Abstract:
BACKGROUND:The purpose of this study was to further characterize the immune response to Mycobacterium tuberculosis (Mtb) antigens, in order to provide new insight into host-pathogen interactions in tuberculosis (TB), and to offer tools for a more accurate diagnosis of the different stages of TB. METHODS:T-cell responses to Bacillus Calmette-Guérin (BCG), purified protein derivative (PPD), early secretory antigenic target-6 (ESAT-6) protein and culture filtrate protein-10 kDa (CFP-10) were measured in terms of interferon (IFN)-gamma and interleukin (IL)-2 release, using a novel flow cytometric cell-secreting cytokine detection technique. The study was conducted on peripheral blood mononuclear cells (PBMC) obtained from active TB patients, latently TB infected individuals, and healthy donors. IL-10 and IL-17 were also measured to test their possible role as indicators of disease activity. RESULTS:We confirmed that the enumeration of IFN-gamma releasing cells upon Mtb-specific stimulation is sufficient to identify TB patients and that CD8+ T cells concur to IFN-gamma secretion. IL-2 secreting cells were more frequently observed in latent TB infected individuals compared to active TB patients, suggesting that measurement of cells secreting this cytokine could be a marker of disease stage. No discriminating role was associated to IL-10 and IL-17 release in TB patients. CONCLUSION:Our data indicate that the flow cytometric cytokine-secreting cell detection technique may be envisaged as an additional tool for TB diagnosis allowing the analysis of the immune response to M. tuberculosis-related antigens in the different stages of TB.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Sargentini V,Mariotti S,Carrara S,Gagliardi MC,Teloni R,Goletti D,Nisini Rdoi
10.1186/1471-2334-9-99subject
Has Abstractpub_date
2009-06-23 00:00:00pages
99issn
1471-2334pii
1471-2334-9-99journal_volume
9pub_type
杂志文章abstract:BACKGROUND:Compared to methicillin-resistant Staphylococcus aureus (MRSA), characteristics of nasal carriage and community-onset infection methicillin-susceptible S. aureus (MSSA) are less well known. No characteristics of MSSA in Taiwan have been reported previously. METHODS:We analyzed 100 nasal carriage and 34 comm...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-343
更新日期:2012-12-10 00:00:00
abstract:BACKGROUND:Identification of people living with HIV is key in HIV prevention and control. Partner Notification service is a World Health Organization backed strategy of reaching out to sexual partners of people diagnosed with HIV for HIV testing. However, its adoption and success rate in Kenya remains unknown. METHODS...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05146-9
更新日期:2020-06-22 00:00:00
abstract:BACKGROUND:In intensive care unit (ICU), infection and colonization by resistant Gram-negative bacteria increase costs, length of stay and mortality. Extended-spectrum beta-lactamase--producing Enterobacteriaceae (ESBL-E) is a group of pathogens increasingly encountered in ICU setting. Conditions that promote ESBL-E ac...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1489-z
更新日期:2016-04-13 00:00:00
abstract:BACKGROUND:The first step of designing any genome-based molecular diagnostic test is to find a specific target sequence. The modified genome comparison method is one of the easiest and most comprehensive ways to achieve this goal. In this study, we aimed to explain this method with the example of Mycobacterium tubercul...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3417-x
更新日期:2018-10-12 00:00:00
abstract:BACKGROUND:Severe infection is a main cause of mortality. We aim to describe risk factors for long-term mortality among inpatients with severe infection. METHODS:Prospective cohort study in a 600-bed university hospital in Portugal including all patients with severe infection admitted into intensive care, medical, sur...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3054-4
更新日期:2018-04-05 00:00:00
abstract:BACKGROUND:Central nervous system (CNS) infections are a significant contributor to morbidity and mortality globally. However, most published studies have been conducted in developed countries where the epidemiology and aetiology differ significantly from less developed areas. Additionally, there may be regional differ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1640-x
更新日期:2016-06-16 00:00:00
abstract:BACKGROUND:COVID-19 is novel infectious disease with an evolving understanding of its epidemiology and clinical manifestations. Severe cases developed life-threatening complications, such as respiratory failure, shock, and multiple organs dysfunction. Immunocompromised patients often present atypical presentations of v...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
doi:10.1186/s12879-020-05480-y
更新日期:2020-10-19 00:00:00
abstract:BACKGROUND:To explore the clinical significance of serum angiotensin-converting enzyme (ACE) activity in coronavirus disease 2019 (COVID-19). METHODS:In this retrospective study, a total of 136 consecutive patients with confirmed COVID-19 were recruited. Demographic and clinical data were recorded. The serum ACE activ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05619-x
更新日期:2020-11-25 00:00:00
abstract:BACKGROUND:The role of single nucleotide polymorphisms (SNPs) of interleukin (IL)-28B in predicting therapeutic response of pegylated interferon (peg-IFN) plus ribavirin (PR) for genotype 1 infected chronic hepatitis C patients with advanced fibrosis (AF) is limited. The aim of this study is to assess its role in predi...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0888-x
更新日期:2015-03-26 00:00:00
abstract:BACKGROUND:Bacterial vaginosis (BV) - a syndrome characterised by a shift in vaginal flora - appears to be particularly common in sub-Saharan Africa, but little is known of the pattern of vaginal flora associated with BV in Africa. We conducted a study aimed at determining the prevalence of BV and patterns of BV-associ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-5-12
更新日期:2005-03-09 00:00:00
abstract:BACKGROUND:Antibiotic resistant strains of Acinetobacter baumannii have been responsible for an increasing number of nosocomial infections including bacteremia and ventilator-associated pneumonia. In this study, we analyzed 38 isolates of A. baumannii obtained from two hospital outbreaks in Los Angeles County for the m...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1526-y
更新日期:2016-05-04 00:00:00
abstract:BACKGROUND:The spatial spread of H5N1 avian influenza, significant ongoing mutations, and long-term persistence of the virus in some geographic regions has had an enormous impact on the poultry industry and presents a serious threat to human health. METHODS:We applied phylogenetic analysis, geospatial techniques, and ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0770-x
更新日期:2015-02-13 00:00:00
abstract:BACKGROUND:HIV self-testing (HIVST) can be performed using directly assisted and unassisted approaches in facilities or communities to reach different populations. The aim of this study was to compare the practicability and effectiveness of the two delivery approaches for HIVST, unassisted HIVST (UH) and directly assis...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12879-020-05554-x
更新日期:2020-11-11 00:00:00
abstract:BACKGROUND:This study presents an analysis of 1,491 serogroup 23 and 762 serogroup 15 isolates from invasive pneumococcal disease (IPD) in children and adults before and after the general recommendation for childhood pneumococcal conjugate vaccination in Germany in July 2006. Vaccination formulations used were PCV7 (fr...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0941-9
更新日期:2015-05-05 00:00:00
abstract:BACKGROUND:Infection by Staphylococcus aureus (S. aureus) is a major cause of morbidity and mortality. Colonization by S. aureus increases the risk of infection. Little is known about decolonization strategies for S. aureus beyond antibiotics, however probiotics represent a promising alternative. A randomized controlle...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12879-018-3028-6
更新日期:2018-03-14 00:00:00
abstract:BACKGROUND:There is little information on nasopharyngeal (NP) flora or bacteremia in HIV-infected children. Our aim was to describe the organisms and antimicrobial resistance patterns in children enrolled in a prospective study comparing daily and three times weekly trimethoprim-sulfamethoxazole (TMP-SMX) and isoniazid...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-40
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:Group B streptococcus (GBS) is reported as the leading cause of neonatal sepsis and meningitis. Newborns from GBS colonized pregnant women are at high risk of infection. METHOD:A Hospital based cross-sectional study was conducted at Hawassa University Comprehensive Specialized Hospital from November 05, 201...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3859-9
更新日期:2019-04-16 00:00:00
abstract:BACKGROUND:Tumor necrosis factor alpha (TNF-α) plays a key role in the containment of tuberculosis. The relationship between the TNF -238G/A polymorphism and tuberculosis susceptibility remains inconclusive. A comprehensive meta-analysis was made to provide a more precise estimate of the relationship between them. MET...
journal_title:BMC infectious diseases
pub_type: 杂志文章,meta分析
doi:10.1186/1471-2334-12-328
更新日期:2012-11-28 00:00:00
abstract:BACKGROUND:Models of infectious disease are increasingly utilising empirical contact data to quantify the number of potentially infectious contacts between age groups. While a growing body of data is being collected on contact patterns across many populations, less attention has been paid to the social contacts of youn...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2735-8
更新日期:2017-09-20 00:00:00
abstract:BACKGROUND:The management of disseminated cysticercosis is unclear and largely considered hazardous. The role of albendazole remains controversial in such patients. METHODS:A tertiary care, University hospital-based prospective intervention study was conducted from December 2015 to December 2017. Patients with dissemi...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-4891-5
更新日期:2020-03-14 00:00:00
abstract:BACKGROUND:Bloodstream infection (BSI) is one of the major causes of morbidity and mortality for patients undergoing hematopoietic stem cell transplantation (HSCT). The unrelated cord blood transplantation (UCBT) can provided opportunities for patients without suitable donors for bone marrow transplantation (BMT) and p...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3575-x
更新日期:2018-12-13 00:00:00
abstract:BACKGROUND:Human cytomegalovirus (CMV) is a common herpesvirus which is estimated to infect 83% of the global population. Whilst many infections are asymptomatic, it is an important cause of morbidity and mortality, particularly for immunocompromised people and for infants who are congenitally infected. A vaccine again...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05572-9
更新日期:2020-11-23 00:00:00
abstract:BACKGROUND:Chronic Q fever usually presents as endocarditis or endovascular infection. We investigated whether 18F-FDG PET/CT and echocardiography were able to detect the localization of infection. Also, the utility of the modified Duke criteria was assessed. METHODS:Fifty-two patients, who had an IgG titre of ≥ 1024...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-413
更新日期:2013-09-03 00:00:00
abstract:BACKGROUND:Under-reporting of tuberculosis (TB) cases complicates disease control, hinders contact tracing and alters the accuracy of epidemiological data, including disease burden. The objective of the present study is to evaluate the proportion of unreported TB cases in Spanish healthcare facilities and to identify t...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1047-0
更新日期:2015-07-29 00:00:00
abstract:BACKGROUND:Understanding the underlying epidemiology that shapes Neisseria gonorrhoeae (GC), and Chlamydia trachomatis (CT) infections can contribute to data driven policies directed towards curbing the proliferation of these pathogens in Ghana. Information on the symptoms and risk factors for STIs will help to identif...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4035-y
更新日期:2019-05-16 00:00:00
abstract:BACKGROUND:In order to compare the transmissibility of the 2009 pH1N1 pandemic during successive waves of infections in summer and fall/winter in the Northern Hemisphere, and to assess the temporal changes during the course of the outbreak in relation to the intervention measures implemented, we analyze the epidemiolog...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-332
更新日期:2011-12-02 00:00:00
abstract:BACKGROUND:Enterovirus 71 (EV-A71) shows a potential of rapid death, but the natural history of the infection is poorly known. This study aimed to examine the natural history of EV-A71 infection. METHODS:This was a prospective longitudinal observational study performed between January 1st and October 31st, 2012, at th...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究
doi:10.1186/s12879-017-2228-9
更新日期:2017-02-17 00:00:00
abstract:BACKGROUND:Contact surveys and diaries have conventionally been used to measure contact networks in different settings for elucidating infectious disease transmission dynamics of respiratory infections. More recently, technological advances have permitted the use of wireless sensor devices, which can be worn by individ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-136
更新日期:2014-03-10 00:00:00
abstract:BACKGROUND:Guidelines from the Infectious Diseases Society of America/The American Thoracic Society (IDSA/ATS) provide recommendations for diagnosis and treatment of ventilator-associated pneumonia (VAP). However, the mere presence of guidelines is rarely sufficient to promote widespread adoption and uptake. Using the ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1665-1
更新日期:2016-07-22 00:00:00
abstract:BACKGROUND:No comparison data have been reported on viral and epidemiological profiles of hospitalized children with severe acute respiratory infection (SARI) in Beijing or Shanghai, China. METHODS:We collected 700 nasopharyngeal aspirates (NPA) from hospitalized children with SARI in Beijing (northern China) and Shan...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4385-5
更新日期:2019-08-20 00:00:00