Abstract:
:One of the challenging features of energy-transducing terminal oxidases, like the aa3 cytochrome c oxidase of Paracoccus denitrificans, is the translocation of protons across the cytoplasmic membrane, which is coupled to the transfer of electrons to oxygen. As a prerequisite for a more advanced examination of the enzymatic properties, several amino acid residues, selected on the basis of recent three-dimensional structure determinations, were exchanged in subunit I of the Paracoccus enzyme by site-directed mutagenesis. The properties of the mutated oxidases were analyzed by different methods to elucidate whether they are involved in the coupled and coordinated transfer of protons via two different pathways either to the site of oxygen reduction or through the enzyme from the cytoplasm to the periplasmic side.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Pfitzner U,Odenwald A,Ostermann T,Weingard L,Ludwig B,Richter OMdoi
10.1023/a:1020515713103subject
Has Abstractpub_date
1998-02-01 00:00:00pages
89-97issue
1eissn
0145-479Xissn
1573-6881journal_volume
30pub_type
杂志文章,评审abstract::The inner membrane of yeast and mammalian mitochondria has been studied in situ with a patch clamp electrode. Anion channels were found in both cases, although their behavior and regulation are different. In mammalian mitochondria, the principal channel is of around 100 pS conductance and opens mainly under depolarize...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF02110642
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abstract::The presence of additional subunits in cytochrome oxidase distinguish the multicellular eukaryotic enzyme from that of a simple unicellular bacterial enzyme. The number of these additional subunits increases with increasing evolutionary stage of the organism. Subunits I-III of the eukaryotic enzyme are related to the ...
journal_title:Journal of bioenergetics and biomembranes
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abstract::The ATP synthase of the yeast Saccharomyces cerevisiae is composed of 20 different subunits whose primary structure is known. The organization of proteins that constitute the membranous domain is now under investigation. Cysteine insertions combined with the use of nonpermeant maleimide reagents and cross-linking reag...
journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
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journal_title:Journal of bioenergetics and biomembranes
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abstract::ATP1-111, a suppressor of the slow-growth phenotype of yme1Delta lacking mitochondrial DNA is due to the substitution of phenylalanine for valine at position 111 of the alpha-subunit of mitochondrial ATP synthase (Atp1p in yeast). The suppressing activity of ATP1-111 requires intact beta (Atp2p) and gamma (Atp3p) subu...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
abstract::Mitochondrial reactive oxygen species (ROS) production was investigated in mitochondria extracted from liver of rats treated with or without metformin, a mild inhibitor of respiratory chain complex 1 used in type 2 diabetes. A high rate of ROS production, fully suppressed by rotenone, was evidenced in non-phosphorylat...
journal_title:Journal of bioenergetics and biomembranes
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doi:10.1007/s10863-006-9003-8
更新日期:2006-02-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00770028
更新日期:1987-10-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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更新日期:2015-10-01 00:00:00
abstract::The original version of this article unfortunately contained a mistake. The name of "Zohreh Noaparast" is now corrected in the author group of this article. ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 已发布勘误
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更新日期:2019-08-01 00:00:00
abstract::Uncoupling proteins (UCPs) located in the inner mitochondrial membrane are involved in the regulation of energy balance. Thus far, 5 UCP isoforms have been identified, but controversies exist in the research focused on the function of the UCPs (except UCP1) in the pathogenesis of obesity. Because of the known cross-re...
journal_title:Journal of bioenergetics and biomembranes
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abstract::Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40 degrees C. When the cells are in the transformed state (grown a...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00769639
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abstract::Methylmalonic acidemia is one of the most prevalent inherited metabolic disorders involving neurological deficits. In vitro experiments, animal model studies and tissue analyses from human patients suggest extensive impairment of mitochondrial energy metabolism in this disease. This review summarizes changes in mitoch...
journal_title:Journal of bioenergetics and biomembranes
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abstract::Cytochrome c oxidase was purified from mitochondria of Euglena gracilis and separated into 15 different polypeptide subunits by polyacrylamide gel electrophoresis. All 15 subunits copurify through various purification procedures, and the subunit composition of the isolated enzyme is identical to that of the immunoprec...
journal_title:Journal of bioenergetics and biomembranes
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abstract::Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with Vibrio fischeri, V. harveyi, and Photobacterium ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1010615508916
更新日期:2001-08-01 00:00:00
abstract::Bioenergetics of tomato (Lycopersicon esculentum) development on the plant was followed from the early growing stage to senescence in wild type (climacteric) and nonripening mutant (nor, non-climacteric) fruits. Fruit development was expressed in terms of evolution of chlorophyll a content allowing the assessment of a...
journal_title:Journal of bioenergetics and biomembranes
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doi:10.1023/a:1022574327117
更新日期:2002-12-01 00:00:00
abstract::Phosphatase activity of a kidney (Na + K)-ATPase preparation was optimally active with Mg2+ plus K+. Mn2+ was less effective and Ca2+ could not substitute for Mg2+. However, adding Ca2+ with Mg2+ or substituting Mn2+ for Mg2+ activated it appreciably in the absence of added K+, and all three divalent cations decreased...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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