17-Demethoxy-reblastatin, an Hsp90 inhibitor, induces mitochondria-mediated apoptosis through downregulation of Mcl-1 in human hepatocellular carcinoma cells.


:Heat shock protein 90 (Hsp90) is an attractive therapeutic target. Geldanamycin (GA), the first identified Hsp90 inhibitor, exhibited potent antitumor activity, but possessed significant hepatotoxicity. To overcome the hepatotoxicity derived from the quinone structure of GA, a non-quinone GA derivative 17-demethoxy-reblastatin (17-DR) was obtained from a genetically modified strain of Streptomyces hygroscopicus. In the present study, we examined the anticancer effects of 17-DR on human hepatocellular carcinoma (HCC) cell lines HepG2 and SMMC7721, and its underlying mechanisms. The results indicated that 17-DR could concentration-dependently inhibit the proliferation, and decrease the colony formation in HCC cells. It also induced significant apoptosis in HCC cells, which was mediated by mitochondria via a caspase-dependent pathway. The mechanisms involved in 17-DR-induced apoptosis included the downregulation of myeloid cell leukemia-1 (Mcl-1), and upregulation of Bcl-2 antagonist killer 1 (Bak). And the upregulated Bak expression resulted from downregulation of Mcl-1 played an essential role in this process. Taken together, these results indicated that 17-DR possessed potent anticancer effects on HCC cells by inhibiting cell proliferation and inducing apoptosis.


J Bioenerg Biomembr


Zhao S,Li H,Jiang C,Ma T,Wu C,Huo Q,Liu H




Has Abstract


2015-10-01 00:00:00














  • Ionic channels in the plasma membrane of Schizosaccharomyces pombe: evidence from patch-clamp measurements.

    abstract::Patch-clamp studies of the yeast Schizosaccharomyces pombe reveal that the plasma membrane contains a voltage-gated channel mildly selective for potassium over sodium, lithium, and chloride. The channel exhibits several conductances with a maximum of 153 pS. The channel gates in the region of physiologically relevant ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Vacata V,Höfer M,Larsson HP,Lecar H

    更新日期:1993-02-01 00:00:00

  • Porin isoform 2 has a different localization in Drosophila melanogaster ovaries than porin 1.

    abstract::Eukaryotic porins or VDACs are a class of transmembrane proteins mainly localized in the outer mitochondrial membrane, whose function is to allow the diffusion of metabolites between the cytosol and the mitochondrion. In Drosophila melanogaster, as in other organisms, a small family of genes encoding porins has been d...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Specchia V,Guarino F,Messina A,Bozzetti MP,De Pinto V

    更新日期:2008-06-01 00:00:00

  • Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei F(O)F (1) ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia.

    abstract::In the mitochondrial F(O)F(1) ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Martinez-Cruz O,Garcia-Carreño F,Robles-Romo A,Varela-Romero A,Muhlia-Almazan A

    更新日期:2011-04-01 00:00:00

  • An integrated molecular modeling approach for the tryptase monomer-curcuminoid recognition analysis: conformational and bioenergetic features.

    abstract::Human mast cell tryptase has been shown as an activating enzyme in matrix degradation process. The previous study suggest that tryptase either alone or in joining with activation of metalloproteinases, can associate in extra cellular matrix damage and the possible destruction of the basement membrane resulting in phot...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Wongrattanakamon P,Ampasavate C,Sirithunyalug B,Jiranusornkul S

    更新日期:2018-12-01 00:00:00

  • Respiratory and TCA cycle activities affect S. cerevisiae lifespan, response to caloric restriction and mtDNA stability.

    abstract::We studied the importance of respiratory fitness in S. cerevisiae lifespan, response to caloric restriction (CR) and mtDNA stability. Mutants harboring mtDNA instability and electron transport defects do not respond to CR, while tricarboxylic acid cycle mutants presented extended lifespans due to CR. Interestingly, mt...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Tahara EB,Cezário K,Souza-Pinto NC,Barros MH,Kowaltowski AJ

    更新日期:2011-10-01 00:00:00

  • Cytochrome c oxidase of Euglena gracilis: purification, characterization, and identification of mitochondrially synthesized subunits.

    abstract::Cytochrome c oxidase was purified from mitochondria of Euglena gracilis and separated into 15 different polypeptide subunits by polyacrylamide gel electrophoresis. All 15 subunits copurify through various purification procedures, and the subunit composition of the isolated enzyme is identical to that of the immunoprec...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Brönstrup U,Hachtel W

    更新日期:1989-06-01 00:00:00

  • Nm23-H1/NDP kinase folding intermediates and cancer: a hypothesis.

    abstract::The Nm23-H1/nucleoside diphosphate (NDP) kinase A is a metastasis suppressor, besides its enzymatic activity. The mutant S120G has been found in high-grade neuroblastomas. The mutant protein, once denatured in urea, is unable to refold in vitro. A size-exclusion chromatography analysis of the folding/association pathw...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Lascu I

    更新日期:2006-08-01 00:00:00

  • Membrane potential and delta pH dependency of reverse electron transport-associated hydrogen peroxide production in brain and heart mitochondria.

    abstract::Succinate-driven reverse electron transport (RET) is one of the main sources of mitochondrial reactive oxygen species (mtROS) in ischemia-reperfusion injury. RET is dependent on mitochondrial membrane potential (Δψm) and transmembrane pH difference (ΔpH), components of the proton motive force (pmf); a decrease in Δψm ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Komlódi T,Geibl FF,Sassani M,Ambrus A,Tretter L

    更新日期:2018-10-01 00:00:00

  • Implications of cytochrome b6/f location for thylakoidal electron transport.

    abstract::The cytochrome b6/f complex of higher plant chloroplasts is uniformly distributed throughout both appressed and nonappressed thylakoids, in contrast to photosystem II and photosystem I, the other major membrane protein complexes involved in electron transport. We discuss how this distribution is likely to affect inter...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Allred DR,Staehelin LA

    更新日期:1986-10-01 00:00:00

  • Mutations in the Atp1p and Atp3p subunits of yeast ATP synthase differentially affect respiration and fermentation in Saccharomyces cerevisiae.

    abstract::ATP1-111, a suppressor of the slow-growth phenotype of yme1Delta lacking mitochondrial DNA is due to the substitution of phenylalanine for valine at position 111 of the alpha-subunit of mitochondrial ATP synthase (Atp1p in yeast). The suppressing activity of ATP1-111 requires intact beta (Atp2p) and gamma (Atp3p) subu...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Francis BR,White KH,Thorsness PE

    更新日期:2007-04-01 00:00:00

  • Low-voltage-activated ("T-Type") calcium channels in review.

    abstract::The past 5 years has witnessed an advance in our understanding of alpha1G (Cav3.1), alpha1H (Cav3.2), and alpha11 (Car3.3), the pore-forming subunits of T-type or low-voltage-activated calcium channels (LVAs). LVAs differ in their localization and molecular, biophysical, and biochemical properties, but all conduct a t...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Yunker AM,McEnery MW

    更新日期:2003-12-01 00:00:00

  • The composition of the Bacillus subtilis aerobic respiratory chain supercomplexes.

    abstract::Bacillus subtilis has a bifurcated respiratory chain composed of a cytochrome branch and a quinol oxidase branch. The respiratory complexes of this bacterium have been elucidated mostly by the analysis of the genome and by the isolation of individual complexes. The supramolecular organization of this respiratory chain...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: García Montes de Oca LY,Chagolla-López A,González de la Vara L,Cabellos-Avelar T,Gómez-Lojero C,Gutiérrez Cirlos EB

    更新日期:2012-08-01 00:00:00

  • Kinetics of light emission and oxygen consumption by bioluminescent bacteria.

    abstract::Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with Vibrio fischeri, V. harveyi, and Photobacterium ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Bourgois JJ,Sluse FE,Baguet F,Mallefet J

    更新日期:2001-08-01 00:00:00

  • Pores from mitochondrial outer membranes of yeast and a porin-deficient yeast mutant: a comparison.

    abstract::Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. The addition of the porin resulted in a strong increase of the membrane conductance, which was caused ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Benz R,Schmid A,Dihanich M

    更新日期:1989-08-01 00:00:00

  • Effects of pyridoxal phosphate treatment on the (Na + K)-ATPase.

    abstract::Reaction of a dog kidney (Na + K)-ATPase with pyridoxal phosphate, followed by borohydride reduction, reduced the catalytic activity when measured subsequently. The time course of inactivation did not follow a first-order process, and certain characteristics of the residual enzymatic activity were modified. Moreover, ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Robinson JD

    更新日期:1984-06-01 00:00:00

  • Chemiosmotic concept of the membrane bioenergetics: what is already clear and what is still waiting for elucidation?

    abstract::The present state of the chemiosmotic concept is reviewed. Special attention is paid to (i) further progress in studies on the Na(+)-coupled energetics and (ii) paradoxical bioenergetic effects when protonic or sodium potentials are utilized outside the coupling membrane (TonB-mediated uphill transports across the out...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Skulachev VP

    更新日期:1994-12-01 00:00:00

  • Human cytochrome c oxidase: structure, function, and deficiency.

    abstract::As the terminal component of the mitochondrial respiratory chain, cytochrome c oxidase plays a vital role in cellular energy transformation. Human cytochrome c oxidase is composed of 13 subunits. The three major subunits form the catalytic core and are encoded by mitochondrial DNA (mtDNA). The remaining subunits are n...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Taanman JW

    更新日期:1997-04-01 00:00:00

  • TIPE2 suppresses progression and tumorigenesis of the oral tongue squamous cell carcinoma by regulating FoxP3+ regulatory T cells.

    abstract::To discover the effect of tumor necrosis factor-alpha-induced protein 8-like 2 (TIPE2) on the oral tongue squamous cell carcinoma (OTSCC) via affecting FoxP3+ regulatory T (Treg) cells. Immunohistochemistry was conducted to examine TIPE2 and FoxP3 expressions in OTSCC tumor tissues and corresponding oral mucosa. Tca81...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Zhao LL

    更新日期:2020-08-01 00:00:00

  • Morphology of proteoliposomes containing fluorescein-phosphatidylethanolamine reconstituted with native and subunit III-depleted cytochrome c oxidase.

    abstract::Beef heart cytochrome c oxidase was reconstituted in asolectin liposomes containing the pH indicator fluorescein-phosphatidylethanolamine (FPE) by the cholate-dialysis procedure. The influence of FPE on the asolectin liposome size and of the removal of subunit III from the complex on its incorporation into liposomes w...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Müller M,Azzi A

    更新日期:1985-12-01 00:00:00

  • Size and selectivity of gap junction channels formed from different connexins.

    abstract::Gap junction channels have long been viewed as static structures containing a large-diameter, aqueous pore. This pore has a high permeability to hydrophilic molecules of approximately 900 daltons in molecular weight and a weak ionic selectivity. The evidence leading to these conclusions is reviewed in the context of m...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Veenstra RD

    更新日期:1996-08-01 00:00:00

  • Cancer cell bioenergetics and pH regulation influence breast cancer cell resistance to paclitaxel and doxorubicin.

    abstract::The multidrug resistance (MDR) phenotype, frequently observed during cancer treatment, is often associated with drug efflux pump activity. However, many other factors are also known to be involved. Cancer cells often rely on aerobic glycolysis for energy production; this is known as the "Warburg effect" and is used as...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Tavares-Valente D,Baltazar F,Moreira R,Queirós O

    更新日期:2013-10-01 00:00:00

  • H+-dependent efflux of Ca2+ from heart mitochondria.

    abstract::A rapid loss of accumulated Ca2+ is produced by addition of H+ to isolated heart mitochondria. The H+-dependent Ca+ efflux requires that either (a) the NAD(P)H pool of the mitochondrion be oxidized, or (b) the endogenous adenine nucleotides be depleted. The loss of Ca2+ is accompanied by swelling and loss of endogenou...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Jurkowitz MS,Brierley GP

    更新日期:1982-12-01 00:00:00

  • Transformation with SV40 virus prevents retinoic acid inhibition of plasma membrane NADH diferric transferrin reductase in rat liver cells.

    abstract::Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40 degrees C. When the cells are in the transformed state (grown a...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Sun IL,Toole-Simms W,Crane FL,Morré DJ,Löw H,Chou JY

    更新日期:1988-06-01 00:00:00

  • Control of ubiquinol oxidation at center P (Qo) of the cytochrome bc1 complex.

    abstract::The unique bifurcated oxidation of ubiquinol at center P (Qo) of the cytochrome bc1 complex is the reaction within the Q-cycle reaction scheme that is most critical for the link between electron transfer and vectorial proton translocation. While there is a general consensus about the overall reaction at center P, the ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Brandt U

    更新日期:1999-06-01 00:00:00

  • Role of chloride ion in hydroxyl radical production in photosystem II under heat stress: electron paramagnetic resonance spin-trapping study.

    abstract::Hydroxyl radical (HO•) production in photosystem II (PSII) was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that the exposure of PSII membranes to heat stress (40 °C) results in HO• formation, as monitored by the formation of EMPO-OH adduct EPR signal. The presence ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Yadav DK,Pospíšil P

    更新日期:2012-06-01 00:00:00

  • The road to the crystal structure of the cytochrome bc1 complex from the anoxigenic, photosynthetic bacterium Rhodobacter sphaeroides.

    abstract::The advantages of using bacterial systems to study the mechanism and function of cytochrome bc (1) complexes do not extend readily to their structural investigations. High quality crystals of bacterial complexes have been difficult to obtain despite the enzymes' smaller sizes and simpler subunit compositions compared ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Xia D,Esser L,Elberry M,Zhou F,Yu L,Yu CA

    更新日期:2008-10-01 00:00:00

  • The reconstitution of oxidative phosphorylation in mitochondria isolated from a ubiquinone-deficient mutant of Saccharomyces cerevisiae.

    abstract::Mitochondria, isolated from the ubiquinone-deficient nuclear mutant of Saccharomyces cerevisiae E3-24, are practically unable to oxidize exogenous substrates. Respiratory activity, coupled to ATP synthesis, can, however, be reconstituted by the simple addition of ethanolic solutions of ubiquinones. A minimal length of...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: De Santis A,Bertoli E,Di Gioia A,Melandri BA,Baccarini Melandri A

    更新日期:1982-06-01 00:00:00

  • Effects of methylglyoxal and pyridoxamine in rat brain mitochondria bioenergetics and oxidative status.

    abstract::Advanced glycation end products (AGEs) and methylglyoxal (MG), an important intermediate in AGEs synthesis, are thought to contribute to protein aging and to the pathogenesis of age-and diabetes-associated complications. This study was intended to investigate brain mitochondria bioenergetics and oxidative status of ra...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Cardoso S,Carvalho C,Marinho R,Simões A,Sena CM,Matafome P,Santos MS,Seiça RM,Moreira PI

    更新日期:2014-10-01 00:00:00

  • Inclusions in aged mitochondria.

    abstract::The formation of paracrystalline inclusions in mitochondria following postmortem aging in situ is further investigated. Inclusions are found to localize either in the mitochondrial intracristal space or between the inner and outer membrane. The greater majority of the inclusions are of the intracristal type. pH is not...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Cheah KS,Cheah AM

    更新日期:1977-04-01 00:00:00

  • Stimulation of electron transport from photosystem II to photosystem I in spinach chloroplasts.

    abstract::Electron transport from Photosystem II to Photosystem I of spinach chloroplasts can be stimulated by bicarbonate and various carbonyl or carboxyl compounds. Monovalent or divalent cations, which have hitherto been implicated in the energy distribution between the two photosystems, i.e., "spillover" phenomena at low li...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Barr R,Melhem R,Lezotte AL,Crane FL

    更新日期:1980-08-01 00:00:00