Oestrogen receptors beta genotype in infertile Egyptian men with nonobstructive azoospermia.

Abstract:

:In a prospective study, the polymorphism of oestrogen receptor β gene was investigated in nonobstructive azoospermia patients. Ninety infertile patients with nonobstructive azoospermia diagnosed after two semen analysis, 2 weeks apart and negative testicular sperm extraction during intracytoplasmic sperm injection, and 60 fertile men as controls were enrolled in the study. Semen analysis, hormonal profile and allele-specific PCR reaction were performed to detect variants of the RsaI polymorphism of the oestrogen receptor β gene for all patients and controls. The mean patient's age was significantly lower than the mean age of the controls (P < 0.05). There was a significant increase in the mean serum levels of FSH, LH, free testosterone and E2 and significant decrease in total testosterone in patients than controls (P < 0.05). In the patients, the frequency of the homozygous GG, heterozygous AG and homozygous AA genotype was 83.3%, 14.3% and 3.3% respectively, whereas their frequencies in the controls were 95%, 5% and 0% respectively (odds ratio 3.8). There is no significant correlation between ERß polymorphisms and patient's age or pituitary and sex hormones (P > 0.05). Our findings suggested that in Egyptian population, genetic mutation in ERß is associated with the risk of nonobstructive azoospermia.

journal_name

Andrologia

journal_title

Andrologia

authors

Younes AH,Hamed HB,Mohamed EM,Makki MA,Gaber N,Mohamed HM

doi

10.1111/and.12575

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

1289-1293

issue

10

eissn

0303-4569

issn

1439-0272

journal_volume

48

pub_type

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