Abstract：
:Heat shock protein 90 (Hsp90) is an attractive therapeutic target. Geldanamycin (GA), the first identified Hsp90 inhibitor, exhibited potent antitumor activity, but possessed significant hepatotoxicity. To overcome the hepatotoxicity derived from the quinone structure of GA, a non-quinone GA derivative 17-demethoxy-reblastatin (17-DR) was obtained from a genetically modified strain of Streptomyces hygroscopicus. In the present study, we examined the anticancer effects of 17-DR on human hepatocellular carcinoma (HCC) cell lines HepG2 and SMMC7721, and its underlying mechanisms. The results indicated that 17-DR could concentration-dependently inhibit the proliferation, and decrease the colony formation in HCC cells. It also induced significant apoptosis in HCC cells, which was mediated by mitochondria via a caspase-dependent pathway. The mechanisms involved in 17-DR-induced apoptosis included the downregulation of myeloid cell leukemia-1 (Mcl-1), and upregulation of Bcl-2 antagonist killer 1 (Bak). And the upregulated Bak expression resulted from downregulation of Mcl-1 played an essential role in this process. Taken together, these results indicated that 17-DR possessed potent anticancer effects on HCC cells by inhibiting cell proliferation and inducing apoptosis.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Zhao S,Li H,Jiang C,Ma T,Wu C,Huo Q,Liu Hdoi
10.1007/s10863-015-9620-1subject
Has Abstractpub_date
2015-10-01 00:00:00pages
373-81issue
5eissn
0145-479Xissn
1573-6881pii
10.1007/s10863-015-9620-1journal_volume
47pub_type
杂志文章abstract：:This study was designed to investigate the energetics of isolated rat hepatocyte swelling due to sodium-cotransported amino acid accumulation in a medium containing either glucose or octanoate as basal substrate. We show that the size of the increase in cytosolic volume is directly correlated with the total amino acid...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1022487119390
更新日期：1997-12-01 00:00:00
abstract：:Bioenergetics of tomato (Lycopersicon esculentum) development on the plant was followed from the early growing stage to senescence in wild type (climacteric) and nonripening mutant (nor, non-climacteric) fruits. Fruit development was expressed in terms of evolution of chlorophyll a content allowing the assessment of a...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1022574327117
更新日期：2002-12-01 00:00:00
abstract：:Available data indicate that minocycline, an antibiotic of the tetracycline family, has cytoprotective properties due to a direct interaction with mitochondria. Yet, the data in the case of isolated mitochondria suggest discrepant or even detrimental effect(s) of the interaction. We have studied the cytoprotective act...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-012-9441-4
更新日期：2012-06-01 00:00:00
abstract：:The small alkylating molecule, 3-bromopyruvate (3BP), is a potent and specific anticancer agent. 3BP is different in its action from most currently available chemo-drugs. Thus, 3BP targets cancer cells' energy metabolism, both its high glycolysis ("Warburg Effect") and mitochondrial oxidative phosphorylation. This inh...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-012-9417-4
更新日期：2012-02-01 00:00:00
abstract：:The past 5 years has witnessed an advance in our understanding of alpha1G (Cav3.1), alpha1H (Cav3.2), and alpha11 (Car3.3), the pore-forming subunits of T-type or low-voltage-activated calcium channels (LVAs). LVAs differ in their localization and molecular, biophysical, and biochemical properties, but all conduct a t...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/b:jobb.0000008024.77488.48
更新日期：2003-12-01 00:00:00
abstract：:Cytoplasmic organelles with an acidic luminal pH include vacuoles, coated vesicles, lysosomes, the Golgi apparatus, and synaptic vesicles. Acidic compartments are also known outside specialized cells such as osteoclasts. The unique acidic pH is formed by V-ATPase (Vacuolar type ATPase), other ion transporters, and the...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1025780932403
更新日期：2003-08-01 00:00:00
abstract：:A great deal of progress has been made in understanding both the structure and the mechanism of F1-ATPase. The primary structure is now fully known for at least five species. Sequence comparison between chloroplast, photobacteria, aerobic bacteria, and mitochondrial representatives allow us to infer more general funct...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00762202
更新日期：1988-08-01 00:00:00
abstract：:The role of nucleoside diphosphate (NDP) kinase with special reference to mammalian signal transduction systems was described. The interaction between NDP kinases and G proteins was reevaluated in view of their protein structural information and its significance was extended further on the basis of recent findings obt...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1023489722460
更新日期：2003-02-01 00:00:00
abstract：:Complex I (NDH-1) translocates protons across the membrane using electron transfer energy. Two different coupling mechanisms are currently being discussed for complex I: direct (redox-driven) and indirect (conformation-driven). Semiquinone (SQ) intermediates are suggested to be key for the coupling mechanism. Recently...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-014-9557-9
更新日期：2014-08-01 00:00:00
abstract：:The role of the adenine nucleotide translocase on Ca2+ homeostasis in mitochondria from brown adipose tissue was examined. It was found that in mitochondria incubated with 50 microM Ca2+, ADP was not needed to retain the cation, but it was required for strengthening the inhibitory effect of cyclosporin on membrane per...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Changes in protein and fatty acid compositions of flounder sarcoplasmic reticulum during NADH plus ascorbate-dependent lipid peroxidation in vitro were related to the ability of the sarcoplasmic reticulum to sequester Ca+2. Progressive accumulation of high-molecular-weight protein components occurred concomitantly wit...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00743052
更新日期：1986-08-01 00:00:00
abstract：:Liver mitochondria isolated from rats 24 h after a single subcutaneous injection of 8 mg thyroxine per kilogram body weight were compared with those isolated from control rats that received injections of isotonic saline at the same time. The mitochondria isolated from the thyroxine-treated rats show higher rates of en...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00748766
更新日期：1980-12-01 00:00:00
abstract：:The organization of the oxidative phosphorylation (OXPHOS) system within the inner mitochondrial membrane appears to be far more complicated than previously thought. In particular, the individual protein complexes of the OXPHOS system (complexes I to V) were found to specifically interact forming defined supramolecula...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-008-9167-5
更新日期：2008-10-01 00:00:00
abstract：:Epilepsy is considered one of the most common neurological disorders worldwide. The burst firing neurons associated with prolonged epileptic discharges could lead to a large number of changes with events of cascades at the cellular level. From its role as the cellular powerhouse, mitochondria also play a crucial role ...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-010-9321-8
更新日期：2010-12-01 00:00:00
abstract：:The protons excreted by plant cells may arise by two different mechanisms: (1) by the action of the plasma membrane H(+)-ATPase and (2) by plasma membrane redox reactions. The exact proportion from each source is not known, but the plasma membrane H(+)-ATPase is, by far, the major contributor to proton efflux. There i...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00771014
更新日期：1991-06-01 00:00:00
abstract：:Calcium may function directly in several aspects of photosynthesis. It appears to modulate activity of the phosphatase enzymes in the carbon reduction cycle and also to regulate chloroplast NAD+ kinase activity through a calmodulin-like protein. Some evidence supports a calcium function in the water-splitting complex,...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00744278
更新日期：1984-08-01 00:00:00
abstract：:The effect of triphenyltin on mitochondrial Ca2+ content was studied. It was found that this trialkyltin compound induces an increase in membrane permeability that leads to Ca2+ release, drop of the transmembrane potential, and efflux of matrix proteins. Interestingly, cyclosporin A was unable to inhibit triphenyltin-...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00762786
更新日期：1994-08-01 00:00:00
abstract：:In this short review, we summarize our work on the role of members of the FXYD protein family as tissue-specific modulators of Na, K-ATPase. FXYD1 or phospholemman, mainly expressed in heart and skeletal muscle increases the apparent affinity for intracellular Na(+) of Na, K-ATPase and may thus be important for approp...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-005-9476-x
更新日期：2005-12-01 00:00:00
abstract：:This review is primarily focused on the contribution of our laboratory to study of the mitochondrial uncoupling UCPs. The initial stage was the description of a 32-kDa membranous protein specifically induced in brown adipose tissue mitochondria of cold-adapted rats. This protein was then shown by others to be responsi...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1005488105076
更新日期：1999-10-01 00:00:00
abstract：:The formation of paracrystalline inclusions in mitochondria following postmortem aging in situ is further investigated. Inclusions are found to localize either in the mitochondrial intracristal space or between the inner and outer membrane. The greater majority of the inclusions are of the intracristal type. pH is not...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00743275
更新日期：1977-04-01 00:00:00
abstract：:We have studied a mutant strain of Drosophila in which 80% of the mitochondrial DNA molecules have lost over 30% of their coding region through deletion. This deletion affects genes encoding five subunits of complex I of the respiratory chain (NADH:ubiquinone oxidoreductase). The enzymatic activity of complex I in the...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/b:jobb.0000023624.06665.40
更新日期：2004-04-01 00:00:00
abstract：:Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very noisy, substantial improvement of image quality can be obtained by averaging individual projections. Crystallographic and...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00763217
更新日期：1994-02-01 00:00:00
abstract：:Gramicidin A forms ion-conducting channels which can traverse the hydrocarbon core of lipid bilayer membranes. The structures formed by gramicidin A are among the best characterized of all membrane-bound polypeptides or proteins. In this review a brief summary is given of the occurrence, conformation, and synthesis of...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00762301
更新日期：1987-12-01 00:00:00
abstract：:Mitochondria are dynamic structures for which fusion and fission are well characterized for rapidly dividing cells in culture. Based on these data, it has recently been proposed that high respiratory activity is the result of fusion and formation of mitochondrial reticulum, while fission results in fragmented mitochon...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-012-9494-4
更新日期：2013-08-01 00:00:00
abstract：:Research on VDAC has accelerated as evidence grows of its importance in mitochondrial function and in apoptosis. New investigators entering the field are often confounded by the VDAC literature and its many apparent conflicts and contradictions. This review is an effort to shed light on the situation and identify reli...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-005-6566-8
更新日期：2005-06-01 00:00:00
abstract：:The total amount of cellular mitochondrial DNA (mtDNA) varies widely and seems to be related to the nature and metabolic state of tissues and cells in culture. It is not known, however, whether this variation has any significance in vivo, and to which extent it regulates energy production. To better understand the imp...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-008-9130-5
更新日期：2008-04-01 00:00:00
abstract：:Plasma membrane vesicles of HeLa cells are characterized by a drug-responsive oxidation of NADH. The NADH oxidation takes place in an argon or nitrogen atmosphere and in samples purged of oxygen. Direct assay of protein thiols by reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB; Ellman's reagent), suggests tha...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1020594214379
更新日期：1998-10-01 00:00:00
abstract：:Recent advances in structural biology underlying mechanisms of channel gating have strengthened our knowledge about how K(+) channels can be inter-convertible between conductive and non-conductive states. We have reviewed and combined mutagenesis with biochemical, biophysical and structural information in order to und...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-012-9407-6
更新日期：2012-02-01 00:00:00
abstract：:The hexosamine biosynthetic pathway (HBP) integrates glucose, amino acids, fatty acids and nucleotides metabolisms for uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) synthesis. UDP-GlcNAc is the nucleotide sugar donor for O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) processes. O-GlcNAc transferase (OGT...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-018-9747-y
更新日期：2018-06-01 00:00:00
abstract：:Renal artery clamping itself induces renal ischemia which subsequently causes renal cell injury and can lead to renal failure. The duration of warm ischemia that would be safe for postoperative kidney function during partial nephrectomy remains under investigations. Mitochondria play an important role in pathophysiolo...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-016-9643-2
更新日期：2016-02-01 00:00:00
