Secretory and viral fusion may share mechanistic events with fusion between curved lipid bilayers.

abstract：:The gene encoding the glycolytic enzyme triose-phosphate isomerase (TPI; EC 5.3.1.1) has been central to the long-standing controversy on the origin and evolutionary significance of spliceosomal introns by virtue of its pivotal support for the introns-early view, or exon theory of genes. Putative correlations between ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Logsdon JM Jr,Tyshenko MG,Dixon C,D-Jafari J,Walker VK,Palmer JD

更新日期：1995-08-29 00:00:00

abstract：:We discuss the pulling force f required to extrude a lipid tube from a living cell as a function of the extrusion velocity L. The main feature is membrane friction on the cytoskeleton. As recently observed for neutrophils, the tether force exhibits a "shear thinning" response over a large range of pulling velocities, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brochard-Wyart F,Borghi N,Cuvelier D,Nassoy P

更新日期：2006-05-16 00:00:00

abstract：:(3)H-actinomycin D, a guanine-binding agent, labels fixed human chromosomes nonrandomly. Actinomycin D added in G2 inhibits secondary constrictions and breaks chromosomes. There is some tendency for label to be concentrated at the ends of chromosomes and near the centromere. Labeling with (3)H-thymidine in the late st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles CP

更新日期：1970-03-01 00:00:00

abstract：:The development of osteoclastic cell lines would greatly facilitate analysis of the cellular and molecular biology of bone resorption. Several cell lines have previously been reported to be capable of osteoclastic differentiation. However, such cell lines form at best only occasional excavations, suggesting that osteo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chambers TJ,Owens JM,Hattersley G,Jat PS,Noble MD

更新日期：1993-06-15 00:00:00

abstract：:Recent scholarship suggests that the genomes of those around us affect our own phenotypes. Much of the empirical evidence for such "metagenomic" effects comes from animal studies, where the socio-genetic environment can be easily manipulated. Among humans, it is more difficult to identify such effects given the nonran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sotoudeh R,Harris KM,Conley D

更新日期：2019-08-13 00:00:00

abstract：:Renal cells isolated from vitamin D-deficient chicks had an increased Na+-dependent phosphate uptake when preincubated with 1,25-dihydroxycholecalciferol [1,25-(OH)2D3]. Phosphate uptake in the absence of Na+ and methyl alpha-glucoside uptake dependent on Na+ were not affected. Phosphate uptake was stimulated 15% by 0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang CT,Barnes J,Balakir R,Cheng L,Sacktor B

更新日期：1982-06-01 00:00:00

abstract：:The output of the cerebellar cortex is controlled by two main inputs, (i.e., the climbing fiber and mossy fiber-parallel fiber pathway) and activations of these inputs elicit characteristic effects in its Purkinje cells: that is, the so-called complex spikes and simple spikes. Target neurons of the Purkinje cells in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoebeek FE,Witter L,Ruigrok TJ,De Zeeuw CI

更新日期：2010-05-04 00:00:00

abstract：:The majority of outer membrane proteins (OMPs) from gram-negative bacteria and many of mitochondria and chloroplasts are β-barrels. Insertion and assembly of these proteins are catalyzed by the Omp85 protein family in a seemingly conserved process. All members of this family exhibit a characteristic N-terminal polypep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sommer MS,Daum B,Gross LE,Weis BL,Mirus O,Abram L,Maier UG,Kühlbrandt W,Schleiff E

更新日期：2011-08-16 00:00:00

abstract：:The Mauer mandible, holotype of Homo heidelbergensis, was found in 1907 in fluvial sands deposited by the Neckar River 10 km southeast of Heidelberg, Germany. The fossil is an important key to understanding early human occupation of Europe north of the Alps. Given the associated mammal fauna and the geological context...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wagner GA,Krbetschek M,Degering D,Bahain JJ,Shao Q,Falguères C,Voinchet P,Dolo JM,Garcia T,Rightmire GP

更新日期：2010-11-16 00:00:00

abstract：:Pancreatic islets regulate glucose homeostasis through coordinated actions of hormone-secreting cells. What underlies the function of the islet as a unit is the close approximation and communication among heterogeneous cell populations, but the structural mediators of islet cellular cross talk remain incompletely char...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes JW,Cho JH,Conway HE,DiGruccio MR,Ng XW,Roseman HF,Abreu D,Urano F,Piston DW

更新日期：2020-04-21 00:00:00

abstract：:Three preparations of purified papain-solublized HL-A antigens have been radiolabeled by reductive methylation using formaldehyde and potassium boro[3H]hydride, and their reaction with specific HL-A antisera has been investigated. Greater than 99 percent of the radioactivity in the [3H]HL-A2 preparation could be compl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parham P,Terhorst C,Herrmann H,Humphreys RE,Waterfield MD,Strominger JL

更新日期：1975-04-01 00:00:00

abstract：:Genetic potentials (pedigree-estimated breeding value) for milk and for fat were compared in cows grouped according to subclinical stage of bovine leukemia virus infection. Genetic potential for milk production was significantly greater in seropositive cows with persistent lymphocytosis (622 +/- 72 kg) and in seroposi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu MC,Shanks RD,Lewin HA

更新日期：1989-02-01 00:00:00

abstract：:Hearing is one of the last sensory modalities to be subjected to genetic analysis in Drosophila melanogaster. We describe a behavioral assay for auditory function involving courtship among groups of males triggered by the pulse component of the courtship song. In a mutagenesis screen for mutations that disrupt the aud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eberl DF,Duyk GM,Perrimon N

更新日期：1997-12-23 00:00:00

abstract：:Patients with systemic lupus erythematosus (SLE) have accelerated atherosclerosis. The underlying mechanisms are poorly understood, and investigations have been hampered by the absence of animal models that reflect the human condition of generalized atherosclerosis and lupus. We addressed this problem by transferring ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stanic AK,Stein CM,Morgan AC,Fazio S,Linton MF,Wakeland EK,Olsen NJ,Major AS

更新日期：2006-05-02 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were exposed to various thiol-directed spin labels, and the position of the label on the inner or outer vesicle surface was investigated as a function of the ATPase (adenosinetriphosphatase; ATP phosphohydrolase, EC 3.6.1.3) chemical state. Previous measurements of label accessibility t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Champeil P,Rigaud JL,Gary-Bobo CM

更新日期：1980-05-01 00:00:00

abstract：:Plants adapt to alterations in light conditions by controlling their gene expression profiles. Expression of light-inducible genes is transcriptionally induced by transcription factors such as HY5. However, few detailed analyses have been carried out on the control of transcription start sites (TSSs). Of the various w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kurihara Y,Makita Y,Kawashima M,Fujita T,Iwasaki S,Matsui M

更新日期：2018-07-24 00:00:00

abstract：:Calpains are Ca(2+)-dependent intracellular proteases. We show here that calpain-generated natural C-terminal fragments of proteins that include G protein-coupled receptors, transmembrane ion channels, transcriptional regulators, apoptosis controllers, kinases, and phosphatases (Phe-GluN2a, Lys-Ica512, Arg-Ankrd2, Tyr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Piatkov KI,Oh JH,Liu Y,Varshavsky A

更新日期：2014-03-04 00:00:00

abstract：:Classical eyeblink conditioning is a well-characterized model paradigm that engages the septohippocampal cholinergic system. This form of associative learning is impaired in normal aging and severely disrupted in Alzheimer's disease (AD). Some nicotinic cholinergic receptor subtypes are lost in AD, making the use of n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woodruff-Pak DS,Vogel RW 3rd,Wenk GL

更新日期：2001-02-13 00:00:00

abstract：:The mechanism by which antifreeze proteins (AFPs) irreversibly bind to ice has not yet been resolved. The ice-binding site of an AFP is relatively hydrophobic, but also contains many potential hydrogen bond donors/acceptors. The extent to which hydrogen bonding and the hydrophobic effect contribute to ice binding has ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garnham CP,Campbell RL,Davies PL

更新日期：2011-05-03 00:00:00

abstract：:The 1,3-1,4-beta-glucanase from Bacillus macerans (wtGLU) and the 1, 4-beta-xylanase from Bacillus subtilis (wtXYN) are both single-domain jellyroll proteins catalyzing similar enzymatic reactions. In the fusion protein GluXyn-1, the two proteins are joined by insertion of the entire XYN domain into a surface loop of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aÿ J,Götz F,Borriss R,Heinemann U

更新日期：1998-06-09 00:00:00

abstract：:Intrinsic genetic instability of RNA viruses may lead to the accumulation of revertants during manufacture of live viral vaccines, requiring rigorous quality control to ensure vaccine safety. Each lot of oral poliovirus vaccine (OPV) is tested for neurovirulence in animals and also for the presence of neurovirulent re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neverov A,Chumakov K

更新日期：2010-11-16 00:00:00

abstract：:Phycobilisomes isolated from eight different species of cyanobacteria contain in addition to the light-harvesting phycobiliproteins, a small number of colorless polypeptides with molecular weights higher than those of the chromopolypeptide subunits of the phycobiliproteins. In the phycobilisomes of the species examin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Marsac NT,Cohen-bazire G

更新日期：1977-04-01 00:00:00

abstract：:Smad proteins are key intracellular signaling effectors for the transforming growth factor-beta superfamily of peptide growth factors. Following receptor-induced activation, Smads move into the nucleus to activate transcription of a select set of target genes. The activity of Smad proteins must be tightly regulated to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Chang C,Gehling DJ,Hemmati-Brivanlou A,Derynck R

更新日期：2001-01-30 00:00:00

abstract：:Biosynthesis of sucrose-6-P catalyzed by sucrose-phosphate synthase (SPS), and the presence of sucrose-phosphate phosphatase (SPP) leading to the formation of sucrose, have both been ascertained in a prokaryotic organism: Anabaena 7119, a filamentous heterocystic cyanobacterium. Two SPS activities (SPS-I and SPS-II) w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Porchia AC,Salerno GL

更新日期：1996-11-26 00:00:00

abstract：:Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park GT,Frost JM,Park JS,Kim TH,Lee JS,Oh SA,Twell D,Brooks JS,Fischer RL,Choi Y

更新日期：2014-12-23 00:00:00

abstract：:Fully atomistic molecular-dynamics (MD) simulations of perfluoroalkylalkane molecules at the surface of water show the spontaneous formation of aggregates whose size and topography closely resemble the experimentally observed hemimicelles for this system. Furthermore, the grazing incidence X-ray diffraction (GIXD) pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silva GMC,Morgado P,Lourenço P,Goldmann M,Filipe EJM

更新日期：2019-07-23 00:00:00

abstract：:We compared students' self-reported perception of learning with their actual learning under controlled conditions in large-enrollment introductory college physics courses taught using 1) active instruction (following best practices in the discipline) and 2) passive instruction (lectures by experienced and highly rated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deslauriers L,McCarty LS,Miller K,Callaghan K,Kestin G

更新日期：2019-09-24 00:00:00

abstract：:Creative cognition requires mental exploration of remotely connected concepts while suppressing dominant ones. Across four experiments using different samples of participants, we provide evidence that right temporal alpha oscillations play a crucial role in inhibiting habitual thinking modes, thereby paving the way fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luft CDB,Zioga I,Thompson NM,Banissy MJ,Bhattacharya J

更新日期：2018-12-26 00:00:00

abstract：:Transgenic mice expressing the sequences coding for the envelope proteins of the hepatitis B virus (HBV) in the liver have been used as a model of the HBV chronic carrier state. We evaluated the possibility of inducing a specific immune response to the viral envelope antigens and thus potentially controlling chronic H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mancini M,Hadchouel M,Davis HL,Whalen RG,Tiollais P,Michel ML

更新日期：1996-10-29 00:00:00

abstract：:If X(1), X(2),... are independent random variables with zero expectation and finite variances, the cumulative sums S(n) are, on the average, of the order of magnitude S(n), where S(n) (2) = E(S(n) (2)). The occasional maxima of the ratios S(n)/S(n) are surprisingly large and the problem is to estimate the extent of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feller W

更新日期：1969-07-01 00:00:00