Abstract:
:The events occurring at the maternal-foetal interface define a successful pregnancy but the current paradigm has shifted towards assessing the contribution of spermatozoa for embryogenesis. Spermatozoa with defective DNA integrity may fertilise the oocyte but affect subsequent embryonic development. The present case-control study was conducted in male partners of couples experiencing recurrent pregnancy loss (RPL) to assess the gene expression of spermatozoal FOXG1, SOX3, OGG1, PARP1, RPS6, RBM9, RPS17 and RPL29. This was correlated with reactive oxygen species (ROS) levels and DNA Fragmentation Index (DFI). Semen samples were obtained from 60 cases and 30 fertile controls. Gene expression was done by qPCR analysis, and relative quantification was calculated by the 2-ΔΔCt method. Chemiluminescence and the sperm chromatin structure assay were used to measure the ROS and DFI levels respectively. FOXG1, OGG1, RPS6 and RBM9 were seen to be upregulated, while SOX3 and PARP1 were downregulated. Relative expression of SOX3, OGG1, RPS6 and RPS17 showed a significant difference between patients and controls (p < 0.05). RPL patients were seen to have high ROS (>27.8; p = 0.001) and DFI (>30.7; p < 0.0001) with respect to controls. Sperm transcript dysregulation and oxidative DNA damage can be "carried over" after implantation, thus affecting embryogenesis and health of the future progeny.
journal_name
Andrologiajournal_title
Andrologiaauthors
Dhawan V,Kumar M,Deka D,Malhotra N,Singh N,Dadhwal V,Dada Rdoi
10.1111/and.13171subject
Has Abstractpub_date
2019-02-01 00:00:00pages
e13171issue
1eissn
0303-4569issn
1439-0272journal_volume
51pub_type
杂志文章相关文献
ANDROLOGIA文献大全abstract::Anatomical endoscopic enucleation of the prostate (AEEP) differs from other surgical techniques for benign prostatic obstruction (BPO) in that it removes the entire benign prostatic hyperplasia (BPH) component of the prostate. We summarise the main advantages of AEEP compared to other surgical techniques for BPO. Thes...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/and.13643
更新日期:2020-09-01 00:00:00
abstract::Polyorchidism is defined as the presence of more than 2 histologically proven testes. We report the case of a 20-year-old man with polyorchidism, presenting with right scrotal pain and right scrotal ovoid mass. Scrotal examination revealed two ovoid, mobile lumps with testicular sensation in the right side of the scro...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.2011.01205.x
更新日期:2012-05-01 00:00:00
abstract::This study was aimed at determining the effects of corn and wheat gluten, used as dietary protein sources, on live weight gain, sperm quality and the histology of the testes and accessory glands in male rats. For this purpose, 20-day-old 24 male Wistar albino rats were randomly assigned to three groups. Group 1 (Contr...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13601
更新日期:2020-07-01 00:00:00
abstract::This study was performed to investigate the effect of propylthiouracil (PTU) on rat testis, and to compare the results of the different treatment regimens of levothyroxine and zinc. Twenty sexually mature Wistar albino rats were subjected to PTU for 14 days intraperitoneally to make them hypothyroidic. The effect of P...
journal_title:Andrologia
pub_type: 杂志文章,收录出版
doi:10.1046/j.1439-0272.2000.00324.x
更新日期:2000-03-01 00:00:00
abstract::The incretory testicular function was tested in 21 patient with varicocele aged 19 to 39 years. In one group baseline testosterone was measured, while a second group received a single injection of 5000 IU HCG. Testosterone was studied in peripheral plasma in the spermatic vein and in testicular tissue. Baseline values...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1983.tb00179.x
更新日期:1983-11-01 00:00:00
abstract::In the present study, our experience in the collection of semen from adult bonnet monkeys and some of the characteristics of semen are reported. Semen samples were collected by penile stimulation every 15 days over a period of 3 years. The penile method of ejaculation was more consistent with an increased volume of se...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1998.tb01153.x
更新日期:1998-03-01 00:00:00
abstract::A careful history and evaluation of men with chronic orchialgia elucidates the aetiology in some men to be a hyperactive cremaster muscle reflex with testicular retraction as the cause. The objective is to evaluate outcomes in men who underwent microsurgical subinguinal cremaster muscle release (MSCMR) with a retrospe...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13493
更新日期:2020-02-01 00:00:00
abstract::In fertile men, the majority of epididymal spermatozoa acquire the potential to fertilize (assessed with sperm function assays) on passage into the corpus and cauda regions of the epididymis. Although secretions of the epididymal epithelium are clearly important for sperm maturation and survival, their role in this pr...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.1998.tb01165.x
更新日期:1998-08-01 00:00:00
abstract::Testicular torsion is a urology urgent disease which causes testicular injury and potential sterility. In this study, we explored the protective influence of rosiglitazone on testicular ischaemia-reperfusion damage. There were 28 male Sprague Dawley rats in total, which were assigned randomly to four groups. Group A w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12947
更新日期:2018-01-09 00:00:00
abstract::In a prospective study, the polymorphism of oestrogen receptor β gene was investigated in nonobstructive azoospermia patients. Ninety infertile patients with nonobstructive azoospermia diagnosed after two semen analysis, 2 weeks apart and negative testicular sperm extraction during intracytoplasmic sperm injection, an...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12575
更新日期:2016-12-01 00:00:00
abstract::The role of opioidergic system in controlling gonadotropin secretion in elderly men still remains uncertain. In the present study, we attempted to examine the opioidergic inhibitory tone imposed on GnRH release mechanism in elderly men by monitoring the plasma gonadotropin response to the opiate receptor antagonist na...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1994.tb00752.x
更新日期:1994-01-01 00:00:00
abstract::Based on the synchronization between a periodic vibration and flashing light, we designed a device to determine the flagellar beating frequency of human sperm. The head of a spermatozoon was either held by the tip of a micropipette operated with a micromanipulator or adhered by itself on the surface of a glass slide w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1998.tb01382.x
更新日期:1998-02-01 00:00:00
abstract::Primordial germ cells in the mouse are known to be derived from the epiblast. They can be identified histochemically, by their high alkaline phosphatase activity. At 8 d post coitum they have been observed within the embryonic part of the egg cylinder, at the posterior end of the primitive streak. Earlier, at 7.25 day...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.1992.tb02647.x
更新日期:1992-09-01 00:00:00
abstract::The present study was conducted to evaluate in vitro and in vivo antiproliferative potential of the Cameroonian propolis and to elucidate its underlying mechanism. In vitro, ethanol-extracted propolis (EEP) was tested on cell growth, cell proliferation, cell cycle, cell death mechanism and cell migration. The cell cyc...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13698
更新日期:2020-10-01 00:00:00
abstract::The total amount of humoral sperm antibody protein is determined following its isolation by affinity-chromatography on spermatozoa. Serum proteins are eluted by Sorensen phosphate buffer. Antibodies attached to spermatozoa suspended in Sephadex G 75 columns resist elution by Sorensen buffer. They can be quantitatively...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1975.tb01236.x
更新日期:1975-01-01 00:00:00
abstract::Immunohistochemical and morphological examination of 50 bilateral testicular biopsies of infertile men shows the presence of immunoglobulin in 20% of the cases, suggesting that testicular immune phenomena might be implicated in the pathogenesis of infertility. Several observations make it clear that immune phenomena a...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1980.tb00594.x
更新日期:1980-03-01 00:00:00
abstract::Over the past decades, there is an increasing number of association studies of telomere length (TL) and the risk and recurrence of prostate cancer (PCa), but the results are inconsistent. Hence, we identify the relevant studies published in English on or before 10 January 2019 conducting a literature review in the ele...
journal_title:Andrologia
pub_type: 杂志文章,meta分析
doi:10.1111/and.13304
更新日期:2019-08-01 00:00:00
abstract::In the present study, we aimed to evaluate the effects of dehydroepiandrosterone (DHEA) on apoptosis of testicular germ cells after repair of testicular torsion in rats. Twenty-four adult male Sprague-Dawley rats were randomly divided into four groups, with six rats in each group: sham operation, torsion/detorsion (T/...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2008.00806.x
更新日期:2008-02-01 00:00:00
abstract::Microscopy has been used in the routine evaluation of sperm metabolism. Nevertheless, it has limited capacity to preview male fertility. As calorimetry may be used to evaluate directly the metabolic activity of a biological system, the aim of this study was to use microcalorimetry as an additive method for sperm metab...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2009.00963.x
更新日期:2010-04-01 00:00:00
abstract::The distribution of the main lectin receptors of boar spermatozoa were analyzed by concanavalin A and Ricinus communis agglutinin-120 visualized for light microscopy by enzymatic amplification with 3-amino-9-ethylcarbazole. The lectin binding sites of the acrosomal membranes in addition to the plasma membrane were dis...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Gamma-glutamyl transpeptidase was purified to apparent homogeneity from human testis by DEAE cellulose, acetone, precipitation, fractionation by ammonium sulphate, Sephacryl S-200 chromatography, Q-Sepharose chromatography and S-Sepharose chromatography following solubilization of the enzyme by Triton X-100. The purif...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1990.tb01973.x
更新日期:1990-05-01 00:00:00
abstract::Male infertility is a major health problem worldwide. We investigated a possible association between leptin, obesity, hormonal interplay and male infertility. This cross-sectional study of 313 males (178 infertile and 135 fertile) was carried out in 2017. The subjects were categorised by body mass index (BMI) and body...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13147
更新日期:2019-02-01 00:00:00
abstract::This study aimed to examine the association of herpes zoster (HZ) with androgen deprivation therapy (ADT) use among patients with prostate cancer (PC), using a population-based data set. The study sample for this study was retrieved from the Taiwan Longitudinal Health Insurance Database 2005. We selected 877 patients ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12863
更新日期:2018-03-01 00:00:00
abstract::Genetic factors that could mediate the pathogenesis of male reproductive disorders are largely unclear. Polymorphisms of cytochrome P4501A1 (CYP1A1), a key enzyme in the extrahepatic metabolism of lipophilic xenobiotics, have been shown to influence susceptibility to xenobiotics. Here, CYP1A1 polymorphisms were invest...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1998.tb01387.x
更新日期:1998-05-01 00:00:00
abstract::High-fat diets (HFDs) are detrimental to steroidogenesis and male fertility. This study aimed to investigate the protective effects of melatonin (MT) treatment on testicular dysfunction in mice fed with HFD. C57BL/6J male mice were randomly divided into three groups: CTRL, HFD and HFD + MT. MT treatment mitigated the ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13323
更新日期:2019-09-01 00:00:00
abstract::In order to investigate the role of testosterone propionate (TP) on the antioxidant system of the rat testis, lipid peroxidation (LPX) and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of the testis of testosterone-treated and control rats were compared. The results indicate...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1997.tb00328.x
更新日期:1997-11-01 00:00:00
abstract::For patients with threatened fertility, preservation of it is a major concern. Although promising results have been obtained in animal models using testicular germ cell suspensions, in humans, it is crucial to first develop an efficient method of cryopreservation to be able to apply to transplantation. Thus, four reli...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2012.01290.x
更新日期:2012-12-01 00:00:00
abstract::Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a multifactorial problem affecting men of all ages and demographics. Currently, there is a relative dearth of epidemiological information on CPPS. It is clear that patients with CPPS have a dismal quality of life and many have benefited only minimally from ...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1046/j.1439-0272.2003.00584.x
更新日期:2003-10-01 00:00:00
abstract::Using a highly sensitive radioimmunoassay (RIA) of estramustine binding protein (EMBP) with the antibody raised against rat-EMBP, the concentration of EMBP in human benign prostatic hypertrophy (BPH) tissue was evaluated. Using mechanical separation procedure, EMBP was found significantly higher in the epithelium (10....
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1990.tb01990.x
更新日期:1990-07-01 00:00:00
abstract::The inactivation of ornithine decarboxylase, a polyamine biosynthetic enzyme, by alpha-difluoromethylornithine (alpha-DFMO) was studied in patients with chronic non-suppurative prostatitis. Partial clinical response and remission of gross hematospermia were observed after 18 g/day alpha-DFMO, when administered orally ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1981.tb00014.x
更新日期:1981-03-01 00:00:00