Abstract:
:The influence of bacteria and/or leukocytes on the outcome of IVF or ICSI is influenced by three factors which have little in common with in vivo conditions: 1) The process of ejaculate preparation (swim-up, Percoll) with antibiotic buffered media; 2) The small amount of inseminated spermatozoa (100,000 per culture); 3) The short cultivation time. From the very beginning, these factors limit whatever the influence of bacteria and leukocytes on fertilization and embryonic development in vivo may be. Despite the contradictory results published so far, the influence of bacteria and/or leukocytes on the functional integrity of spermatozoa during the process of IVF or ICSI can be ignored. Furthermore, during IVF or ICSI the spermatozoon does not act as a vector for the transportation of bacteria into the ooplasm.
journal_name
Andrologiajournal_title
Andrologiaauthors
Michelmann HWdoi
10.1111/j.1439-0272.1998.tb02833.xsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
99-101eissn
0303-4569issn
1439-0272journal_volume
30 Suppl 1pub_type
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