Swim-down separation of progressively motile sperm from poor quality human semen by the modified funnel procedure.

Abstract:

:The simultaneous swimming up and down from identical semen specimens suggested that the separation efficiency of progressively motile sperm by the swim-down method was superior to that of the swim-up method. The swimming down was performed in a small plastic funnel, the bottom of which was plugged with a 2.5 ml disposable syringe. In this funnel, 2.0 ml of 70% Percoll was poured and then the sperm resuspension was overlaid. After 1 h, progressively motile sperm penetrating into the lower part of Percoll layer (1.6 ml) was collected in the syringe. The technical strategies to yield higher density of progressively motile sperm were found to be centrifugal concentration of the sperm from whole ejaculate prior to the swimming down, and subsequent re-centrifugation of the separated sperm after the swimming down. Oligo-asthenozoospermic semen (22 +/- 4.4 x 10(6) ml1, 5.5 +/- 4.4% motility, n = 8) was processed by the present method, yielding sperm qualities of 32 +/- 19 x 10(6) ml1, 74 +/- 14% in the final preparations. Overall improvement in fertility index (sperm density ml1 x motility % x 10(8)) reached 27-folds on average.

journal_name

Andrologia

journal_title

Andrologia

authors

Kobayashi T,Kaneko S,Hara I,Aoki R,Ohno T,Nozawa S

doi

10.1111/j.1439-0272.1991.tb02485.x

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

17-20

issue

1

eissn

0303-4569

issn

1439-0272

journal_volume

23

pub_type

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