Abstract:
:Unfertilized eggs and fertilized embryos from Drosophila mothers mutant for the plutonium (plu) gene contain giant polyploid nuclei resulting from unregulated S-phase. The PLU protein, a 19-kDa ankyrin repeat protein, is present in oocytes and early embryos but is not detectable after the completion of the initial rapid S-M cycles of the embryo. The persistence of the protein during the early embryonic divisions is consistent with a direct role in linking S-phase and M-phase. When ectopically expressed in the eye disc, PLU did not perturb the cell cycle, suggesting that PLU regulates S-phase only in early embryonic development. The pan gu (png) and giant nuclei (gnu) genes also affect the S-phase in the unfertilized egg and early embryo. We show that functional png is needed for the presence of PLU protein. By analyzing png mutations of differing severity, we find that the extent of the png mutant phenotype inversely reflects the level of PLU protein. Our data suggest that PLU protein is required at the time of egg activation and the completion of meiosis.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Elfring LK,Axton JM,Fenger DD,Page AW,Carminati JL,Orr-Weaver TLdoi
10.1091/mbc.8.4.583subject
Has Abstractpub_date
1997-04-01 00:00:00pages
583-93issue
4eissn
1059-1524issn
1939-4586journal_volume
8pub_type
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