Influence of bacteria and leukocytes on the outcome of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).

Abstract:

:The influence of bacteria and/or leukocytes on the outcome of IVF or ICSI is influenced by three factors which have little in common with in vivo conditions: 1) The process of ejaculate preparation (swim-up, Percoll) with antibiotic buffered media; 2) The small amount of inseminated spermatozoa (100,000 per culture); 3) The short cultivation time. From the very beginning, these factors limit whatever the influence of bacteria and leukocytes on fertilization and embryonic development in vivo may be. Despite the contradictory results published so far, the influence of bacteria and/or leukocytes on the functional integrity of spermatozoa during the process of IVF or ICSI can be ignored. Furthermore, during IVF or ICSI the spermatozoon does not act as a vector for the transportation of bacteria into the ooplasm.

journal_name

Andrologia

journal_title

Andrologia

authors

Michelmann HW

doi

10.1111/j.1439-0272.1998.tb02833.x

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

99-101

eissn

0303-4569

issn

1439-0272

journal_volume

30 Suppl 1

pub_type

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