Partial cDNA sequence analysis of the boar proacrosin-binding protein.

Abstract:

:A partial cDNA sequence of the boar 28, 29-kDa proacrosin-binding protein was determined. The desired sequence was amplified and obtained by RT-PCR followed by nested PCR. The oligonucleotide primers for RT-PCR were designed by deduction from the internal amino acid sequence of the boar proacrosin-binding protein. An approximately 1-kbp DNA fragment was analysed by agarose gel electrophoresis, in which the boar testicular total RNA or poly(A)+ RNA was used as a template for RT-PCR followed by nested PCR. This PCR product was inserted into a TA vector. The DNA sequence of the inserted PCR product was then determined by the chain termination method.

journal_name

Andrologia

journal_title

Andrologia

authors

Huh K,Yi LS

doi

10.1046/j.1439-0272.2001.00466.x

keywords:

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

379-83

issue

6

eissn

0303-4569

issn

1439-0272

pii

466

journal_volume

33

pub_type

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