Abstract：
RATIONALE AND OBJECTIVES:The authors performed this study to determine whether intrathoracic inoculation of non-small-cell lung carcinoma with fluoroscopic guidance would provide for more accurate implantation. MATERIALS AND METHODS:A tumor cell inoculum (2 x 10(6) cells per 0.15 mL) was injected percutaneously under fluoroscopic guidance at the posterior midaxillary line in 22 athymic nude mice. The mice underwent imaging with a mammographic unit at 3, 5, and 8 weeks after implantation. The mice were sacrificed at 8 weeks, and autopsy was performed to determine tumor yield. RESULTS:The use of a percutaneous technique under fluoroscopic guidance greatly facilitated the accurate implantation of xenografts. Tumor growth was seen at radiography in 18 of the 22 (82%) mice at 8 weeks. Necropsy revealed a 100% tumor yield. Histologic examination confirmed adenocarcinoma of the lung. The average number of tumors found in the lung parenchyma was 1.05 +/- 0.35; the average number of tumors found in the mediastinum was 0.59 +/- 0.67. The average tumor weight was 389 mg +/- 64.3. The average tumor size was 300 mm3 +/- 66.23. CONCLUSION:With fluoroscopic guidance, percutaneous implantation of tumor cells in athymic nude mice is simple and effective.
journal_name
Acad Radioljournal_title
Academic radiologyauthors
Hamide JP,Qian Z,Xu H,Diethelm L,Skrepnik N,Castaneda-Zuniga WR,Hunt JDdoi
10.1016/s1076-6332(05)80267-3subject
Has Abstractpub_date
1997-09-01 00:00:00pages
629-33issue
9eissn
1076-6332issn
1878-4046journal_volume
4pub_type
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